Yazar "Aydinuraz, Kuzey" seçeneğine göre listele
Listeleniyor 1 - 11 / 11
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Effect of hypothermia on apoptosis in traumatic brain injury and hemorrhagic shock model(Elsevier Sci Ltd, 2017) Eroglua, Oguz; Deniz, Turgut; Kisa, Ucler; Atasoy, Pinar; Aydinuraz, KuzeyIntroduction: The neuroprotective mechanisms of therapeutic hypothermia against trauma-related injury have not been fully understood yet. In this study, we aimed to investigate the effects of therapeutic hypothermia on biochemical and histopathological markers of apoptosis using Traumatic brain injury (TBI) and hemorrhagic shock (HS) model. Methods: A total of 50 male albino-wistar rats were divided into five groups: Group isolated TBI, Group NT (HT + HS + normothermia), Group MH (HT + HS + mild hypothermia), Group MoH (HT + HS + moderate hypothermia) and Group C (control). Neurological deficit scores were assessed at baseline and at 24 h. The rats were, then, sacrificed to collect serum and brain tissue samples. Levels of Caspase-3,6,8, proteoglycan-4 (PG-4), malondialdehyde (MDA), and nitric oxide (NO) were measured in serum and brain tissue samples. Histopathological examination was performed in brain tissue. Results: There were significant differences in the serum levels of Caspase-3 between Group NT and Group C (p = 0.018). The serum levels of Caspase-6 in Group NT (0.70 +/- 0.58) were lower than Group MH (1.39 +/- 0.28), although the difference was not statistically significant (p = 0.068). There were significant differences in the brain tissue samples for Caspase-3 levels between Group NT and Group C (p = 0.049). A significant difference in the Caspase-8 brain tissue levels was also observed between Group NT and Group C (p = 0.022). Group NT had significantly higher scores of all the pathological variables (for edema p < 0.017; for gliosis p < 0.001; for congestion p < 0.003, for hemorrhage p < 0.011) than Group C. Conclusion: Our study results suggest that hypothermia may exert its neuroprotective effects by reducing markers of apoptotic pathway, particularly Caspase-3 on TBI and HS. (c) 2017 Elsevier Ltd. All rights reserved.Öğe The effect of thymoquinone coating on adhesive properties of polypropylene mesh(Bmc, 2017) Aydin, Oktay; Aydinuraz, Kuzey; Agalar, Fatih; Sahiner, I. Tayfun; Agalar, Canan; Bayram, Cem; Atasoy, PinarBackground: An incisional hernia is a common complication following abdominal surgery. Polypropylene mesh is frequently used in the repair of such defects and has nearly become the standard surgical treatment modality. Though they are very effective in reducing recurrence, mesh materials exhibit a strong stimulating effect for intraabdominal adhesion. The thymoquinone (TQ) extracted from Nigella sativa seeds has potential medical properties. TQ has antiinflammatory, antioxidant and antibacterial properties. The aim of this study is to coat polypropylene mesh with TQ in order to investigate the effect of surface modification on intraabdominal adhesions. Methods: TQ-coated polypropylene mesh material was tested for cytotoxicity, contact angle, surface spectroscopy, TQ content, sterility, and electron microscopic surface properties. An experimental incisional hernia model was created in study groups, each consisting of 12 female Wistar rats. The defect was closed with uncoated mesh in control group, with polylactic acid (PLA)coated mesh and PLA-TQ coated mesh in study groups. Adhesion scores and histopathologic properties were evaluated after sacrifice on postoperative 21th day. Results: Granuloma formation, lymphocyte and polymorphonuclear leukocyte infiltration, histiocyte fibroblast and giant cell formation, capillary infiltration, collagen content were significantly reduced in the PLA-TQ coated mesh group (p < 0.05). Though not statistically significant, likely due to the limited number of study animals, adhesion formation was also reduced in the PLA-TQ coated mesh group (p: 0.067). Conclusion: TQ coated mesh is shown to reduce adhesion formation and TQ is a promising coating material for mesh surface modification.