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Öğe Accidental ingestion of a pen in an adolescent girl(Edizioni Minerva Medica, 2016) Sapmaz, Ferdane; Aslan, Mustafa Kemal; Guenal, Yasemin Dere; Boybeyi, Oezlem; Kalkan, Ismail Hakki; Guliter, SefaForeign body ingestion is a common problem in pediatric population with a peak incidence between 6 months and 6 years. adolescents, however, are more likely to swallow or insert foreign bodies intentionally as a risk-taking behavior, a bid for attention, or under the influence of drugs or alcohol. in this paper we report the case of an adolescent girl who accidentally ingested a pen, which was successfully removed with endoscopy.Öğe Bacterial adhesion to braided surgical sutures: an in vitro study(Springer, 2016) Boybeyi, Ozlem; Kacmaz, Birgul; Gunal, Yasemin Dere; Gul, Serdar; Yorubulut, Serap; Aslan, Mustafa KemalBackground Surgical suture materials are accepted to be associated with a substantial proportion of surgical site infections. These infections are related with biofilm formation similar to that of other synthetic and implantable medical devices. Methods We conducted an in vitro study to investigate the bacterial adherence to different types of braided surgical sutures. The included sutures were polyglactin (Vicryl (R)) group (VG), rapidly absorbable polyglactin (Rapide-Vicryl (R)) group (RVG), nitrofurazone-coated polyglactin (Vicryl (R)) group (FVG), polyethylene terephthalate (Etibond (R)) group (EG), and natural silk (Silk (R)) group (SG). All sutures were cut in 1 cm length, embedded into tryptic soy broth, and then 10(6)-CFU/ml Escherichia coli and Staphylococcus aureus were added. After the 24th and 96th hour of incubation, bacterial colonies were counted, and results were expressed as CFU/cm. Results E. coli adhesion was significantly lower in VG and significantly higher in SG compared to FVG, RVG, and EG at the 24th and 96th hour of cultivation (p< 0.05). The S. aureus adhesion results at 24th hour showed that VG had the least bacterial adhesion, and FVG had the most bacterial adhesion compared to other sutures (p< 0.05). The S. aureus adhesion results at the 96th hour of cultivation showed that bacterial adhesion on sutures was not significantly different between groups (p> 0.05). Conclusion Of all braided surgical sutures, bacterial adhesion is significantly lower in polyglactin and significantly higher in silk sutures. Nitrofurazone coverage of suture worsens S. aureus contamination of the suture.Öğe Caudate lobe of the liver as the only content of the umbilical cord hernia(Wiley-Blackwell, 2015) Boybeyi, Ozlem; Ozmen, Ismail; Gunal, Yasemin Dere; Aslan, Mustafa Kemal; Aliefendioglu, Didem…Öğe A comparison of dysfunctional voiding scores between patients with nocturnal enuresis and healthy children(Tubitak Scientific & Technical Research Council Turkey, 2014) Boybeyi, Ozlem; Aslan, Mustafa Kemal; Durmus, Emine Gul; Ozmen, Ismail; Soyer, TutkuBackground/aim: To compare dysfunctional voiding symptom scores (DVSSs) between enuretic children and nonenuretic controls and to investigate associated factors that may affect DVSS. Materials and methods: A questionnaire including demographic features, educational status of parents, DVSS questions, and urinary tract infection (UTI) history was designed. A total of 269 patients were included; Group 1 comprised 161 patients with no voiding symptoms and Group 2 comprised 108 patients with nocturnal enuresis (NE). Children with DVSS of greater than 8.5 were suspected to have dysfunctional voiding. The results were evaluated using SPSS 15.0 with Kruskal-Wallis and multivariate logistic regression tests. Results: The median DVSS was 2 (interquartile range [IQR]: 1-3) in Group 1 and 8 (IQR: 5-12) in Group 2. The percentage of children with DVSS greater than 8.5 was 0.6% in Group 1 and 53.1% in Group 2 (P = 0.01). The percentage of children with UTI history was significantly higher in Group 2 (34.3%) than Group 1 (15.9%) (P = 0.03). An increase in the educational level of the father decreased DVSS by 0.5-fold. Presence of UTI history increased DVSS 2.5-fold. Conclusion: The DVSS is a rapid, easy tool for determining abnormal voiding parameters in children. Children with NE had higher DVSSs, which was significantly affected by the father's educational status and the child's UTI history.