Atların dışkılarından izole edilen Escherichia coli izolatlarında antimikrobiyal direnç ve genişlemiş spektrumlu beta laktamaz üretiminin fenotipik olarak araştırılması
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Dosyalar
Tarih
2013
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Kırıkkale Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada Ankara?daki Hipodrom ve Konkur atlarından alınan dışkı örneklerinden izole edilen E. coli suşlarının çeşitli antibiyotiklere direnç durumlarını belirlemek ve GSBL varlığını araştırmak amaçlanmıştır. Bu amaçla elde edilen 100 E. coli (konkur: 63, hipodrom: 37) izolatının 16 antibiyotiğe karşı duyarlılıkları Disk Difüzyon yönteminden yararlanarak, GSBL varlığı fenotipik olarak analiz edildi. Hipodrom grubu ve Konkur grubu arasında antibiyotik direnç prevalansı karşılaştırıldı.Çalışmada konkur tesisindeki atlardan izole edilen 63 adet E. coli izolatında en yüksek direnç tetrasikline %20.6 (13), Hipodromdaki atlardan izole edilen 37 adet E. coli izolatında da en yüksek direnç tetrasikline %81.1 (30) karşı tespit edildi. Toplam 100 attaki GSBL prevalansı %6 (6) olarak tespit edildi.
In this study, it is aimed to research the existance of ESBL and determine the resistance of E. coli strains isolated from feces examples obtained from horses located in Ankara Race Track and from show jumping horses in Ankara, against various antibiotics. In this aim, the sensitivity of 100 E. coli isolates are analysed against 16 antibiotics by using the Disc Diffusion method and also the ESBL existance has been analysed phenotypicly. The antibiotic resistance prevalence has been compared between race track group horses and show jumping group of horses. In this study, in 63 of E. coli isolates isolated from show jumping horses, the highest resistance against tetracycline is determined as 20.6% (13), whereas in 37 E. coli isolates isolated from race track horses, the highest resistance against tetracycline is determined as 81.1% (30). The total prevalance of ESBL in 100 horses has been determined as 6% (6).
In this study, it is aimed to research the existance of ESBL and determine the resistance of E. coli strains isolated from feces examples obtained from horses located in Ankara Race Track and from show jumping horses in Ankara, against various antibiotics. In this aim, the sensitivity of 100 E. coli isolates are analysed against 16 antibiotics by using the Disc Diffusion method and also the ESBL existance has been analysed phenotypicly. The antibiotic resistance prevalence has been compared between race track group horses and show jumping group of horses. In this study, in 63 of E. coli isolates isolated from show jumping horses, the highest resistance against tetracycline is determined as 20.6% (13), whereas in 37 E. coli isolates isolated from race track horses, the highest resistance against tetracycline is determined as 81.1% (30). The total prevalance of ESBL in 100 horses has been determined as 6% (6).
Açıklama
Anahtar Kelimeler
Veteriner Hekimliği, Veterinary Medicine
