INVESTIGATION OF CYTOTOXIC AND ANTI-PROLIFERATIVE EFFECTS OF CRUDE SPIDER VENOM OF Agelena orientalis ON THE BREAST CANCER CELL LINE

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dc.contributor.authorKayhan, Nazife Yigit
dc.contributor.authorOcal, Ilkay Corak
dc.contributor.authorAkdeniz, Kader
dc.date.accessioned2025-01-21T16:41:52Z
dc.date.available2025-01-21T16:41:52Z
dc.date.issued2022
dc.departmentKırıkkale Üniversitesi
dc.description.abstractAmong the venomous animals, spiders have the highest species diversity. In literature, it was reported that spider venoms contain many chemicals including proteins, peptides, polyamine neurotoxins, enzymes, free nucleic acids, monoamines, and inorganic salts. Since spider venoms are rich in molecular content, they can have pharmacologically important molecules. Even though the spiders have important species diversity, only few studies examined the venomous spiders. In these studies, it was shown that spider venoms have cytotoxic, anti-tumoral, antibacterial, antiviral, and insecticidal effects. In the present study, the cytotoxic and anti-proliferative effects of the crude venom of Agelena orientalis C.L. Koch spider on the 1837 breast cancer cell line (MCF-7) and the mouse fibroblast cell line (L929) were examined. In cells treated with venom in cell culture medium, cytotoxic activity was analyzed using 3, [4,5-dimethylthiazol-2-y1]-2,5-diphenylte-trazolium bromide (MTT) test and apoptotic and necrotic cell death was analyzed by using Hoechst 33342 and PI double staining method. Moreover, the cell proliferation was examined by making use of real-time cell analyses system. As a result of the analyses, it was determined that crude spider venom had a cytotoxic effect on MCF-7 and fibroblast cells, and it induced apoptosis in these cells. It was also observed that the crude venom of this spider partially decelerated the proliferation of MCF-7 cells. The present study is the first study reporting the cytotoxic and anti-proliferative effects of A. orientalis spider's crude venom.
dc.identifier.endpage7759
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue8
dc.identifier.startpage7751
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24972
dc.identifier.volume31
dc.identifier.wosWOS:000845078600033
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherParlar Scientific Publications (P S P)
dc.relation.ispartofFresenius Environmental Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectAgelena orientalis; cancer; cell proliferation; cytotoxicity; spider venom
dc.titleINVESTIGATION OF CYTOTOXIC AND ANTI-PROLIFERATIVE EFFECTS OF CRUDE SPIDER VENOM OF Agelena orientalis ON THE BREAST CANCER CELL LINE
dc.typeArticle

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