Öğe Effects of basic fibroblast growth factor and phenytoin on healing of abdominal wall fascia and colonic anastomoses(Int College Of Surgeons, 2006) Güneş, Hayrettin Varol; Demirer, Seher; Aydinuraz, Kuzey; Kepenekci, Ilknur; Kuterdem, Ercüment; Aribal, DikmenThe aim of this study was to investigate the effects of basic fibroblast growth factor (bFGF) and phenytoin on wound healing in rats compromised by methylprednisone. This study was conducted in four groups consisting of 20 male Wistar rats. Rats in groups 2, 3, and 4 had a daily injection of methylprednisolone 5 mg/kg/day for 15 days. Laparotomy and sigmoid transsection were performed on day 15. In the postoperative period, rats in group 1 received no medication, group 2 received methylprednisolone 5 mg/kg/day intramuscularly, group 3 received bFGF 5 mu g/kg on days 1-3 subcutaneously, and group 4 received phenytoin 40 mg/kg/day intraperitoneally. bFGF and phenytoin had a positive effect on tensile strength, hydroxyproline content, and wound healing parameters in abdominal wall fascia. In colonic anastomosis, phenytoin corrected all parameters, but bFGF had no effect.Öğe Effects of different anesthetic techniques on serum leptin, C-reactive protein, and cortisol concentrations in anorectal surgery(Medicinska Naklada, 2006) Büyükkoçak, Ünase; Daphan, Çağatay; Çağlayan, Osman; Aydinuraz, Kuzey; Kaya, Tahsin; Saygun, Oral; Ağalar, FatihAim To compare the effects of intratracheal general anesthesia (ITGA) and regional (saddle block) anesthesia on leptin, C-reactive protein (CRP), and cortisol blood concentrations during anorectal surgery. Methods Fifty-eight patients suffering from hemorrhoidal disease, pilonidal sinus, anal fissure, or anal fistula were included the study. Patients were randomly assigned into one of the two groups (n = 29). Patients in one group received ITGA. After thiopental and fentanyl induction, vecuronium was used as a muscle relaxant. Anesthesia was maintained with sevoflurane. In the other group we applied saddle block, injecting hyperbaric bupivacaine into the subarachnoid space, through the L3-L4 intervertebral space, in the sitting position. Blood samples were collected for leptin, CRP, and cortisol analysis before the induction of anesthesia at 3 and 24 hours postoperatively. Results Preoperative leptin, CRP, and cortisol concentrations were comparable between the groups. There was no significant difference in postoperative levels of leptin and CRP in both groups. Although not significant, leptin and CRP concentrations were lower in the saddle block group at three hours postoperatively (mean SD, 6.95 +/- 8.59 and 6.02 +/- 12.25, respectively) than in the ITGA group (mean SD, 9.04 +/- 9.89 and 8.40 +/- 15.75, respectively). During early postoperative period, cortisol increased slightly in the ITGA group and remained at similar level in the saddle block group, but later decreased in both groups. Cortisol levels in the saddle block group were significantly lower than in the ITGA group at 3 hours postoperatively (343.7 +/- 329.6 vs 611.4 +/- 569.8; P = 0.034). Conclusion Saddle block, a regional anesthetic technique, may attenuate stress response in patients undergoing anorectal surgery, by blocking afferent neural input during early postoperative period.Öğe The Effects of Simvastatin on Ischemia Reperfusion Injury in an Experimental Colon Anastomosis Model(Springer India, 2017) Akarsu, Mahmut; Saygun, Oral; Aydinuraz, Kuzey; Aydin, Oktay; Daphan, Cagatay Erden; Tanrikulu, Fatma Benli; Comu, Faruk MetinAnastomotic leakage is more frequently reported in colonic anastomoses. Ischemia reperfusion injury is one of the main reasons for anastomotic leakage. Simvastatin is known to prevent tissue damage induced by free oxygen radicals after ischemia reperfusion injury. The effect of simvastatin on colonic anastomosis impaired by ischemia reperfusion injury is investigated. Single layer, end-to-end colocolic anastomosis after 0.5-cm colon resection was performed in Wistar Albino rats. In Group 1 (control) (n= 10), colonic anastomosis