Deneysel akut iskemik inme modelinde nörogranin ve glial fibriler asidik proteinin tanısal değeri
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Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Kırıkkale Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Giriş ve Amaç: Akut iskemik inme (Aİİ), dünya genelinde engellik ve mortalitenin ana nedenlerinden biridir. İnme hastasında tanının erken dönemde konması ve hızlı bir şekilde etkin tedavisinin başlatılmasıyla mortalite oranları azaltılabileceği gibi iyi klinik nörolojik sonuçlar sağlanabilir. Nörogranin (Ng) ve Glial Fibriller Asidik Protein (GFAP), nörolojik hasar durumunda serumda saptanabilen proteinlerdir. Bu çalışmanın amacı, Aİİ'de serum Ng ve GFAP düzeyi ölçümünün tanısal değerini ve histopatolojik verilerle olan ilişkisini araştırmaktır. Gereç ve Yöntem: Çalışmada ağırlıkları 250-350 g arasında değişen 24 adet Sprague-Dawley dişi rat kullanıldı. Ratlar her grupta 6'şar tane olacak şekilde 4 gruba ayrıldı. İskemi gruplarında sağ common carotid arter (CCA) bağlanarak iskemi modeli oluşturuldu. Grup CCA 1 : CCA bağlaması yapıldıktan 1 saat sonra örneklem alınan grup, Grup CCA 2 : CCA bağlaması yapıldıktan 2 saat sonra örneklem alınan grup, Grup CCA 6 : CCA bağlaması yapıldıktan 6 saat sonra örneklem alınan grup, Grup K: Kontrol grubu olarak tanımlandı. Tüm ratlar deney sonunda sakrifiye edilerek kan ve beyin dokusu örnekleri alındı. Serum örneklerinden Ng ve GFAP düzeyi ölçümü yapılırken, beyin dokusunda ise Hasarlı Nöron Yüzdesi (HNY)'nin oranı değerlendirlidi. Sonuçlar SPSS 21.0 programı ile analiz edildi. Bulgular: İskemi modeli oluşturulan tüm deney gruplarında serum Ng ve GFAP düzeyinin kontrol grubuna göre arttığı tespit edildi. Oluşturulan deney gruplarındaki serum Ng düzeyi, iskemi süreci ilerledikçe kademeli olarak tüm gruplarda yükseldi (Sırasıyla Grup CCA 1 vs Grup CCA 2 , p=0.002; Grup CCA 1 vs Grup CCA 6 , p<0.001; Grup CCA 2 vs Grup CCA 6 , p=0.041). Serum GFAP düzeyinin iskemi sürecinin uzamasıyla birlikte daha çok arttığı saptandı. İskemi grupları arasında GFAP düzeyi bakımından Grup CCA 1 ile Grup CCA 2 arasında fark saptanmazken (Grup CCA 1 vs Grup CCA 2 , p=0.132), diğer gruplar arasında anlamlı istatistiksel farklılık saptandı (Sırasıyla Grup CCA 1 vs Grup CCA 6 , p=0.002; Grup CCA 2 vs Grup CCA 6 , p=0.015). İskemi süreci uzadıkça HNY'nin kademeli olarak arttığı saptandı (Sırasıyla, Grup CCA 1 %42; Grup CCA 2 %51; Grup CCA 6 %57; Grup K %8.5)(Grup CCA 1 vs Grup CCA 2 , p=0.002; Grup CCA 1 vs Grup CCA 6 , p=0.002; Grup CCA 2 vs Grup CCA 6 , 11 p=0.004). İskemi oluşturulan deney gruplarındaki HNY, Kontrol grubundan anlamlı olarak daha yüksekti (Sırasıyla p=0.004; 0.004; 0.004). Serum Ng ve GFAP düzeyi ile HNY arasında pozitif yönlü korelasyon tespit edildi (Sırasıyla, r=0.700; p<0.001 ve r=0.700; p<0.001). Sonuç: İskemi süreci ilerledikçe ve HNY arttıkça serum Ng ve GFAP düzeyi artmaktadır. Bu artış Ng düzeyinde kademeli olarak gerçekleşmekteyken, GFAP'ta ise özellikle 6. saatte daha belirgindir. Hem Ng hem de GFAP, Aİİ hastalarının diliminde tanı ve nörolojik hasarı takip etmede kullanılabilecek biyobelirteçler olabilir. Anahtar Kelimeler: Hayvan deneyi, İskemik inme, Nörogranin, Glial Fibriler Asidik Protein, Nöron hasarı
Introduction and Aim: Acute ischemic stroke (AIS) is one of the leading global causes of disability and mortality. However, mortality rates can be reduced and good clinical outcomes achieved in stroke patients with early diagnosis and prompt initiation of effective treatment. Neurogranin (Ng) and glial fibrillary acidic protein (GFAP) are proteins capable of being detected in serum in case of neurological injury. The purpose of this study was to investigate the diagnostic value of serum Ng and GFAP levels in AIS and their relationship with histopathological data. Materials and Methods: Twenty-four female Sprague-Dawley rats weighing 250- 350 g were randomly divided into four groups containing six animals each. An ischemia model was established in the experimental groups by ligating the right common carotid artery (CCA). In Group CCA 1 samples were collected 1 h following CCA ligation, in Group CCA 2 samples were collected 2 h following CCA ligation, in Group CCA 6 samples were collected 6 h following CCA ligation, while Group K represented the control group. All rats were sacrificed at the end of the experiment, and blood and brain tissue samples were collected. Ng and GFAP levels were measured from serum samples, while of atrophic neuron percentage (ANP) values were evaluated in brain tissue. The results were analyzed on SPSS 21.0 software. Results: It was determined that serum Ng and GFAP levels increased in all experimental groups for which the ischemia model was performed compared to the control group. As the ischemia process progressed, serum Ng levels