Öğe Comparison of growth factor levels in patients with normal and hypospadiac prepuce(Tubitak Scientific & Technical Research Council Turkey, 2011) Soyer, Tutku; Ayva, Ebru Sebnem; Atasoy, Pinar; Aslan, Mustafa Kemal; Cakmak, Ahmet MuratAim: To compare the growth factor levels in patients with normal and hypospadiac prepuce. Materials and methods: Patients who underwent hypospadias repair were included in the study. The prepuce was excised after achieving successful hypospadias repair with satisfactory functional and cosmetic appearance. The patients who underwent preputial reconstructions and complicated repairs were excluded. The control group consists of boys without hypospadias. Elective circumcision was performed to have normal preputial samples. The VEGF levels, VEGF receptor (VEGFR) and TGF-beta receptor (TGF-beta-R) expressions were evaluated in preputial tissues by immunohistochemical analysis. Results: Fifteen patients were included in each group. The mean age of patients with normal and hypospadiac prepuce was 4.33 +/- 1.39 and 4.07 +/- 1.58, respectively (P>0.05). Although VEGF, VEGFR, and TGF-beta-R levels were decreased in hypospadiac prepuce, statistical significance was detected only in VEGF levels (P<0.05, Mann-Whitney U test). Conclusion: The decreased levels of VEGF obtained from patients with hypospadiac prepuce suggest a structural anomaly related with angiogenesis. This structural difference can be considered one of the factors causing impaired wound healing and recurrent complications after preputial reconstructions in hypospadias repair.Öğe Comparison of the effect of contrast medium, air, and ozone on ischemia/reperfusion injury due to experimental intussusception model(Tubitak Scientific & Technical Research Council Turkey, 2013) Senyucel, Mine Fedakar; Ayva, Ebru Sebnem; Aslan, Mustafa Kemal; Boybeyi, Ozlem; Soyer, Tutku; Aksoy, Nurkan; Cakmak, Ahmet MuratAim: To compare effects of contrast medium, air, and ozone on ischemia/reperfusion injury due to intussusception. Materials and methods: Thirty Wistar rats were allocated into five groups (n = 6). In the control group (CG), 2 cm of colonic segment, 5 cm distal to ileocecal valve, was removed. Intussusception was performed at 5 cm distal to the ileocecal valve by pushing a proximal intestinal segment distally by stile in experimental groups. One hour later, in the sham-operated group (SG), manual reduction was performed. In the reduction groups, reduction of intussusception was performed by contrast medium (CMG, 1/1 diluted omnipaqueR, infusion rate 2 mL/min), by air (AG, Pmax = 60 mmHg), or by ozone (OG, 10 mu g/mL, 0.5 mg/kg, Pmax = 60 mmHg). One hour after reduction, 2 cm of colonic segment was removed. Colonic tissues were evaluated for histopathological grades and oxidative injury markers (malondialdehyde [MDA], nitric oxide [NO], and total sulfhydryl [T-SH] values). Results: Mucosal injury score was significantly higher in AG then in CG or SG (P < 0.05). There was no difference regarding mucosal injury between the other groups (P > 0.05). MDA levels were significantly higher and NO levels were significantly lower in CMG, OG, and AG than in CG (P < 0.05). There was no difference between OG and the other experimental groups in both MDA and NO levels (P > 0.05). Conclusion: None of the reduction methods in intussusception have superiority over others in preventing ischemia/reperfusion injury.Öğe Dysfunctional voiding in children with asthma(Bmj Publishing Group, 2013) Soyer, Özge Uysal; Kılınçoğlu, Betül; Şenyücel, Mine Fedakar; Aslan, Mustafa Kemal; Çakmak, Murat; Dallar, Yıldız; Soyer, TutkuA prospective study was performed to evaluate the prevalence of suspected dysfunctional voiding (DV) and associated risk factors in children with asthma. The DV is defined as any voiding symptoms and/or urinary incontinence. Children (4-10-year-old) with asthma (n=178) and healthy subjects (n=197) were enrolled. DV and Incontinence Scoring System were administrated. Demographic features and asthma related characteristics were recorded. Suspected DV was noted in 19 (27.9%) of patients with asthma and 5 (6.6%) of healthy subjects in children younger than 6 years of age (p=0.001). In this patient group, asthma increased the risk of suspected DV (OR=5.7 (95% CI 1.988 to 16.344)). Children with asthma older than 6 years of age had similar prevalence of suspected DV but they had higher frequency of voiding and urgency. Asthma is associated with higher DV symptom scores in younger children who have already completed toilet training and with some of DV symptoms such as frequency and urgency in older children.