without I-R was performed. In Group 2 (n = 10), the superior mesenteric artery was clamped for 10 min followed by 60 min of reperfusion after which resection anastomosis was performed. In Group 3 (n= 10), 10 mg/kg simvastatin was given by gavage for 7 days after I-R and resection anastomosis. In Group 4 (n = 10), the rats received 10 mg/kg simvastatin by gavage 7 days before and 7 days after ischemia reperfusion and surgery. All of the rats were sacrificed 8 days after surgery. Anastomotic bursting pressure and tissue hydroxyproline levels were measured. Postoperative administration of simvastatin restored the anastomotic bursting pressure and hydroxyproline levels to that of control group thus overcoming the effect of ischemia reperfusion injury. Simvastatin administered postoperatively in an experimental model of colonic resection anastomosis impaired by ischemia reperfusion injury increased anastomotic bursting pressures and tissue hydroxyproline levels. Further experimental and clinical studies will show whether administration of simvastatin will increase reliability of the anastomosis and decrease postoperative morbidity and mortality in colonic anastomosis after ischemia reperfusion injury.Öğe The Effects of Thymoquinone (Kalonji) on Abdominal Adhesion in Experimental Abdominal Adhesive Model(SPRINGER INDIA, 2020) Ozden, Huseyin; Saygun, Oral; Daphan, Cagatay E.; Aydinuraz, Kuzey; Aydin, Oktay; Tanrikulu, Fatma Benli; Dom, SedatThe aim of this study was to investigate the effects of thymoquinone on abdominal adhesion development in an experimental abdominal adhesion model. Forty-five female Wistar albino rats weighing 260-280 g were used in the study. The rats were randomized into 3 even groups. Control, serum physiological (SP), and thymoquinone (TQ) groups were formed. In the experimental abdominal adhesion model, caecum serosa was brushed until the petechial hemorrhages were seen. No additional procedures were performed in the control group except surgery protocol. SP was injected over the caecum in the SP group. In the thymoquinone group, the abdomen was closed after the application of prepared thymoquinone solution on the caecum surface. On the 21st day, tissue samples from sacrificed rats were examined macroscopically and microscopically, and statistically evaluated. There was a statistically significant difference between the control group and the thymoquinone group in the microscopic evaluation (p = 0.006). However, there was no statistically significant difference between SP group and control group, and between thymoquinone group and SP group. Macroscopic evaluation revealed a statistically significant difference between the thymoquinone group and the control group, and between the thymoquinone group and the SP group (p = 0.009,p = 0.027, respectively). In the microscopic and macroscopic evaluation, it was observed that thymoquinone had a decreasing effect on postoperative adhesions. We think that this effect of thymoquinone is due to its anti-inflammatory and antioxidant properties. However, the available data are not sufficient for this effect to be used in medical treatment. Further studies are needed in the future.Öğe The evaluation of academic emergency department design(Turkish Assoc Trauma Emergency Surgery, 2007) Deniz, Turgut; Aydinuraz, Kuzey; Oktay, Cem; Saygun, Meral; Agalar, FatihBACKGROUND In our study which was based upon a questionnaire, the inner and outer architectural designs of emergency services of Emergency Medicine Departments were investigated. METHODS In this descriptive study, a standard questionnaire was sent to 26 Emergency Medicine Departments which were operating at that time. In the questionnnaire, the internal, external architectural and functional features were questioned. Answers of 22 Emergency Medicine Departments were analysed. Two Emergency Medicine Departments that were not operating at that time were not included in the study. RESULTS The analysis of the replies revealed that only 59% (n=13) of the Emergency Medicine Departments were designed as an emergency service