The anticancer activity of doxorubicin-loaded levan-functionalized gold nanoparticles synthesized by laser ablation
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Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Here, gold nanoparticles (AuNPs) were synthesized upon exposure to nano-pulsed Nd-YAG laser irradiation in de-ionized water (PLAL) and functionalized with levan polysaccharide for assessing the anticancer efficacy of doxorubicin (DOX)-conjugated levan-capped AuNPs complexes to MCF-7 breast cancer cells. According to the physicochemical test results, the increments in levan amount enhanced the colloidal stability and the drug encapsulation efficiency (DEE) significantly. For the 10L-AuNP group having the highest levan amount (10 mg/ mL levan), DEE was calculated as 92.21 +/- 0.56%. The lean levan, uncapped AuNPs, and 10L-AuNP were found non-cytotoxic (> 80% cell viability) in the studied concentrations with 48 h MTT assays. At higher DOX loadings (25, 50, and 100 mu g/mL) of 10L-AuNP, the cell viability reduced significantly compared to free DOX. Overall, these nanoparticle complexes could be proposed as potent drug delivery vehicles for cancer drugs such as DOX, as well as other drugs in the prospective studies.
Açıklama
Anahtar Kelimeler
Levan; Gold nanoparticle; Doxorubicin; Nano-pulsed laser; Cytotoxicity; Anticancer activity
Kaynak
International Journal of Biological Macromolecules
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
196