Öğe The Effect of Body Mass Index on the Clinical Course of Appendicitis in Children(Ortadogu Ad Pres & Publ Co, 2012) Aslan, Mustafa Kemal; Cesur, Ozkan; Soyer, Tutku; Hancerliogullari, Oymen; Turkmen, Feyza; Cakmak, MuratObjective: A retrospective study was performed to evaluate the effect of body mass index (BMI) on the clinical course of appendicitis in children. Material and Methods: Patients between 6 and 18 years of age, who had undergone appendectomy in the last 2 years, were evaluated for age, sex, BMI, time from the beginning of complaints to diagnosis, acute Or perforated appendicitis, duration of hospitalization and complications retrospectively. BMI was evaluated according to percentiles developed for Turkish children between 6 and 18 years of age. Patients with percentiles between 10 to 75 were accepted as the normal BMI group, lower than 10 was the low BMI group and more than 75 was assessed as the high BMI group. Results: Ninety-six patients were included in the study. The median age was 13 (min: 6, max:16) in the normal BMI group [n=37, male/female (M/F)=1.84], 12(6-16) in the low BMI group (n=38, M/F=1.53) and 9(6-15) in the high BMI group (n=21, M/F=3.2). The acute/perforated appendicitis ratios were 3.1, 2.8 and 1.3, respectively in the normal, low and high BMI groups. There was no significant difference between the groups regarding the time from the beginning of complaints to diagnosis and duration of hospitalization (p>0.05). Although complication rates showed no difference between the normal and low BMI groups, the high BMI group had a higher incidence of complications compared to the normal and low BMI groups (p=0.04 and p=0.018,respectively). The follow-up period of the patients were 2 months to 2 years and the most common complication was wound infection. Conclusion: Children with high BMI have higher complication rates during the clinical course of appendicitis compared to children with low and normal BMI.Öğe The effect of colchicine and low-dose methotrexate on intestinal ischemia/reperfusion injury in an experimental model(W B Saunders Co-Elsevier Inc, 2014) Boybeyi, Ozlem; Gunal, Yasemin Dere; Atasoy, Pinar; Kisa, Ucler; Aslan, Mustafa KemalAim: Intestinal ischemia/reperfusion (I/R) injury is a serious clinical condition. Colchicine and low-dose methotrexate have anti-inflammatory features. An experimental model was conducted to investigate the effect of colchicine and methotrexate on intestinal I/R injury. Methods: Twenty-four rats were included. Only laparotomy was done in control group (CG, n = 6). In experimental groups, superior mesenteric artery was occluded. After 1 h ischemia, reperfusion (1 h) was started by de-occlusion. 30 min before reperfusion, saline in sham group (SG,n: 6), colchicine (1 mg/kg) in colchicine group (CNG, n: 6), andmethotrexate (0.1 mg/kg) in methotrexate group (MTXG, n: 6) were infused intraperitoneally. Small intestines were harvested for evaluation of intestinal mucosal injury (Chiu score) and oxidative stress markers (nitric oxide: NO, malondialdehyde: MDA, superoxide dismutase: SOD). Results: Biochemically, MDA levels were significantly low in CG compared to SG, CNG, and MTXG (p < 0.05). NO levels were significantly low and SOD levels were significantly high in CG compared to MTXG (p < 0.05). Histopathologically, Chiu score was significantly low in CG compared to SG, CNG, and MTXG (p < 0.05), and significantly high in MTXG compared to SG and CNG (p b 0.05). Conclusion: The present experimental model caused I/R injury in rat intestines. Contrary to literature, it was found that methotrexate worsens and colchicine does not attenuate intestinal I/R injury. (C) 2014 Elsevier Inc. All rights reserved.Öğe The effect of extracorporeal shock wave lithotripsy on distribution of interstitial cells of Cajal in rabbit renal pelvis and proximal ureter(Tubitak Scientific & Technical Research Council Turkey, 2015) Boybeyi, Ozlem; Fedakar Senyucel, Mine; Ayva, Ebru Sebnem; Soyer, Tutku; Aslan, Mustafa Kemal; Basar, Mehmet Murad; Cakmak, Ahmet MuratBackground/aim: An experimental study was performed to evaluate the effect of extracorporeal shock wave lithotripsy (ESWL) on the distribution of interstitial cells of Cajal (ICC) in rabbit renal pelvis and proximal ureter. Materials and methods: Six New Zealand rabbits were included. Right kidneys were exposed to a total of 3000 shock waves (14 kV) by using an electrohydraulic-type ESWL device. Right sides were allocated