Kronik aktif hepatit b hastalarında histopatolojik fibrozis evreleri ile glutatyon s transferaz izozimlerinin expresyonlarının karşılaştırılması
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Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Kırıkkale Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
ÖZET KRONİK AKTİF HEPATİT B HASTALARINDA HİSTOPATOLOJİK FİBROZİS EVRELERİ İLE GLUTATYON S TRANSFERAZ İZOZİMLERİNİN EXPRESYONLARININ KARŞILAŞTIRILMASI EROĞLU, Ergün Kırıkkale Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Anabilim Dalı, Yüksek Lisans Tezi Danışman: Prof. Dr. Serpil OĞUZTÜZÜN Ocak 2020, 43 sayfa Bu çalışmada, kronik aktif hepatit B hastalarında histolojik fibroziz evreleri ile glutatyon s-transferaz izozimlerinin expresyonlarının karşılaştırılması GSTPİ izoenziminin olası etkisini immünohistokimyasal boyama ile araştırdık. Yapılan çalışmada elli dokuz hepatit B dokusunda , GSTPİ (P1) izoziminin immünohistokimyasal bulguları değerlendirildi. Bu hastalara ait dokular boyanma şiddetine göre karşılaştırıldığında Hepatit B dokularının GSTPİ(P1) izoziminin protein ekspresyonunun viral hepatit karaciğer dokularında yüksek düzeyde expresyon göstermiştir.Bu bulgulara göre GSTP1 izoziminin viral Hepatit B hastalığının gidişatında önemli rol oynayabileceği gösterilmiştir. Anahtar kelimeler: Hepatit B, GSTP1, immünohistokimya
ABSTRACT COMPARISON OF THE EXPRESSIONS OF GLUTATIONE- S -TRANSFERASE ISOENZYMES WITH HISTOPATHOLOGICAL FIBROUS STAGES IN CHRONIC ACTIVE HEPATITIS B PATIENTS EROĞLU, Ergün Kırıkkale University Graduate School of Natural and Applied Sciences Department of Biology, Master Thesis Advisor: Prof. Dr. Serpil OĞUZTÜZÜN January 2020, 43 pages In this study, histological fibrosis stages and expression of glutathione S-transferase (GST) pi isoenzyme in chronic active hepatitis B patients were compared and the possible effect of GSTPi isoenzyme was investigated by immunohistochemical staining method. In the study, immunohistochemical findings of GSTPi (GSTP1) isoenzyme were evaluated from fifty-nine liver tissue of hepatitis B patients. When the tissues of these patients are compared according to the staining intensity, protein expression of the GSTP1 isozyme showed high levels of expression in the liver tissues of viral hepatitis B patients. According to these findings, it was shown that GSTP1 isozyme may play an important role in the development of viral hepatitis B disease. Key Words: Hepatit B, Liver tissue, GSTP1, immunohistochemistry
ABSTRACT COMPARISON OF THE EXPRESSIONS OF GLUTATIONE- S -TRANSFERASE ISOENZYMES WITH HISTOPATHOLOGICAL FIBROUS STAGES IN CHRONIC ACTIVE HEPATITIS B PATIENTS EROĞLU, Ergün Kırıkkale University Graduate School of Natural and Applied Sciences Department of Biology, Master Thesis Advisor: Prof. Dr. Serpil OĞUZTÜZÜN January 2020, 43 pages In this study, histological fibrosis stages and expression of glutathione S-transferase (GST) pi isoenzyme in chronic active hepatitis B patients were compared and the possible effect of GSTPi isoenzyme was investigated by immunohistochemical staining method. In the study, immunohistochemical findings of GSTPi (GSTP1) isoenzyme were evaluated from fifty-nine liver tissue of hepatitis B patients. When the tissues of these patients are compared according to the staining intensity, protein expression of the GSTP1 isozyme showed high levels of expression in the liver tissues of viral hepatitis B patients. According to these findings, it was shown that GSTP1 isozyme may play an important role in the development of viral hepatitis B disease. Key Words: Hepatit B, Liver tissue, GSTP1, immunohistochemistry
Açıklama
Anahtar Kelimeler
Biyoloji, Biology
