Carotenoid Composition and Investigation of the Antioxidant Activity of Phormidium sp.

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dc.contributor.authorAluç, Yaşar
dc.date.accessioned2025-01-21T16:13:15Z
dc.date.available2025-01-21T16:13:15Z
dc.date.issued2022
dc.departmentKırıkkale Üniversitesi
dc.description.abstractMicroalgae metabolites are used for health, feed additives, cosmetic industries, food and biodiesel production. Phormidium species have an important position in medical studies because they contain essential components. In this study, carotenoid profile and content were analyzed using the HPLC method. Antioxidant activities for Phormidium sp. were determined using DPPH and FRAP assays. BHT and ASC were used as control samples in antioxidant assays. The method used to resolve a number of carotenoids from saponified Phormidium sp. proved acceptable separation, as evidenced by retention factor (k) values of 0.54 to 3.83 and separation factor (?) values greater than 1. Main carotenoids were dominated by the two main derivatives, all-trans form of lutein 41.35% (1.25 mg/g) and 9-or 9’-cis-?-carotene 36.43% (1.10 mg/g). Auroxanthin and cis neoxanthin were identified as epoxy-containing compounds. It is also understood that considering the DPPH assay, the extract of Phormidium sp. (IC50:127.6 mg/L) exhibited clearly low radical scavenging activity compared to the standards ASC (IC50: 0.02 mg/L) and BHT (IC50: 0.19 mg/L). In the FRAP antioxidant experiment, the mean ASC and BHT equivalent amounts were determined as 828.6 and 124.6 mg/L, respectively. Quantitatively, Phormidium sp. was predominated by cis-Lutein as a major constituent, being 41.35% (3.02 mg/g) in total carotenoids (Tc). The antioxidant capacity of Phormidium sp. that considering the DPPH and FRAP were compared to control standards were showed considerably low effects.
dc.identifier.doi10.33714/masteb.1106896
dc.identifier.endpage220
dc.identifier.issn2147-9666
dc.identifier.issue2
dc.identifier.startpage212
dc.identifier.trdizinid527112
dc.identifier.urihttps://doi.org/10.33714/masteb.1106896
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/527112
dc.identifier.urihttps://hdl.handle.net/20.500.12587/21882
dc.identifier.volume11
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofMarine Science and Technology Bulletin
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241229
dc.subjectBiyoloji
dc.subjectKimya
dc.subjectTıbbi
dc.subjectDeniz ve Tatlı Su Biyolojisi
dc.titleCarotenoid Composition and Investigation of the Antioxidant Activity of Phormidium sp.
dc.typeArticle

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