as the ESWL group (EG, n = 6) and left sides as the control group (CG, n = 6). Tissues were harvested on day 7. Tissues were examined histopathologically for the presence of edema, inflammation, congestion, hemorrhage, fibrosis, and vascularization. Mast cell tryptase and CD117 (c-kit) staining was performed for ICC distribution. Results: Although increased tissue edema in renal pelvises and increased inflammation in ureters were observed in EG, no statistical difference was detected between groups (P > 0.05). In CG, positive CD117 staining was detected in 2 renal pelvises and ureters. None of the EG samples showed CD117 staining and no statistical difference was detected between groups (P > 0.05). Conclusion: Rabbit does not appear to be a good model for investigating ICCs. ESWL may cause histopathological alterations in the renal pelvis and ureter. Since it has not been statistically proven, reduced contractility of the ureter after ESWL may not be attributed to altered distribution of ICCs in the renal pelvis and ureter.Öğe The Effect of Extractum Cepae, Heparin Sodium, and Allantoin in Experimental Peritoneal Adhesion(SPRINGER INDIA, 2020) Ozmen, Ismail; Gunal, Yasemin Dere; Atasoy, Pinar; Kisa, Ucler; Yorubulut, Serap; Aslan, Mustafa Kemal; Boybeyi-Turer, OzlemThe purpose of the study is to investigate the effects of extractum cepae (EC), heparin sodium (HS), and allantoin (AA) on postoperative peritoneal adhesion (PPA) prevention. Forty-eight rats were allocated into six groups (n = 8). PPA was performed by Harris method. In group 1, no additional procedure was done. Abdominal cavity was washed with normal saline in group 2, EC in group 3, HS in group 4, AA in group 5, and EC-HS-AA mixture (EHA-m) in group 6. On the 21st postoperative day, PPA was scored with Nair adhesion score and examined for fibrosis histopathologically and hydroxy-proline levels (OHP) biochemically. Macroscopically, PPA was decreased in groups 2, 4, and 6 compared to groups 1, 3, and 5 (p < 0.05) and decreased in group 2 compared to other groups (p < 0.05). Histopathologically, fibrosis was decreased in groups 2, 4, and 6 compared to other groups and decreased in group 2 compared to other groups (p < 0.05). Tissue OHP level was decreased in groups 2, 3, 4, and 5 compared to groups 1 and 6 (p < 0.05). Tissue OHP level was decreased in group 6 compared to group 1, but the difference was not statistically significant. PPA formation decreases with normal saline, HS and EHA-m, whereas increases with EC and AA both macroscopically and microscopically. Although HS and EHA-m seem to have adhesion prevention effect, washing the intestines with saline seems to be more effective.Öğe Effect of P/E-selectin blockage on antisperm antibody development and histopathological alterations in experimental orchitis(W B Saunders Co-Elsevier Inc, 2013) Cesur, Ozkan; Aslan, Mustafa Kemal; Ayva, Sebnem Kupana; Fedakar-Senyucel, Mine; Soyer, Tutku; Kisa, Ucler; Cakmak, MuratAim: This study aimed to evaluate the effect of P/E-selectin blockage on antisperm antibody (ASA) development and histopathological alterations in experimental orchitis. Materials and Methods: Thirty-six Wistar albino-type male rats weighing 100-150 g were included in the study. Rats were allocated into six groups (n = 6) including control (CG), sham (SG), orchitis (OG), antimicrobial treatment (AG), P/E-selectin blockage (PESG), and both antimicrobial and P/E-selectin treatment (TG) groups. In CG, serum samples were taken from the tail vein prior to the procedure and followed by extraction of both testes. In SG, 1 ml of saline solution was injected in testicular parenchyma. OG was obtained by injecting 0.1 ml 106 cfu/ml Escherichia coli (0: 6 strain) and 1 ml saline solution into the right testes. AG received ciprofloxacin (50 mg/kg/day) twice a day through gastrogavage 24 hours after generating orchitis. In PESG, P/E-selectin antibody (100 mu g) was administered intravenously via the tail vein 24 hours after the induction of orchitis. Finally, both ciprofloxacin and P/E-selectin antibody were administered in TG 24 hours after the induction of orchitis for 14 days. At the end of treatment, 1 ml of serum sample was obtained to evaluate the ASA, P-selectin and E-selectin levels. In order to evaluate spermatogenesis (Johnsen score) and testicular injury (Cosentino score), both testes were extracted at the end of the 14th day. Results: In orchitis-induced groups (OG, ATG, PSEG, TG), ASA levels were