prior to the construction. The ambulance parking areas were not suitable in 77% of the emergency units while only 54.5% (n=12) had protection against adverse weather conditions. In only 59% (n=13) of the emergency units, a triage unit was present and in only one of the in only one (4.5%), a decontamination room was available. It was understood that only 32% (n=8) of the emergency units were appropriate in enlarging their capacity taking the local risk factors into consideration. There was a toilette for disabled patients in only 18% (n=4) of the units as well. Considering a 12-year of history of the Emergency Medicine in Turkey, the presence of a lecture room is still 68% (n=15) in emergency departments which reflects that academical efforts in this field is emerging in challenging physical conditions. CONCLUSION The results of our study revelaed that emergency service architecture was neglected in Turkey and medical care given was precluded by the insufficient architecture. The design of emergency services has to be accomplished under guidance of scientific data and rules taking advices of architects who have knowledge and experience on this field.Öğe In Vitro S. epidermidis and S. aureus Adherence to Composite and Lightweight Polypropylene Grafts(Academic Press Inc Elsevier Science, 2009) Aydinuraz, Kuzey; Agalar, Canan; Agalar, Fatih; Ceken, Sabahat; Duruyurek, Nihal; Vural, TayfunBackground. Despite the significant risk of infection, prosthetic graft materials have become indispensable for incisional hernia repair. Composite and lightweight grafts have been developed to overcome adhesion and rigidity. The aim of this study was to evaluate in vitro S. epidermidis and S. aureus adherence to these new generation grafts, which have modified composition and textile properties. Materials and Methods. In this experimental study, sterile samples of multifilament polypropylene, multifilament lightweight composite, monofilament composite, and monofilament polypropylene grafts were incubated with slime positive S. epidermidis and S. aureus strains for 24 h. The grafts were washed and vortexed in saline. The saline was passaged to blood agar and incubated for 24 h. The colonies were counted. Naive and incubated graft materials were examined by scanning electron microscopy to reveal both textile properties and biofilm formation. Physicochemical properties were evaluated. Wilcoxon signed ranks test and Friedman test were used for statistical analysis. Results. S. epidermidis showed a significantly decreased adherence to monofilament polypropylene graft, and increased adherence to multifilament polypropylene graft (P<0.05). S. aureus showed a significant affinity for monofilament composite graft (P<0.05). SEM studies showed that biofilm formation was present on multifilament polypropylene graft. Conclusions. Both S. epidermidis and S. aureus had significantly less adherence to multifilament lightweight composite than multifilament polypropylene graft. S. epidermidis showed a greater affinity to monofilament composite graft compared with monofilament polypropylene graft. Overall S. epidermidis adherence for multifilament lightweight composite and monofilament composite was less than multifilament polypropylene and higher than monofilament polypropylene. S. epidermidis and S. aureus showed different adherence for each graft. Changes in material composition and textile properties may well influence bacterial adherence. (C) 2009 Elsevier Inc. All rights reserved.