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Öğe EVALUATION OF ESTHETIC SELF-PERCEPTIONS OF UNDER AND POST-GRADUATE DENTAL STUDENTS(2020) Çelik, Çiğdem; Adışen, Mehmet Zahit; Azaklı, Elif; Aktürk, Ömer; Kaçar, AyşenurBackground and Aim: This study aimed to evaluate the dentalestheticself-perceptionsofundergraduateandpostgraduate students attending the Faculty of Dentistry at Kırıkkale University. Materials and Methods:Aquestionnaireconsistingoftwo parts was applied to the participants. In the first part, the undergraduate and postgraduate participants answered a questionnaire measuring their self-perceptions of dental esthetics. In the second part, the students were asked to rate the importance of the characteristic features of a face on a 100-mm visual analog scale (VAS) bar. Data were statistically analyzed using the Pearson chi-square, Mann-Whitney U, and Kruskal-Wallis tests at a significance level of 0.05. Results: The overall mean satisfaction index was 6.86. The participants in the postgraduate group and female students were the most satisfied with their smiles (p<0.05). Among the facial features important to facial attractiveness, teeth scored the highest (86.9) on the VAS. The female students achieved an overall higher mean score than the males on the VAS for facial features: teeth, nose, and lips (p<0.05). Conclusion: The dental students in the study group were found to have a moderate degree of satisfaction with their individual dental esthetic characteristics, and this phenomenon varied because of gender and academic level.Öğe Evaluation of photochemically cross-linked collagen/gold nanoparticle composites as potential skin tissue scaffolds(Tubitak Scientific & Technological Research Council Turkey, 2023) Yelkuvan, Evrim Meriç; Erdemli, Özge; Yılmaz, Bengi; Aktürk, ÖmerCollagen type I is the main structural unit in skin tissue and is therefore used preferentially in skin tissue scaffolds. However, collagen-based 3D scaffolds have weak aqueous stability and degradation profiles in their uncross-linked states and chemical cross -linking reagents arise toxicity concerns, which generally restrict the spectrum of their biomedical applicability. Here, the research goal is to photochemically cross-link collagen type I with rose bengal (RB) when subjected to green laser light and to investigate the effect of silk sericin-capped gold nanoparticles (S-AuNP) when incorporated into scaffolds on the cross-linking process and thus on the scaffold properties. All the collagen scaffolds, that is plain collagen (C), collagen/S-AuNP (C-Au), cross-linked collagen (C-RBL), and cross-linked collagen/S-AuNP (C-AuRBL) were characterized for their potential as skin tissue scaffolds. C-AuRBL group had the best thermal stability, resistance to enzymatic degradation, and more uniform pore size distribution. None of the groups had cytotoxicity (cell viability > 70%) regarding the microscopic observations and MTT cell viability assays for L929 fibroblasts. L929 fibroblasts and primary adult human epidermal keratinocytes (HEKa) were also separately seeded on C-AuRBL scaffolds and according to microscopy results, they could support the stimulation of adhesion, morphological changes, and spreading of both cells, thereby encouraging the usage of this fabrication strategy for prospective skin tissue scaffolds.Öğe Lazer Ablasyonu ile Sentezlenmiş Levan Kaplı Altın Nanoparçacıkların siRNA Transfeksiyon Ajanı Olma Potansiyeli Üstüne Çalışma(Kırıkkale Üniversitesi, 2022) Aktürk, Ömer; Çağdaş Tunalı, BesteBu çalışmada, altın nanoparçacık (AuNP) temelli kısa interferanslı RNA (siRNA) transfeksiyon vektörü tasarlanmıştır. Sıvı içinde atımlı lazer ablasyon yöntemiyle (PLAL) levan polisakkariti içeren ortamda sentezlenen AuNP’ler (Levan-AuNP), karakterizasyon testlerinin ardından MCF-7 meme kanseri hücrelerinde siRNA transfeksiyon çalışmalarında kullanılmıştır. Hedefsiz siRNA sekansı ile gerçekleşen %48’lik Levan-AuNP-siRNA etkileşimi A260 absorbans ölçümleriyle bulunmuştur. Farklı derişimlerde Levan-AuNP ve 50 nM siGLO Green transfeksiyon indikatörü hazırlanarak MCF-7 hücrelerindeki optimum siRNA transfeksiyon koşulları belirlenmiştir. Transfeksiyon çalışmalarında öncelikle üçlü floresan boyama (siGLO Green, propidyum iyodür (PI) ve Hoechst 33342) yapılmış ve sitotoksisite görülmemiştir. Özellikle en yüksek derişimdeki Levan-AuNP grubunda ilk günde transfeksiyon gerçekleşirken, tek başına siGLO Green grubunda transfeksiyon gerçekleşmemiştir. 