significantly increased at the 14th day when compared to SG (p < 0.05). In TG, ASA levels were decreased when compared to AG. However, similar alteration in ASA levels was not detected in PSEG (p > 0.05). In OG and AG, P-selectin levels were decreased at the 14th day when compared to levels observed on 0 day (p < 0.05). E-selectin levels on 0 day showed that each group had higher levels of E-selectin when compared to CG (p > 0.05). There was no significant difference regarding E-selectin when compared to CG (p > 0.05). No significant differences regarding E-selectin levels were detected on the 0th and 14th days between AG and CG (p > 0.05). When the Cosentino and Johnsen scores were compared among groups, TG and PSEG has decreased scores of Cosentino than OG on the right testicle (p < 0.05). In contrast, an increased Johnsen score was detected in TG and PSEG when compared to OG (p < 0/05). No significant difference was detected for both Cosentino and Johnsen scores on the left testicle (p > 0.05). There was no difference with regard to the right and left testicular injury in TG. In P/E-blocked groups, decreased histopathological alterations were observed in the contralateral testis. Conclusion: P/E-selectin blockage may reduce ASA production after orchitis when combined with antimicrobial treatment. P/E-selectin blockage not only has a protective effect on blood-testis barrier but also decreases the histopathological alterations in both the affected and contralateral testis. Histopathological parameters of spermatogenesis may also be prevented by P/E-selectin blockage in experimental orchitis. (C) 2013 Elsevier Inc. All rights reserved.Öğe Effect of phototherapy on growth factor levels in neonatal rat skin(W B Saunders Co-Elsevier Inc, 2011) Soyer, Tutku; Ayva, Sebnem; Aliefendioglu, Didem; Aktuna, Zuhal; Aslan, Mustafa Kemal; Senyucel, Mine Fedakar; Cakmak, MuratAim: Neonates undergoing surgery may receive phototherapy (PT) for the treatment of hyperbilirubinemia. Although the effects of PT on neonatal structures are well documented, the effect of PT on wound healing has not been previously evaluated. An experimental study was performed to evaluate the effect of PT on growth factor levels responsible for wound healing in neonatal rat skin. Materials and Methods: Eighteen Wistar newborn rats (7 +/- 2 g) were included in the study. Rats were randomized into 3 groups: control (CG), PT, and sham (SG) (n = 6). Both groups had 1-cm median dorsal skin incision. In CG, 1 x 1 cm of dorsal skin was sampled including the incised skin. The PT group received 5 banks of blue light (wave density, 30-40 mu w/cm(2) per nanometer; exposure distance, 45 cm). Phototherapy was started 24 hours after birth and exposed during light period (mean duration, 21 hours to 15 minutes perpendicular to 2 hour to 1.5 minutes). Sham group consisted of animals that received a bank of white light with same exposure distance and a total duration of 26 hours to 18 minutes +/- 3 hours to 9.1 minutes. After exposure, 1 x 1 cm dorsal skin samples were obtained from both PT and SG groups, including the median incision. The effect of PT was evaluated with the expressions of vascular endothelial growth factor (VEGF), its receptor (VEGF receptor), and transforming growth factor beta (TGF-beta) in endothelial vessels and fibroblasts of neonatal skin samples. Results: There was no significant difference between groups in VEGF receptor and transforming growth factor beta expressions. The VEGF levels in endothelial vessels were significantly decreased in PT and SG when compared with CG (P < .05). Conclusion: Vascular endothelial growth factor is a mediator of angiogenesis and may decrease in neonatal rat skin after light exposure. It can be suggested that decreased levels of VEGF after PT application may alter angiogenesis and also may adversely affect the healing features of neonatal skin. (C) 2011 Elsevier Inc. All rights reserved.