İnsan ve sığırlardan izole edilen fekal Escherichia coli suşlarında antibiyotik direnci ve genişlemiş spektrumlu beta-laktamaz üretimi
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Tarih
2005
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info:eu-repo/semantics/openAccess
Özet
Son bir ay içinde antibiyotik kullanmamış insanların dışkılarından izole edilen herbiri farklı bireye ait 140 Escherichia coli susu ile sığırların rektum mukozasından izole edilen 114 E.coli susunun 10 antibiyotiğe duyarlılığı disk difüzyon yöntemi ile, bu suşlarda genişlemiş spektrumlu beta-laktamaz (GSBL) üretimi fenotipik doğrulama testi ile belirlenmiştir. İnsan suşlannda ampisilin, ampisilin-sulbaktam, amoksisilin-klavulanik asit, piperasilin-tazobaktam, sefotaksim, siprofloksasin, trimetoprim-sulfametoksazol direnci sığır suşlarındakinden, sığır suşlannda amikasin direnci insan suşlanndakinden anlamlı derecede daha yüksek bulunmuştur. Seftazidim ve gentamisin direnci insan suşlannda daha yüksek bulunmuşsa da fark anlamlı değildir. İki grup susta da en yüksek direnç ampisilin için bulunmuştur. İnsan suşlannda direnç cinsiyete göre fark göstermemiş, ancak 13 yaş ve altındaki çocuklara ait suşlarda büyüklerden izole edilenlere göre ampisilin, ampisilin-sulbaktam, seftazidim, gentamisin ve trimetoprim-sulfametoksazol direnci anlamlı derecede yüksek bulunmuştur. İnsan suşlannda GSBL üretimi % 3 olarak saptanmış, sığır suşlannda GSBL üretimine rastlanmamıştır.
A hundred forty E.coli strains isolated from stools of humans who did not use antibiotics in the last one month and 114 E.coli strains isolated from cattle's rectal mucosa were enrolled in this study. Each strain was from different individuals. Susceptibility of these strains to ten antibiotics were determined by disk diffusion test and extended spectrum beta-lactamase (ESBL) production by phenotypic confirmation test. The resistance to ampicillin, ampicillin-sulbactam, amoxicilin-clavulanic asid, piperacillin-tazobactam, sefotaxime, ciprofloxacin and trimethoprim-sulfamethoxazole in human E. coli strains were found significantly higher than the resistance of cattle E. coli strains and the resistance to amikacin in cattle E. coli strains was found significantly higher than the resistance of human E. coli strains. Although the resistance to ceftazidime and gentamicin in human E.coli strains were found higher, the differences was not statistically significant. The highest resistance was for ampicillin in both cattle and human strains. The differences in antibiotic resistance of human strains were insignificant in two genders. But the antibiotic resistance were significantly higher in the strains from children under 13 age than the strains from adults for ampicillin, ampicillin-sulbactam, trimetoprim-sulfamethoxazole, gentamisin and ceftazidime. While ESBL production from human strains was 3 %, there was no production in cattle strains.
A hundred forty E.coli strains isolated from stools of humans who did not use antibiotics in the last one month and 114 E.coli strains isolated from cattle's rectal mucosa were enrolled in this study. Each strain was from different individuals. Susceptibility of these strains to ten antibiotics were determined by disk diffusion test and extended spectrum beta-lactamase (ESBL) production by phenotypic confirmation test. The resistance to ampicillin, ampicillin-sulbactam, amoxicilin-clavulanic asid, piperacillin-tazobactam, sefotaxime, ciprofloxacin and trimethoprim-sulfamethoxazole in human E. coli strains were found significantly higher than the resistance of cattle E. coli strains and the resistance to amikacin in cattle E. coli strains was found significantly higher than the resistance of human E. coli strains. Although the resistance to ceftazidime and gentamicin in human E.coli strains were found higher, the differences was not statistically significant. The highest resistance was for ampicillin in both cattle and human strains. The differences in antibiotic resistance of human strains were insignificant in two genders. But the antibiotic resistance were significantly higher in the strains from children under 13 age than the strains from adults for ampicillin, ampicillin-sulbactam, trimetoprim-sulfamethoxazole, gentamisin and ceftazidime. While ESBL production from human strains was 3 %, there was no production in cattle strains.
Açıklama
Anahtar Kelimeler
Farmakoloji ve Eczacılık
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Cilt
19
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3