Öğe Significance of Intra-abdominal Free Fluid Detected in Ultrasonography in the Clinical Assessment and Outcomes of Adult Patients Presenting to the Emergency Department Due to Abdominal Pain(Cureus Inc, 2019) Erkek, Aylin; Aydin, Yasemin Yilmaz; Ciftci, Handan; Ramadan, Hayri; Temiz, Kerim; Aydinuraz, Kuzey; Coskun, FigenObjective The aim of the study was to evaluate the diagnostic process and clinical course in adult patients who presented to the emergency department (ED) with acute abdominal pain (AAP) and were found to have intra-abdominal free fluid (FF) on ultrasonography (USG). Methods This prospective observational study was conducted in a training and research hospital adult emergency department between March 15, 2013, and April 15, 2013. The study included 252 patients aged above 18 years, who were admitted to the emergency room complaining of nontraumatic acute abdominal pain and provided consent for the study. Results The most common diagnoses were acute, nonspecific abdominal pain (37.3%), acute appendicitis (19%), and urinary tract pathology (15.9%). Intra-abdominal free fluid was detected with ultrasonography in 42.5% of patients. Patients with intra-abdominal free fluid were younger than the other patients. The emergency department length of stay was longer in patients with intra-abdominal free fluid (p=0.011). Of the 252 patients enrolled in the study, 32.9% were admitted to the hospital, 21.4% of whom underwent surgery and 11.5% received medical therapy. Most of the patients (64.5%) who were discharged home had no intraabdominal free fluid in the ultrasonography (p<0.001). Conclusion The presence of intra-abdominal free fluid alone did not guide the clinical decision regarding the diagnostic evaluation of adult patients that presented to the emergency department complaining of non-traumatic acute abdominal pain.Öğe Similar effects of general and spinal anaesthesia on perioperative stress response in patients undergoing haemorrhoidectomy(Hindawi Ltd, 2006) Büyükkoçak, Ünase; Cağlayan, Osman; Daphan, Cağatay; Aydinuraz, Kuzey; Saygun, Oral; Kaya, Tahsin; Agalar, FatihSurgery induces release of neuroendocrine hormones (cortisol), cytokines (interleukin-6: IL-6, tumour necrosis factor-alpha: TNF-alpha), acute phase proteins (C-reactive protein: CRP, leptin). We studied the effects of general and spinal anaesthesia on stress response to haemorrhoidectomy. Patients were assigned to general and spinal anaesthesia groups (n = 7). Blood samples were drawn before induction and 24 hours after surgery. Perioperative levels of IL-6, TNF-alpha, CRP, cortisol, and leptin were comparable among the groups. Twenty four hours after surgery, TNF-alpha and cortisol did not change; IL-6 and CRP increased significantly in all patients. Significant increase in leptin levels was found in patients undergoing spinal anaesthesia. Except for the increase in leptin levels, there was no significant difference related to the effects of general and spinal anaesthesia. Copyright (c) 2006 Unase Buyukkocak et al.Öğe The value of CRP, IL-6, leptin, cortisol, and peritoneal caspase-3 monitoring in the operative strategy of secondary peritonitis(Turkish Assoc Trauma Emergency Surgery, 2011) Pehlivanli, Faruk; Agalar, Fatih; Agalar, Canan; Saygun, Oral; Daphan, Cagatay; Aydinuraz, Kuzey; Sahiner, TayfunBACKGROUND We aimed to investigate the impact of C-reactive protein (CRP), interleukin (IL)-6, leptin, cortisol, and caspase-3 on the decision of terminating planned abdominal repair in secondary peritonitis. METHODS Fifteen patients with peritonitis were enrolled into the study. Serum CRP, IL-6, leptin, cortisol, and peritoneal caspase-3 activities were measured. RESULTS APACHE II scores at 48 hours (h) and age were significantly higher in non-survivors. A significant decrease was observed in caspase-3 activities of patients in whom <= 4 laparotomies were performed when compared with those who underwent >4 laparotomies. For patients who underwent <= 4 laparotomies, there was a significant difference in caspase-3 levels between 0 and 72 h. There was no significant difference in caspase-3 levels in non-survivors; caspase-3 levels were significantly lower in the survivors at 48 and 72 h. Changes in CRP, IL-6, leptin, and cortisol levels were not statistically significant. CONCLUSION CRP, IL-6, leptin, cortisol, and caspase-3 are not valuable in discriminating the number of planned operations, even though there is a significant decrease in caspase-3 "within" survivors. The discriminative value of caspase-3 for closure should be evaluated in studies in which caspase-3 is monitored for a longer duration in a large number of patients.