Öğe The effect of platelet rich fibrin on growth factor levels in urethral repair(W B Saunders Co-Elsevier Inc, 2013) Soyer, Tutku; Ayva, Sebnem; Boybeyi, Ozlem; Aslan, Mustafa Kemal; Cakmak, MuratAim: Platelet rich fibrin (PRF) is an autologous source of growth factors and promotes wound healing. An experimental study was performed to evaluate the effect of PRF on growth factor levels in urethral repair. Materials and Methods: Eighteen Wistar albino rats were included in the study. Rats were allocated in three groups (n:6): control (CG), sham (SG), and PRF (PRFG). In SG, a 5 mm vertical incision was performed in the penile urethra and repaired with 10/0 Vicryl (R) under a microscope. In PRFG, during the urethral repair as described in SG, 1 cc of blood was sampled from each rat and centrifuged for 10 minutes at 2400 rpm. PRF obtained from the centrifugation was placed on the repair site during closure. Penile urethras were sampled 24 hours after PRF application in PRFG and after urethral repair in SG. Transforming growth factor beta receptor (TGF-beta-R-CD105), vascular endothelial growth factor (VEGF) and its receptor (VEGF-R), as well as endothelial growth factor receptor (EGFR), were evaluated in subepithelia of the penile skin and urethra. Groups were compared for growth factor levels and growth factor receptor expression with the Kruskal Wallis test. Results: TGF-beta-R levels were significantly decreased in SG when compared to CG (p < 0.05). In PRFG, TGF-beta-R was increased in both subepithelia of penile skin and urethra with respect to SG (p < 0.05). When VEGF levels and its receptor expression were compared between SG and PRFG, VEGF levels were found to be increased in penile skin subepithelium, whereas VEGF-R expressions were decreased in urethral subepithelia in PRFG (p < 0.05). There was no difference between groups for EGFR levels (p > 0.05). Conclusion: Use of PRF after urethral repair increases TGF-beta-R and VEGF expressions in urethral tissue. PRF can be considered as an alternative measure to improve the success of urethral repair. (C) 2013 Elsevier Inc. All rights reserved.Öğe The effects of ozone on the acute phase of intestinal ischemia-reperfusion injury in rats(2020) Günal, Yasemin Dere; Türer, Özlem Boybeyi; Atasoy, Pınar; Kısa, Üçler; Aslan, Mustafa KemalBACKGROUND: In this study, we aimed to examine the therapeutic effects of ozone on the acute phase of intestinal ischemia-reperfusion (I/R) injury in rats to resemble clinical practice. METHODS: Eighteen Wistar albino rats were assigned to control (CG, n=6), sham (SG, n=6) and ozone groups (OG, n=6). A midline laparotomy was performed and a superior mesenteric artery (SMA) in the SG and OG was occluded with a 0/0 catgut suture, but in the CG, the incision was closed without any intervention. Tissue oxygenation was monitored with a tissue oxygenation monitor to achieve the same grade during intestinal ischemia. The incision was closed and, in the OG, ozone/oxygen mixture (0.7 mg/kg) was injected intraperitoneally, 20 minutes before reperfusion. Surgical incision was reopened and reperfusion was achieved after 60 minutes of ischemia in the SG and OG. After 60 minutes of reperfusion, 2 cm small intestine segment was sampled for histopathological assessment of the intestinal mucosal damage (Chiu score) and biochemical assessment of oxidative stress markers (nitric oxide: NO, malondialdehyde: MDA, superoxide dismutase: SOD) in all groups. RESULTS: The Chiu scores of the SG and OG were statistically increased than that of the CG (p=0.002; and p=0.002, respectively). Chiu score in the OG was higher compared to that in the SG, but not statistically significant (p=0.175). MDA levels were statistically higher in the SG and OG than that of the CG (p=0.004; and p=0.010, respectively). However, the difference between the SG and OG was not statistically significant (p=0.522). SOD and NO levels were not significantly different between groups (p=0.451 and p=0.056, respectively). CONCLUSION: Contrary to the literature, single-dose ozone therapy did not reduce the oxidative stress or improve the ischemic damage in intestinal I/R injury in rats. Further evaluation with different doses in different time periods is needed for potential clinical use.Öğe Electrophysiologic evaluation of cremasteric reflex in experimental orchitis(Elsevier Sci Ltd, 2013) Soyer, Tutku; Inal, Elem; Boybeyi, Ozlem; Balci, Mahi; Aslan, Mustafa Kemal; Aydin, GulumserAim: Absent cremasteric reflex (CR) is a well known but not reliable sign of testicular torsion. We hypothesized that CR can also be altered in other causes of acute scrotum in children. An experimental study was performed to evaluate the clinical and electrophysiological features of CR in orchitis. Method: Eighteen Wistar albino rats were allocated into three groups: control (CG), sham (SG) and orchitis (OG). In CG, after anesthetization with ketamine hydrochloride, the medial site of the anterior superior iliac spine was stimulated to obtain CR electrophysiologically, and