Morphometric, immunohistochemical and ultrastructural examination of age-related structural alterations in optic nerve

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dc.contributor.authorAnlı, Serpil Çilingiroğlu
dc.contributor.authorÇalgüner, Engin
dc.contributor.authorErdoğan, Deniz
dc.contributor.authorKadıoğlu, Dural
dc.contributor.authorElmas, Çiğdem
dc.contributor.authorGozil, Rabet
dc.contributor.authorBahçelioğlu, Meltem
dc.date.accessioned2025-01-21T16:12:14Z
dc.date.available2025-01-21T16:12:14Z
dc.date.issued2024
dc.departmentKırıkkale Üniversitesi
dc.description.abstractAims: As individuals age, there is a known decline in visual function attributed to a reduction in the optic nerve fibers and myelin sheath degeneration. Studies present conflicting findings on whether aging affects axonal integrity in the human optic nerve. This study aims to investigate degenerative changes in the aging rat optic nerve. Methods: The investigation involved 36 Wistar albino rats. The rats were divided into six groups: the newborn, prepubertal, pubertal, junior, adult, and elderly groups. This study investigated optic nerve axon counts, axon diameters, levels of glial fibrillary acidic protein immunoreactivity (GFAP-IR) and nerve growth factor immunoreactivity (NGF-IR), as well as findings from light microscopy (LM) and electron microscopy (EM) in these groups. Results: This study observed age-related alterations in rat optic nerves, including increased diameter, irregular axon count fluctuations (both increases and decreases), elevated astrocyte count, and a simultaneous decline in oligodendrocyte count. Additionally, it was observed that NGF-IR was predominantly at the membrane level in newborns and moderately in the cytoplasm, whereas in older ages, it was evident at both cellular and axonal levels furthermore, it was observed that GFAP-IR increased with age. However, in LM and EM examinations, axonal loss and rarefaction, accumulation of osmiophilic substances, splitting of the myelin sheath, vacuolization, axonal retraction were observed. Conclusion: In this study, it was found that one of the causes of age-related vision loss is the advanced degenerative changes in the optic nerve and it was concluded that the remaining small-diameter myelinated nerve fibers may partially compensate for the sense of vision. Our study reveals that age-related degenerative changes in the central nervous system resemble those in multiple sclerosis (MS), suggesting a potential contribution to MS pathogenesis.
dc.identifier.doi10.32322/jhsm.1486594
dc.identifier.endpage425
dc.identifier.issn2636-8579
dc.identifier.issue4
dc.identifier.startpage416
dc.identifier.trdizinid1268635
dc.identifier.urihttps://doi.org/10.32322/jhsm.1486594
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1268635
dc.identifier.urihttps://hdl.handle.net/20.500.12587/21661
dc.identifier.volume7
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofJournal of health sciences and medicine (Online)
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241229
dc.titleMorphometric, immunohistochemical and ultrastructural examination of age-related structural alterations in optic nerve
dc.typeArticle

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