2 ve 4 günlük inkübasyonda ise tüm gruplarda transfeksiyon gözlemlenmiştir. PI ve Hoechst kullanılmadan yapılan ikinci transfeksiyon çalışmalarında ise siGLO Green floresan şiddetinin belirgin şekilde arttığı ve özellikle Levan-AuNP-siGLO Green komplekslerinde daha kuvvetli ve noktalı bir floresan ışıma görülmüştür. Ortalama floresan şiddetleri ölçümlerine göre, tüm Levan-AuNP gruplarında istatistiksel açıdan anlamlı ölçüde artış bulunmuştur. Sonuç olarak, bu çalışmada geliştirilen Levan-AuNP’nin, mevcut transfeksiyon ajanlarına alternatif olabileceği gösterilmiştir.Öğe Synthesis and characterization of polyethyleneimine/silk fibroin/gold nanoparticle nanocomposites: Potential application as a gene carrier in breast cancer cell lines(Pergamon-Elsevier Science Ltd, 2023) Tunalı, Beste Çağdaş; Aktürk, Ömer; Şahingöz, Durukan; Türk, Mustafa; Keskin, Ayten ÇelebiThere is a need in advancing the design of non-viral gene carriers, thus herein using the pulsed laser ablation in liquid (PLAL) technique, polyethyleneimine-capped gold nanoparticles (PEI-AuNPs) were synthesized and then combined with Bombyx mori silk fibroin (SF) to form the nanocomposites (PEI-SF-AuNPs). After the characterization analyses were carried out, PEI-AuNPs and PEI-SF-AuNPs were tested for their in vitro biocompatibility in MCF-7 and MDA-MB231 breast cancer cells. Finally, the transfection capability of the selected group was observed. PEI capping enabled the cationic modification of the AuNP surface and SF reduced the surface charge (from 35 to 4 mV). PEI-AuNPs were highly monodispersed in shape and size (8-9 nm). The nanoparticle group synthesized in 5% (w/v) PEI concentration (PEI-AuNP5) had increased cytotoxicity as compared to the group synthesized in 1% (w/v) PEI concentration (PEI-AuNP1), but the conjugation of SF with PEI-AuNPs alleviated this cytotoxic tendency significantly. The group (PEI-AuNP1) with the most negligible cytotoxicity proved to be successful in the transfection of siRNA analog, i.e., siGLO green transfection indicator. Overall, the PEI-AuNP1 nanocomposites could be suggested as potent siRNA carriers due to the above beneficial properties in genebased breast cancer therapies.Öğe The anticancer activity of doxorubicin-loaded levan-functionalized gold nanoparticles synthesized by laser ablation(Elsevier, 2022) Aktürk, ÖmerHere, gold nanoparticles (AuNPs) were synthesized upon exposure to nano-pulsed Nd-YAG laser irradiation in de-ionized water (PLAL) and functionalized with levan polysaccharide for assessing the anticancer efficacy of doxorubicin (DOX)-conjugated levan-capped AuNPs complexes to MCF-7 breast cancer cells. According to the physicochemical test results, the increments in levan amount enhanced the colloidal stability and the drug encapsulation efficiency (DEE) significantly. For the 10L-AuNP group having the highest levan amount (10 mg/ mL levan), DEE was calculated as 92.21 +/- 0.56%. The lean levan, uncapped AuNPs, and 10L-AuNP were found non-cytotoxic (> 80% cell viability) in the studied concentrations with 48 h MTT assays. At higher DOX loadings (25, 50, and 100 mu g/mL) of 10L-AuNP, the cell viability reduced significantly compared to free DOX. Overall, these nanoparticle complexes could be proposed as potent drug delivery vehicles for cancer drugs such as DOX, as well as other drugs in the prospective studies.
