A research on Babesia and Theileria species in sheep and goats of Kırıkkale province through molecular methods

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dc.contributor.authorGökpınar, Sami
dc.contributor.authorGazyağcı, Aycan Nuriye
dc.contributor.authorAydenizöz, Meral
dc.contributor.authorKaya, Ufuk
dc.date.accessioned2025-01-21T16:16:03Z
dc.date.available2025-01-21T16:16:03Z
dc.date.issued2021
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe aim of the present study was to determine presence and prevalence of Babesia and Theileria species in the sheep and goats\rof stock farming in the province of Kırıkkale, which is in Central Anatolia Region of Turkey through microscopic examination and RLB\rmethod. Venous blood samples of 3 mL were collected from vena jugularis of 300 sheep and 100 goats into tubes containing EDTA.\rBlood smears were prepared from samples; and stained by Giemsa and examined for Theileria and Babesia under a light microscope.\rGenomic DNAs were extracted from blood samples with EDTA through suitable methods; a region with a length of 360 to 430 bp on\rvariable region V4 of the 18 ssu rRNA gene of Theileria and Babesia species were proliferated; PCR products obtained were hybridized\ron a membrane where species-specific probes. Piroplasms were detected in 54 (18%) sheep and in none of the goats during microscopic\rexamination of blood smears. With the RLB technique; T. ovis 70%, Theileria sp. 2.3%, B. ovis 5.3%, T. ovis + B. ovis 2.7% were detected\rin sheep. In goats; T. ovis was detected at a rate of 1%. Consequently, this study is the first for detection of Theileria and Babesia species\rin sheep and goats in Kırıkkale. Presence of T. ovis and B. ovis in the sheep, and T. ovis in goats was detected in Kırıkkale.
dc.identifier.doi10.3906/vet-2103-17
dc.identifier.endpage919
dc.identifier.issn1300-0128
dc.identifier.issn1303-6181
dc.identifier.issue5
dc.identifier.startpage912
dc.identifier.trdizinid528190
dc.identifier.urihttps://doi.org/10.3906/vet-2103-17
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/528190
dc.identifier.urihttps://hdl.handle.net/20.500.12587/22324
dc.identifier.volume45
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofTurkish Journal of Veterinary and Animal Sciences
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241229
dc.subjectBiyoloji
dc.subjectVeterinerlik
dc.titleA research on Babesia and Theileria species in sheep and goats of Kırıkkale province through molecular methods
dc.typeArticle

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