SiRNA Mediated Gene Silencing in the Pancreatic Cancer Capan-1 Cell Line

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dc.contributor.authorYıldıran, Fatma Azize Budak
dc.date.accessioned2025-01-21T16:13:48Z
dc.date.available2025-01-21T16:13:48Z
dc.date.issued2022
dc.departmentKırıkkale Üniversitesi
dc.description.abstractİngilizce Today, cancer is the second leading among the causes of death. Especially pancreatic cancer which is an aggressive type progressing without giving too many symptoms. In addition, non-response to traditional treatments cause a low survival rate in this type of cancer. Therefore, many target-oriented studies are carried out to develop alternative diagnosis and treatment methods such as gene therapy method. Small non-coding RNA (siRNA) are used in studies as a therapeutic agent by its delivery to the target gene by various mechanisms. This study aimed to silence the c-Myc gene using gold nanoparticle (AuNP)-siRNA in the Capan-1 cell line. We performed Real-Time PCR analysis, WST-1 assay in the cytotoxicity analysis, Double Staining method and Agarose gel electrophoresis using gold nanoparticles (2X, 4X, 8X) and siRNA (25 nM) at concentrations determined in the Capan-1 cell line. The data obtained by Real Time PCR were evaluated as relative (Efficiency 2). 2?Ct values were calculated. The 2?Ct value was 0.352 for the control, and these values were found to be between 0.210-0.027 in AuNP / siRNA applications. Considering these values, it was observed that the gene expression decreased the most for 8XAuNP/siRNA. The averages of the OD values (438 nm) read in the WST-1 analysis were calculated. These values were calculated as 0.180 for the control group, 0.194 for 2XAuNP/siRNA, 0.293 for 4XAuNP/siRNA, and 0.277 for 8XAuNP/siRNA. The data obtained from the analysis suggested that the nanoparticle siRNA complex could be used effectively in silencing the target gene. Nevertheless, more knowledge is needed on this subject.
dc.identifier.doi10.46810/tdfd.109743
dc.identifier.endpage103
dc.identifier.issn2149-6366
dc.identifier.issue4
dc.identifier.startpage98
dc.identifier.trdizinid1147970
dc.identifier.urihttps://doi.org/10.46810/tdfd.109743
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1147970
dc.identifier.urihttps://hdl.handle.net/20.500.12587/22009
dc.identifier.volume11
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofTürk Doğa ve Fen Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241229
dc.subjectTıbbi İnformatik
dc.subjectTıbbi Araştırmalar Deneysel
dc.subjectMikrobiyoloji
dc.subjectOnkoloji
dc.subjectBiyokimya ve Moleküler Biyoloji
dc.titleSiRNA Mediated Gene Silencing in the Pancreatic Cancer Capan-1 Cell Line
dc.typeArticle

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