Comparison of the effect of contrast medium, air, and ozone on ischemia/reperfusion injury due to experimental intussusception model
Yükleniyor...
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tubitak Scientific & Technical Research Council Turkey
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Aim: To compare effects of contrast medium, air, and ozone on ischemia/reperfusion injury due to intussusception. Materials and methods: Thirty Wistar rats were allocated into five groups (n = 6). In the control group (CG), 2 cm of colonic segment, 5 cm distal to ileocecal valve, was removed. Intussusception was performed at 5 cm distal to the ileocecal valve by pushing a proximal intestinal segment distally by stile in experimental groups. One hour later, in the sham-operated group (SG), manual reduction was performed. In the reduction groups, reduction of intussusception was performed by contrast medium (CMG, 1/1 diluted omnipaqueR, infusion rate 2 mL/min), by air (AG, Pmax = 60 mmHg), or by ozone (OG, 10 mu g/mL, 0.5 mg/kg, Pmax = 60 mmHg). One hour after reduction, 2 cm of colonic segment was removed. Colonic tissues were evaluated for histopathological grades and oxidative injury markers (malondialdehyde [MDA], nitric oxide [NO], and total sulfhydryl [T-SH] values). Results: Mucosal injury score was significantly higher in AG then in CG or SG (P < 0.05). There was no difference regarding mucosal injury between the other groups (P > 0.05). MDA levels were significantly higher and NO levels were significantly lower in CMG, OG, and AG than in CG (P < 0.05). There was no difference between OG and the other experimental groups in both MDA and NO levels (P > 0.05). Conclusion: None of the reduction methods in intussusception have superiority over others in preventing ischemia/reperfusion injury.
Açıklama
KISA, Ucler/0000-0002-8131-6810; Soyer, Tutku/0000-0003-1505-6042
Anahtar Kelimeler
Intussusception, ozone, intestine, ischemia-reperfusion, injury, experimental
Kaynak
Turkish Journal Of Medical Sciences
WoS Q Değeri
Q3
Scopus Q Değeri
Q1
Cilt
43
Sayı
4
Künye
Fedakar M. Ş., Ayva E. Ş., Aslan M. K., Türer Ö. B., Soyer T., Aksoy N., Kısa Ü., Çakmak A. M. (2013). Comparison of the effect of contrast medium, air, and ozone on ischemia/reperfusion injury due to experimental intussusception model. Turkish Journal of Medical Sciences, 43(4), 637 - 641.
