Kırıkkale bölgesinde koyun kökenli Echinococcus granulosus izolatlarının moleküler karakteri
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Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, koyunlardan elde edilen Echinococcus granulosus’un moleküler karakterizasyonunu belirlemek amaçlandı. Bunun için enfekte koyunların değişik organlarından elde edilen 24 adet kist hidatit’e ait germinal membran ve protoskoleks örneklerinden DNA izolasyonu yapıldı ve daha sonra DNA örnekleri 16 primer kullanılarak RAPD-PCR metodu ile tarandı. Echinococcus granulosus G1 suşu referans materyali olarak kullanıldı. Kullanılan 16 primerden 4’ü (AP2, AP4, OPB12 ve G05) koyun izolatları ve referans G1suşunun her ikisinde de tipik RAPD band kalıpları verdi. AP2 primeri ile oluşan 250 baz çiftlik tek bant koyun izolatları ve G1 suşunun her ikisinde de ortaktı. Sonuçlar bu çalışmada örneklenen koyun izolatlarının hepsinin aynı Echinococcus granulosus suşu olacağına işaret etmektedir.
In present study, it was aimed to determine molecular characterization of Echinococcus granulosus obtained from sheep. For this purpose, DNA isolation was done from germinal membrans and protoscolices of 24 hydatic cyst samples obtained from different organs of infected sheep and then DNA samples were scanned by RAPD-PCR using 16 primers. Echinococcus granulosus G1 strain was used as reference material. Only 4 of these 16 primers (AP2, AP4, OPB12 and G05) gave the typical RAPD band patterns in both sheep isolates and the reference G1 strain. The single band of 250 bp occurred by AP2 primer was common in both sheep isolates and G1strain According to result, AP2 primer can be successfully used for separation of G1 strain in different isolates. The results indicated that all sheep isolates sampled in this study can be assumed in the same Echinococcus granulosus strain.
In present study, it was aimed to determine molecular characterization of Echinococcus granulosus obtained from sheep. For this purpose, DNA isolation was done from germinal membrans and protoscolices of 24 hydatic cyst samples obtained from different organs of infected sheep and then DNA samples were scanned by RAPD-PCR using 16 primers. Echinococcus granulosus G1 strain was used as reference material. Only 4 of these 16 primers (AP2, AP4, OPB12 and G05) gave the typical RAPD band patterns in both sheep isolates and the reference G1 strain. The single band of 250 bp occurred by AP2 primer was common in both sheep isolates and G1strain According to result, AP2 primer can be successfully used for separation of G1 strain in different isolates. The results indicated that all sheep isolates sampled in this study can be assumed in the same Echinococcus granulosus strain.
Açıklama
Anahtar Kelimeler
Veterinerlik
Kaynak
Kafkas Üniversitesi Veteriner Fakültesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
16
Sayı
2