latency and duration were recorded with a needle electrode placed in the cremasteric muscle. Electrophysiologic evaluations were performed 24 h after injection of 0.1 ml of 10(6) cfu/ml Escherichia coli (0: 6 strain) in 1 ml of physiologic saline into the right testicle in OG, and 1 ml of saline only in SG. All testicles were sampled to check for orchitis after the electrophysiologic evaluations. Results: CR was obtained in all rats in CG and in 83.3% and 66.6% in SG and OG respectively (p < 0.05). The latency of CR was significantly higher in OG (15.1 +/- 0.9 ms) and SG (15.5 +/- 1.2 ms) than CG (10.5 +/- 0.7 ms) (p < 0.017). The duration of CR was 15.1 +/- 3.2 ms in CG, 16.2 +/- 4.9 ms in SG and 18.5 +/- 3 ms in OG (p > 0.05). Histopathologic confirmation of orchitis was obtained in all testicle samples in OG, and number of neutrophils and total orchitis score was significantly higher in OG than the other groups (p < 0.05). Conclusion: Electrophysiologic parameters of CR may be altered in orchitis. Prolonged latency of CR in orchitis may be due to inflammation of the genitofemoral nerve or cremasteric muscle. (C) 2013 Journal of Pediatric Urology Company. Published by Elsevier Ltd. All rights reserved.Öğe Evaluation of Dimethyl Sulfoxide and Dexamethasone on Pulmonary Contusion in Experimental Blunt Thoracic Trauma(Georg Thieme Verlag Kg, 2014) Boybeyi, Ozlem; Bakar, Bulent; Aslan, Mustafa Kemal; Atasoy, Pinar; Kisa, Ucler; Soyer, TutkuBackground A thoracic traumamodel was designed to evaluate the effect of dimethyl sulfoxide (DMSO) and dexamethasone (DX) on histopathologic and oxidative changes in lung parenchyma seen after pulmonary contusion. Methods Twenty-four Wistar albino rats were included in the study. They were allocated into control (CG, n = 6), sham (SG, n = 6), DX (DXG, n = 6), and DMSO (DMG, n = 6) groups. Only a lung biopsy was performed in CG. In the experimental groups, blunt thoracic trauma was induced by dropping a cylindrical metal weight (0.5 kg) through a stainless steel tube onto the right hemithorax from a height of 0.4 m (E = 1.96 J). In the SG, 1 mL of physiologic saline was injected intraperitoneally, in the DXG 10 mg/kg of DX was injected intraperitoneally, and in the DMG 1.2 g/mL of DMSO was injected intraperitoneally 15 minutes after trauma. After 6 hours, lung biopsy was performed for histopathologic and oxidative injury markers. Results Histopathologically, congestion, hemorrhage, neutrophil infiltration, endothelial-nitric oxide synthase (E-NoS), and total pathologic score were significantly higher in SG, DXG, and DMG when compared with CG (p < 0.05). Neutrophil infiltration, total pathologic score, and E-NoS were significantly decreased in DMG when compared with SG and DXG (p < 0.05). Biochemically, superoxide dismutase (SOD) level was significantly higher in SG, DXG, and DMG than in CG. SOD level was significantly lower in DXG and DMG than in SG (p < 0.05). Conclusion DMSO prevents further injury by decreasing neutrophil infiltration and endothelial injury in lung contusions. DX may have a role in the progression of inflammation but not in preventing the pathologic disruption of pulmonary parenchyma.Öğe Evaluation of intraabdominal hypertension and genitofemoral nerve motor conduction(2016) Türer, Özlem Boybeyi; İnal, Elem; Günal, Yasemin Dere; Aslan, Mustafa Kemal; Aydın, Gülümser; Soyer, TutkuBackground/aim: The aim of this study was to electrophysiologically evaluate the effect of increased intraabdominal pressure (IAP) on genitofemoral nerve (GFN) motor conduction. Materials and methods: Seven Wistar albino rats were included. After anesthetization, latency and duration of GFN conduction was recorded with a needle-probe at rest. IAP was increased to 15 mmHg by insufflating atmospheric air with a percutaneous intraperitoneal needle. At 30 min of IAP, GFN motor conduction was recorded. Abdominal pressure was then increased to 20 mmHg. At 60 min, GFN motor conduction was recorded again. The consecutive recordings of latency and duration of GFN conduction (rest, 30 min, 60 min) were evaluated statistically. Results: There was a significant difference between latencies at rest (1.90 ± 0.22 ms), at 30 min (2.3 ± 0.36 ms), and at 60 min (2.74 ± 0.57 ms) (Friedman test, P = 0.001). The latency was significantly increased at 60 min compared to rest (post hoc Tukey test, P = 0.003). No similar difference was detected between the recordings at 30 and 60 min. The duration of GFN motor conduction showed no difference between consecutive recordings (P = 0.067). Conclusion: Both increased and prolonged IAP causes prolonged latency of GFN conduction, probably due to a compression effect on GFN. Neuropraxial consequences of increased IAP are thought to be related to the compression effect of peripheral nerves.