in the formed experimental groups gradually increased in all groups (Group CCA 1 vs Group CCA 2 , p=0.002; Group CCA 1 vs Group CCA 6 , p<0.001; Group CCA 2 vs Group CCA 6 , p=0.041, respectively). It was determined that the serum GFAP level increased more with the prolongation of the ischemia process. While there was no difference between Group CCA 1 and Group CCA 2 in terms of GFAP level between ischemia groups (Group CCA 1 vs Group CCA 2 , p=0.132), a statistically significant difference was found between the other groups (Group CCA 1 vs Group CCA 6 , p=0.002; Group 13 CCA 2 vs Group CCA 6 , p=0.015). It was determined that ANP gradually increased as the ischemia process prolonged (Group CCA 1 %42; Group CCA 2 %51; Group CCA 6 %57; Group K %8.5, respectively)(Group CCA 1 vs Group CCA 2 , p=0.002; Group CCA 1 vs Group CCA 6 , p=0.002; Group CCA 2 vs Group CCA 6 , p=0.004). ANP in the ischemia-induced experimental groups was significantly higher than in the Control group (p=0.004; 0.004; 0.004, respectively). A positive correlation was detected between serum Ng and GFAP levels and ANP (r=0.700; p<0.001 ve r=0.700; p<0.001, respectively). Conclusion: As the ischemia process progresses and ANP increases, serum Ng and GFAP levels increase. While this increase occurs gradually in Ng level, it is more evident in GFAP, especially at the 6th hour. Both Ng and GFAP may be biomarkers that can be used to diagnose and monitor neurological damage in patients with AIS. Keywords: Animal experiment, Ischemic stroke, Neurogranin, Glial Fibrillary Acidic Protein, Neuron injury
Introduction and Aim: Acute ischemic stroke (AIS) is one of the leading global causes of disability and mortality. However, mortality rates can be reduced and good clinical outcomes achieved in stroke patients with early diagnosis and prompt initiation of effective treatment. Neurogranin (Ng) and glial fibrillary acidic protein (GFAP) are proteins capable of being detected in serum in case of neurological injury. The purpose of this study was to investigate the diagnostic value of serum Ng and GFAP levels in AIS and their relationship with histopathological data. Materials and Methods: Twenty-four female Sprague-Dawley rats weighing 250- 350 g were randomly divided into four groups containing six animals each. An ischemia model was established in the experimental groups by ligating the right common carotid artery (CCA). In Group CCA 1 samples were collected 1 h following CCA ligation, in Group CCA 2 samples were collected 2 h following CCA ligation, in Group CCA 6 samples were collected 6 h following CCA ligation, while Group K represented the control group. All rats were sacrificed at the end of the experiment, and blood and brain tissue samples were collected. Ng and GFAP levels were measured from serum samples, while of atrophic neuron percentage (ANP) values were evaluated in brain tissue. The results were analyzed on SPSS 21.0 software. Results: It was determined that serum Ng and GFAP levels increased in all experimental groups for which the ischemia model was performed compared to the control group. As the ischemia process progressed, serum Ng levels in the formed experimental groups gradually increased in all groups (Group CCA 1 vs Group CCA 2 , p=0.002; Group CCA 1 vs Group CCA 6 , p<0.001; Group CCA 2 vs Group CCA 6 , p=0.041, respectively). It was determined that the serum GFAP level increased more with the prolongation of the ischemia process. While there was no difference between Group CCA 1 and Group CCA 2 in terms of GFAP level between ischemia groups (Group CCA 1 vs Group CCA 2 , p=0.132), a statistically significant difference was found between the other groups (Group CCA 1 vs Group CCA 6 , p=0.002; Group 13 CCA 2 vs Group CCA 6 , p=0.015). It was determined that ANP gradually increased as the ischemia process prolonged (Group CCA 1 %42; Group CCA 2 %51; Group CCA 6 %57; Group K %8.5, respectively)(Group CCA 1 vs Group CCA 2 , p=0.002; Group CCA 1 vs Group CCA 6 , p=0.002; Group CCA 2 vs Group CCA 6 , p=0.004). ANP in the ischemia-induced experimental groups was significantly higher than in the Control group (p=0.004; 0.004; 0.004, respectively). A positive correlation was detected between serum Ng and GFAP levels and ANP (r=0.700; p<0.001 ve r=0.700; p<0.001, respectively). Conclusion: As the ischemia process progresses and ANP increases, serum Ng and GFAP levels increase. While this increase occurs gradually in Ng level, it is more evident in GFAP, especially at the 6th hour. Both Ng and GFAP may be biomarkers that can be used to diagnose and monitor neurological damage in patients with AIS. Keywords: Animal experiment, Ischemic stroke, Neurogranin, Glial Fibrillary Acidic Protein, Neuron injury
Açıklama
Tıp Fakültesi, Acil Tıp Ana Bilim Dalı
Anahtar Kelimeler
Acil Tıp, Emergency Medicine
