Role of stellate cells in hepatic echinococcosis in cattle

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dc.contributor.authorAtmaca H.T.
dc.contributor.authorGazyagci A.N.
dc.contributor.authorTerzi O.S.
dc.contributor.authorSumer T.
dc.date.accessioned2020-06-25T15:18:05Z
dc.date.available2020-06-25T15:18:05Z
dc.date.issued2019
dc.departmentKırıkkale Üniversitesi
dc.description.abstractHydatid cysts formed by the metacestodes of Echinococcus granulosus. Cattle suffering from hydatid cyst shows fluid-filled structures, especially in liver. These parasite-induced cysts localized by forming fibrous capsules in the liver. Fibrogenesis is the host immune response in the liver against these parasites. Hepatic stellate cells (HSCs) are localized perisinusoidal space also known as vitamin A-storing cells, characterize the important fibrogenic cell type. In this study, livers from 15 animals with hydatid cyst and 8 healthy animals were used. Hematoxylin and Eosin, masson trichrome staining were performed on the prepared liver sections. Microscopically, cysts were bordered eosinophilic necrotic debris blended with degenerate neutrophils, macrophages, eosinophils, lymphocytes, plasma cells and multinucleated giant cells, which extend into the adjacent fibrous connective tissue. In Masson trichrome staining, the fibrous connective tissue was observed surrounding of hydatid cyst. Glial fibrillary acidic protein (GFAP), collagen I, GFAP/collagen I, positive cells were investigated using either indirect single- or double-labeling immunohistochemical staining. The results indicated that anti-GFAP-positive staining was seen in areas including fibrous tissue just under the foreign body giant cells surrounding the cyst wall. In double immunohistochemical staining, it was observed that HSCs labeled with anti-GFAP antibody in the fibrous connective tissue also labeled anti-collagen I antibody. This study shows that HSCs may responsible for synthesis the collagen I in the development of parasitic fibrosis in cystic echinococcosis in the liver of cattle. © 2019, Indian Society for Parasitology.en_US
dc.identifier.doi10.1007/s12639-019-01129-z
dc.identifier.endpage582en_US
dc.identifier.issn09717196
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85071882175
dc.identifier.scopusqualityQ3
dc.identifier.startpage576en_US
dc.identifier.urihttps://doi.org/10.1007/s12639-019-01129-z
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2618
dc.identifier.volume43en_US
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Parasitic Diseases
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCattleen_US
dc.subjectCollagen Ien_US
dc.subjectEchinococcosisen_US
dc.subjectGFAPen_US
dc.subjectHydatid cysten_US
dc.subjectImmunohistochemistryen_US
dc.titleRole of stellate cells in hepatic echinococcosis in cattleen_US
dc.typeArticle

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