Deri kanserinde GST izozimlerinin ekspresyonlarının rolü
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Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Kırıkkale Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Deri Kanseri çeşitli genetik ve çevresel faktörlerin (ultraviyole (UV) ışınlarına maruz kalma, sigara içme, genetik yatkınlılık ve bağışıklık sistemi sorunları gibi) etkileşimiyle oluşan serbest radikallerin neden olduğu oksidatif stresle tetiklenebilir. Glutatyon S-Transferazlar (GST'ler), hücreyi kimyasal kanserojenlere, toksik kimyasallara ve reaktif oksijen türlerine karşı koruyan detoksifiye edici enzimlerin bir ailesidir. GST enzimleri, serbest radikallerin neden olduğu zararlı bileşikleri nötralize ederek DNA hasarını azaltmaya yardımcı olur. Böylece, GST enzimlerinin yeterli seviyede bulunması, deri kanserinin önlenmesinde ve hücrelerin hasardan korunmasında önemli bir rol oynar. GST pi (GSTP) bu enzim sisteminin önemli üyesidir. Bu çalışmada, 62 deri tümörü ve normal dokuda GSTP izozimi ekspresyonunu incelemeyi amaçladık. Tümör dokularında GSTP ekspresyonu normal dokulardan yüksek olmakla birlikte, gruplar arasında GSTP ekspresyonu bakımından anlamlı bir farklılık bulunmamıştır (p>0.05). Hastaların yaşı ile tümör boyutu arasında da pozitif yönlü ve anlamlı korelasyon gözlenmiştir (p=0,040; p<0,05). Erkek hastalardan alınan normal ve tümör dokularındaki ekspresyon düzeyinin kadın hastalara göre daha yüksek olduğu görülmüştür. Orta derece diferansiye tömörlerin GSTP ekspresyonunun iyi derece diferansiye tümörlerden yüksek olduğu görülmüştür. Skuamöz hücreli karsinom dokularının ortalama GSTP ekspresyonunun bazal hücrelilere göre daha yüksek olduğu görülmüştür. Tümör evresi ve boyutuna göre değerlendirme yapıldığında en yüksek ekspresyon T2 evre ile 2-4 cm boyutundaki dokularda bulunmuştur. Sonuç olarak, deri tümörü dokularında GSTP'nin yüksek ekspresyon göstermesi, hastalığın patogenezi ve progresyonunda bu parametrelerin önemli rolü olabileceğini göstermektedir. Hem kanserin önlenmesinde hem de tedavisinde GST enzimlerinin etkinliği, oksidatif stresle mücadele ederek hücrelerin korunmasına yardımcı olur. Anahtar Kelimeler: Deri kanseri, Glutatyon S-Transferaz pi, immunohistokimya
Skin Cancer is triggered by oxidative stress caused by free radicals, which are formed by the interaction of various genetic and environmental factors (such as exposure to ultraviolet (UV) rays, smoking, genetic predisposition and immune system problems). Glutathione S-transferases (GSTs) are a family of detoxifying enzymes that protect the cell against chemical carcinogens, toxic chemicals and reactive oxygen species. GST enzymes help reduce DNA damage by neutralizing harmful compounds caused by free radicals. Thus, adequate levels of GST enzymes play an important role in preventing skin cancer and protecting cells from damage. GST pi (GSTP) is an important member of this enzyme system. In this study, we aimed to examine the expression of GSTP isozyme in 62 skin tumors and normal tissues. Although GSTP expression was higher in tumor tissues than in normal tissues, no significant difference was found between groups in terms of GSTP expression (p>0.05). A positive and significant correlation was also observed between the age of the patients and the tumor size (p=0.040; p<0.05). It was observed that the expression level in normal and tumor tissues from male patients was higher than in female patients. Moderately differentiated tumors showed higher GSTP expression than well-differentiated tumors. It was observed that the mean GSTP expression of squamous cell carcinoma tissues was higher than basal cell carcinomas. When evaluated according to tumor stage and size, the highest expression was found in tissues with T2 stage and 2-4 cm in size. In conclusion, the high expression of GSTP in skin tumor tissues indicates that these parameters may have an important role in the pathogenesis and progression of the disease. The effectiveness of GST enzymes in both cancer prevention and treatment helps protect cells by combating oxidative stress. Keywords: Skin cancer, Glutathione S-Transferase pi, immunohistochemistry
Skin Cancer is triggered by oxidative stress caused by free radicals, which are formed by the interaction of various genetic and environmental factors (such as exposure to ultraviolet (UV) rays, smoking, genetic predisposition and immune system problems). Glutathione S-transferases (GSTs) are a family of detoxifying enzymes that protect the cell against chemical carcinogens, toxic chemicals and reactive oxygen species. GST enzymes help reduce DNA damage by neutralizing harmful compounds caused by free radicals. Thus, adequate levels of GST enzymes play an important role in preventing skin cancer and protecting cells from damage. GST pi (GSTP) is an important member of this enzyme system. In this study, we aimed to examine the expression of GSTP isozyme in 62 skin tumors and normal tissues. Although GSTP expression was higher in tumor tissues than in normal tissues, no significant difference was found between groups in terms of GSTP expression (p>0.05). A positive and significant correlation was also observed between the age of the patients and the tumor size (p=0.040; p<0.05). It was observed that the expression level in normal and tumor tissues from male patients was higher than in female patients. Moderately differentiated tumors showed higher GSTP expression than well-differentiated tumors. It was observed that the mean GSTP expression of squamous cell carcinoma tissues was higher than basal cell carcinomas. When evaluated according to tumor stage and size, the highest expression was found in tissues with T2 stage and 2-4 cm in size. In conclusion, the high expression of GSTP in skin tumor tissues indicates that these parameters may have an important role in the pathogenesis and progression of the disease. The effectiveness of GST enzymes in both cancer prevention and treatment helps protect cells by combating oxidative stress. Keywords: Skin cancer, Glutathione S-Transferase pi, immunohistochemistry
Açıklama
Fen Bilimleri Enstitüsü, Fen Bilimleri Ana Bilim Dalı, Biyoloji Bilim Dalı
Anahtar Kelimeler
Biyoloji, Biology
