Damızlık japon bıldırcın (coturnix coturnix japonica) rasyonlarına mannan-oligosakkarit ve ß-glukan ile vitamin C ilavesinin immunite ve antioksidan durum üzerine etkileri
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Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Kırıkkale Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışma, damızlık bıldırcın rasyonuna prebiyotik (Mannan – oligosakkarit -?-Glukan, ImmunoWallTM) ve vitamin C ilavesinin performans, bazı biyokimyasal parametreler, immunite ve antioksidan durum üzerine etkilerini belirlemek amacıyla yapıldı. Çalışmada 10 haftalık toplam 120 adet Japon bıldırcını (Coturnix coturnix japonica) kullanıldı. Hayvanlar, her birinde 6 (4 dişi+2 erkek) bıldırcın bulunan 5 tekrarlı 4 deneme grubuna ayrıldı. Deneme grupları 1. grup bazal rasyon (kontrol), 2. grup 4 g/kg prebiyotik (Mannan – oligosakkarit - ?-Glukan), 3.grup 300 mg/kg vitamin C, 4. grup 4 g/kg prebiyotik (Mannan – oligosakkarit -?-Glukan ) +300 mg/kg vitamin C olacak şekilde düzenlendi. Deneme 8 hafta sürdü. Çalışma başında ve sonunda bıldırcınların, canlı ağırlıkları (CA), canlı ağırlık artışları (CAA), yumurta verimi (YV), yumurta ağırlığı (YA), yumurta kitlesi (YK), yem tüketimleri (YT), yemden yararlanma oranları (YYO) belirlendi. Serumda glikoz, total kolesterol, HDL-kolesterol, LDL-kolesterol, trigliserid, total protein, albümin, immunglobulin A (IgA), immunglobulin G (IgG), immunglobulin M (IgM) düzeyleri, plazmada malondialdehit (MDA), total antioksidan kapasite (TAK), total oksidan kapasite TOK, nitrik oksit (NO) düzeyleri, glutasyon peroksidaz (GSH-Px), süperoksit dismutaz (SOD) , katalaz (CAT) aktiviteleri ölçüldü. Kontrol grubuna göre tüm deneme gruplarında CA, CAA, YA, YT, YYO'larında önemli değişiklikler görülmedi. Yumurta verimi prebiyotik verilen gruba göre prebiyotik ile vitamin C ilave edilen grupta arttı (P<0.05). Yumurta kitlesi kontrol grubuna göre prebiyotik ile vitamin C'nin kombine olarak ilave edildiği grupta anlamlı olarak artış gösterdi (P<0.05). Kontrol grubuna göre tüm deneme gruplarında serum glikoz, trigliserid, total kolesterol, HDL-kolesterol, LDL-kolesterol, total protein, albümin ve IgG düzeylerinde istatistiksel olarak fark bulunmadı (P>0.05). Prebiyotik ve vitamin C verilen tüm deneme grupların serum IgA düzeylerinin kontrol grubuna göre önemli düzeyde yüksek olduğu saptandı (P<0.001). Prebiyotik + vitamin C verilen grupta prebiyotik verilen gruba göre IgA düzeyleri yüksek bulundu. Kontrol grubunda serum IgM düzeyleri tüm deneme gruplarına göre önemli düzeyde yüksekti. Prebiyotik ve vitamin C kombine verilen grupta serum IgM düzeyleri diğer gruplara göre önemli düzeyde yüksekti Prebiyotik verilen grupta vitamin C verilen gruba göre IgM düzeyleri yüksekti (P<0.001). Kontrol grubuna göre tüm deneme gruplarında plazma MDA, TOK, NO düzeylerinde, SOD, CAT, GSH-Px aktivitelerinde istatistiksel olarak fark bulunmadı. Plazma TAK ve vitamin C düzeyleri kontrol grubuna göre deneme gruplarında yüksek bulundu (P<0.001). Sonuç olarak, kanatlılarda MOS-?-glukan ve vitamin C' nin özellikle kombine şekilde performans, biyokimyasal ve immünolojik parametreler ile antioksidan durum üzerinde etkilerinin tam olarak belirlenebilmesi için bu maddelerin farklı formlarını içeren preparat ve dozlarının kullanılabileceği yeni çalışmalar yapılabilir. Ayrıca, standart çevre koşullarında yetiştirilen kanatlıların rasyonlarına vitamin C ilavesini incelemek ve daha fazla araştırma yapmak gerekmektedir.