Öğe Evaluation of omental inflammatory response with P-/E-selectin levels and histopathologic findings in experimental model(W B Saunders Co-Elsevier Inc, 2012) Aslan, Mustafa Kemal; Boybeyi, Ozlem; Soyer, Tutku; Senyucel, Mine Fedakar; Ayva, Sebnem; Kisa, Ucler; Cakmak, MuratAim: The omentum plays a crucial role in abdominal defense mechanism by adhering to sites of inflammation and absorbing bacteria and debris from the peritoneal cavity. An experimental study was conducted to evaluate the inflammatory response of omentum in different abdominal events with omental P-/E-selectin levels and histopathologic findings. Materials and methods: Thirty Wistar rats were placed into 5 groups (n = 6), including a control group (CG), sham group (SG), bladder perforation (BP) group, splenic laceration (SL) group, and cecal ligation and puncture (CLP) group. Omental samples were obtained in CG after median laparotomy. In accordance with described models, BP, SL, and CLP were performed in experimental groups. Twenty-four hours after the first laparotomy, localization of the omental pad was noted, and omental samples were obtained for biochemical analysis of levels and histopathologic findings (no. of vessels in sections, polymorphic nuclear leukocytes [PMLs], lymphocytes). The mean P-/E-selectin levels and histopathologic findings of inflammation were compared between groups. Results: Although omentum was adhered to the cecum in all subjects after CLP, similar findings were not detected in other groups. P-selectin and E-selectin levels and number of PML were significantly increased in the CLP group when compared with other groups (P < .05). The number of vessels in sections was significantly increased in CLP group when compared with SG and BP groups (P < .05), and the BP group had a decreased number of vessels than CG (P < .05). The number of PML was significantly increased in SG and SL and BP groups with respect to CG (P < .05). Conclusion: Among different experimental intraabdominal catastrophes, only CLP caused an inflammatory response and increased levels of adhesion molecules in the omentum. These findings suggest that the nature of the inflammation is the main determining factor for the omental function in intraabdominal events. (c) 2012 Elsevier Inc. All rights reserved.Öğe Hirschsprung Hastalığında Transanal Endorektal Pull-through ve Martin-modifiye Duhamel Ameliyatlarının Sonuçlarının Karşılaştırılması(2019) Günal, Yasemin Dere; Aslan, Mustafa Kemal; Karaman, Ayşe; Karaman, İbrahim; Erdoğan, Derya; Çavuşoğlu, Yusuf HakanAmaç: Hirschsprung hastalığı (HH) tanısıyla transanal endorektal pull-through (TEPT) ve Martin-modifiye Duhamel ameliyatları yapılmış olan hastalarımızın sonuçlarının karşılaştırması. Gereç ve Yöntem: Kliniğimizde 2002-2007 yılları arasında HH nedeniyle TEPT (grup 1, n=24), transanal yolla başlanıp laparotomi gerektiren endorektal pull-through (grup 2, n=12) ve Martin modifiye Duhamel (grup 3, n=17) uygulanan 53 hasta değerlendirildi. Bu hasta grupları yaş, cinsiyet, aganglionik segment uzunluğu, intraoperatif detaylar, postoperatif erken dönem komplikasyonlar ve uzun dönem fonksiyonel sonuçlar açısından değerlendirildi ve karşılaştırıldı. Bulgular: Çalışmamızda grup 1 hastaların ortalama ameliyat yaşı, ameliyat süresi, oral beslenmeye başlama zamanı, ilk gaita çıkarma zamanı ve hastanede kalış süresi grup 2 ve grup 3 hastalarla karşılaştırıldığında anlamlı olarak az veya kısa bulundu (p<0,001). Ameliyat sırasında kan transfüzyonu ihtiyacı yüzde olarak grup 1 hastalarda daha az olmasına rağmen aradaki bu fark istatistiksel olarak anlamlı bulunmadı (p>0,05). Serimizde postoperatif enterokolit oranı grup 3’te yüzde olarak daha yüksek bulunmasına rağmen aralarındaki bu fark anlamlı bulunmadı (p>0,05). Her üç düzeltici tekniğin uzun dönem fonksiyonel sonuçları (kontinans, fekal soiling, kabızlık) değerlendirilip karşılaştırıldığında aralarında anlamlı bir fark bulunmadı (p>0,05). Sonuç: TEPT tekniği kolay uygulanabilen güvenli bir tekniktir. Özellikle erken dönemde tanı konmuş, enterokolit öyküsü olmayan, henüz bağırsak dilatasyonu gelişmemiş, rektosigmoid tutulumlu HH’lerde TEPT ilk tercih olabilir
