Optimization Studies and Results of Recombinase Polymerase Amplification Technique for Gene Mutation Detection

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dc.contributor.authorÇağdaş, Beste
dc.contributor.authorKerstıng, Sebastian
dc.date.accessioned2025-01-21T16:13:42Z
dc.date.available2025-01-21T16:13:42Z
dc.date.issued2023
dc.departmentKırıkkale Üniversitesi
dc.description.abstractSingle nucleotide polymorphisms (SNPs) in human genes are very significant genetic changes and PCR (polymerase chain reaction) or NGS (next-generation sequencing) are extensively employed in SNP analysis. Thanks to the studies on the progress of new technologies, interest in the isothermal nucleic acid amplification approach has increased. As one of these methods, recombinase polymerase amplification (RPA) represents an attractive option for point-of-care nucleic acid quantification. The target SNPs selected within the scope of the study are mutations identified in the PIK3CA gene region (E542K, E545K), and DNA samples which were evaluated about PIK3CA mutations were isolated from the cancer cells MCF7, BT474, and also SKBr3. The optimization studies for the RPA reaction conditions were carried out for parameters such as assay time, temperature, primer, and also magnesium acetate concentration. According to the results of the reaction optimization studies, in which the RPA products can be obtained in the most efficient way, the assay time was determined as 20 min; the temperature as 40°C; the primer concentration as 10 µM and the MgOAc concentration as 140 mM.
dc.identifier.doi10.35414/akufemubid.1322267
dc.identifier.endpage1372
dc.identifier.issn2149-3367
dc.identifier.issue6
dc.identifier.startpage1363
dc.identifier.trdizinid1216191
dc.identifier.urihttps://doi.org/10.35414/akufemubid.1322267
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1216191
dc.identifier.urihttps://hdl.handle.net/20.500.12587/21985
dc.identifier.volume23
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofAfyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241229
dc.subjectTıbbi Araştırmalar Deneysel
dc.subjectBiyoteknoloji ve Uygulamalı Mikrobiyoloji
dc.subjectOnkoloji
dc.subjectBiyokimya ve Moleküler Biyoloji
dc.titleOptimization Studies and Results of Recombinase Polymerase Amplification Technique for Gene Mutation Detection
dc.typeArticle

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