In this study, the effects of prebiotic (mannan-oligosaccharides and ?-glucan, ImmunoWallTM) and vitamin C supplementation alone and in combination in ration on some biochemical parameters, immunity and anti-oxidant effects were investigated in breeding Japon quail (Coturnix coturnix japonica). A total of 120, 10 weeks old, Japanese quails were used in this study. Quails were assigned to four groups with 5 replicates of 6 (4 females, 2 males) quails each. Treatment for each group consisted of: first group (control group) received basal diet without supplementation; second group received 4 g/kg prebiotic (mannan-oligosaccharides -?-glucan, ImmunoWallTM); third group received 300 mg/kg vitamin C; and fourth group received 4 g/kg prebiotic (mannan-oligosaccharides-?-glucan, ImmunoWallTM) + 300 mg/kg vitamin C. The experiment lasted 8 weeks. At the beginning and at the end of the study, live-weights (LW), live-weight gain (LWG), egg yield (EY), egg weights (EW), egg mass (EM), feed consumption (FC), feed conversion rate (FCR) of the quails were determined at the beginning and end of the study.Serum glucose, triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol, total protein, albumin, immunoglobulin A (IgA), immunoglobulin A immunoglobulin G (IgG), immunoglobulin M (IgM), plasma levels of malondialdehyde (MDA), total oxidant capacity (TOC), total antioxidant capacity (TAC), nitric oxide (NO), glutathione peroxidase (GSH-PX) superoxide dismutase (SOD), catalase (CAT) were determined. Comparing the experimental groups to the control group, no significant differences were observed in terms of LW, LWG, EW, FC and FCR. An increment in EY was observed in the group supplemented with MOS-?-glucan and Vitamin C as compared to the MOS-?-glucan supplemented group (P<0.05). Again, the group supplemented with MOS-?-glucan and Vitamin C showed a meaningful increment in EM as compared to the control group (P<0.05). The experimental groups did not show any significant statistical differences with regards to serum glucose, triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol, total protein, albumin, IgG levels when compared to the control group (P>0.05). However, levels of serum IgA were observed to be higher in groups supplemented with prebiotic and vitamin C as compared to the control group (P<0.001). Immunglobulin A levels were found to be higher in the group given prebiotic + vitamin C and compared to the group given prebiotic (P<0.001). The control group had a higher level of serum IgM than all the experimental groups. Furthermore, the group supplemented with prebiotic and vitamin C had a higher serum IgM level than the other groups (P<0.001). Prebiotic supplemented group also had a higher serum IgM level than vitamin C supplemented group. There was no statistically significant difference in plasma MDA, TOC, NO levels, SOD, CAT, GSH-Px activities in all experimental groups compared to the control group. Nonetheless, plasma levels of TAS and vitamin C were observed to be higher in experimental groups than the control group (P<0.001). As a result, new studies can be conducted in which preparations and doses containing different forms of these substances especially in combined form can be used in order to fully determine the effects of MOS+?-glucan and vitamin C on performance, biochemical parameters, immunological parameters and antioxidant status in poultry. In addition, it is necessary to examine the addition of vitamin C to the diets of poultry reared under standard environmental conditions and to do more research.
In this study, the effects of prebiotic (mannan-oligosaccharides and ?-glucan, ImmunoWallTM) and vitamin C supplementation alone and in combination in ration on some biochemical parameters, immunity and anti-oxidant effects were investigated in breeding Japon quail (Coturnix coturnix japonica). A total of 120, 10 weeks old, Japanese quails were used in this study. Quails were assigned to four groups with 5 replicates of 6 (4 females, 2 males) quails each. Treatment for each group consisted of: first group (control group) received basal diet without supplementation; second group received 4 g/kg prebiotic (mannan-oligosaccharides -?-glucan, ImmunoWallTM); third group received 300 mg/kg vitamin C; and fourth group received 4 g/kg prebiotic (mannan-oligosaccharides-?-glucan, ImmunoWallTM) + 300 mg/kg vitamin C. The experiment lasted 8 weeks. At the beginning and at the end of the study, live-weights (LW), live-weight gain (LWG), egg yield (EY), egg weights (EW), egg mass (EM), feed consumption (FC), feed conversion rate (FCR) of the quails were determined at the beginning and end of the study.Serum glucose, triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol, total protein, albumin, immunoglobulin A (IgA), immunoglobulin A immunoglobulin G (IgG), immunoglobulin M (IgM), plasma levels of malondialdehyde (MDA), total oxidant capacity (TOC), total antioxidant capacity (TAC), nitric oxide (NO), glutathione peroxidase (GSH-PX) superoxide dismutase (SOD), catalase (CAT) were determined. Comparing the experimental groups to the control group, no significant differences were observed in terms of LW, LWG, EW, FC and FCR. An increment in EY was observed in the group supplemented with MOS-?-glucan and Vitamin C as compared to the MOS-?-glucan supplemented group (P<0.05). Again, the group supplemented with MOS-?-glucan and Vitamin C showed a meaningful increment in EM as compared to the control group (P<0.05). The experimental groups did not show any significant statistical differences with regards to serum glucose, triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol, total protein, albumin, IgG levels when compared to the control group (P>0.05). However, levels of serum IgA were observed to be higher in groups supplemented with prebiotic and vitamin C as compared to the control group (P<0.001). Immunglobulin A levels were found to be higher in the group given prebiotic + vitamin C and compared to the group given prebiotic (P<0.001). The control group had a higher level of serum IgM than all the experimental groups. Furthermore, the group supplemented with prebiotic and vitamin C had a higher serum IgM level than the other groups (P<0.001). Prebiotic supplemented group also had a higher serum IgM level than vitamin C supplemented group. There was no statistically significant difference in plasma MDA, TOC, NO levels, SOD, CAT, GSH-Px activities in all experimental groups compared to the control group. Nonetheless, plasma levels of TAS and vitamin C were observed to be higher in experimental groups than the control group (P<0.001). As a result, new studies can be conducted in which preparations and doses containing different forms of these substances especially in combined form can be used in order to fully determine the effects of MOS+?-glucan and vitamin C on performance, biochemical parameters, immunological parameters and antioxidant status in poultry. In addition, it is necessary to examine the addition of vitamin C to the diets of poultry reared under standard environmental conditions and to do more research.
Açıklama
Sağlık Bilimleri Enstitüsü, Biyokimya (Veterinerlik) Ana Bilim Dalı, Biyokimya Bilim Dalı
Anahtar Kelimeler
Biyokimya, Biochemistry
