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Öğe An Investigation of Heavy Metal Concentrations in the Sera of Cattle Grazed in Different Locations in the Kars Province of Türkiye(Springer, 2024) Tarhane, Ayse Kanici; Aluc, Yasar; Kiziltepe, Semistan; Ekici, HusamettinThe aim of this study was to investigate the effects of exposure of cattle to low levels of environmental pollution on trace metal metabolism. The assessment of heavy metal concentrations in blood samples is essential to evaluate the potential effects of pollutants on cattle production and to measure the uptake of pollutants by animals. For this purpose, cattle raised in villages at varying distances to the center of the Kars province were sampled for blood, and heavy metal concentrations were measured in the extracted sera. In total 150 blood samples were collected from 4-to 5-year-old cattle from 15 regions. Sera were extracted from the blood samples from the selected foci and analyzed with an inductively coupled plasma-optical emission spectrometry (ICP-OES) device for essential (Co, Cr, Cu, Fe, Mn, Ni and Zn, Se, V) and non-essential (Pb, Cd and Hg, As, Al, Sn) heavy metals. Heavy metals were detected in the serum samples in the following order: Sn > Fe > Al > Zn > Cu > Pb > Cr > As > V > Ni > Mn > Hg. A confidence interval of 95% was used to evaluate all tests. Differences between the villages were insignificant for the Cu, Pb, Cd, Hg, Se, Al, Co concentrations (p > 0.05), whereas differences between the villages were significant for the Fe, As, Ni, Mn (p < 0.05), Zn, Cr (p < 0.01), V and Sn (p < 0.001) concentrations. The results of this study show that heavy metals detected in bovine sera from the sampling area do not exceed the maximum permissible limits, suggesting limited exposure to heavy metals and no associated health risk to animals in the region.Öğe Comparing levels of certain heavy metals and minerals and antioxidative metabolism in cows raised near and away from highways(Scientific Technical Research Council Turkey-Tubitak, 2015) Ekici, Husamettin; Simsek, Ozkan; Arikan, Sevket; Eren, Meryem; Guner, BayramThe scope of this study was to investigate the concentrations of certain heavy metals, mineral levels, and antioxidative metabolism in cows raised near (300 m) and away from highways (2.5 km) in Cankiri Province. For this purpose, blood samples were collected from 100 cows (aged 3-5 years) raised near and away from highways in this province. Concentrations of certain heavy metals and minerals in the serum were analyzed on an ICP-MS device. Additionally, lipid peroxidation product malondialdehyde (MDA) in the plasma and antioxidative defense system indicators, erythrocyte superoxide dismutase (SOD) and catalase (CAT) activities, were measured with a spectrophotometer. As a result, levels of certain heavy metals and minerals and antioxidative status were determined in cows raised near and away from highways. Aluminum, iron, nickel, and zinc levels in the cows raised near highways were significantly different from those of the cows raised away from highways. Furthermore, MDA concentrations and SOD activities were significantly different between the two groups. It is concluded that monitoring the concentration of these heavy metals periodically may be useful to increase the productivity of cows.Öğe Comparison of Flow Cytometric Analysis and Eosin-Nigrosin Staining Methods for Determining some Morphological Characteristics of Bull Epididymal Spermatozoa(Kafkas Univ, Veteriner Fakultesi Dergisi, 2015) Akyol, Numan; Azkur, Ahmet Kursat; Ekici, HusamettinThe aim of this study is to investigate necrosis and apoptosis in epididymal bull spermatozoa before freezing and after thawing using the flow cytometric method and to compare this with eosin-nigrosin dyeing, which is the conventional method used in assessing of spermatozoa. The testicles from fourteen bulls at local slaughterhouse were used for this study. The proportions of live spermatozoa, total apoptotic, necrotic and early necrotic spermatozoa levels were observed via flow cytometry. Annexin V/ PI fluoruscence dyeing was used to investigate the proprotions of apoptotic, necrotic, early necrotic and live spermatozoa for flow cytometry. The proportion of dead spermatozoa and protoplasmic droplets were determined using the eosin-nigrosin conventional dyeing method in fresh and frozen-thawed spermatozoa. The average dead spermatozoa count with flow cytometry was less than with the eosin-nigrosin method (P < 0.05). Some morphological characteristics such as protoplasmic droplets could be determined with the eosin-nigrosin method; however, sperm subpopulations entering the death process (apoptotic, necrotic and early necrotic) could be defined clearly only with the flow cytometric method. As a result, combination of eosin-nigrosin dyeing method and flow cytometric analysis of sperm morphological evaluation could give better results of bull epididymal semen in comparison to eosin-nigrosin dyeing method alone.Öğe Comparison of the Algan Hemostatic Agent with Floseal in Rat Liver Laceration Bleeding Model(Aves, 2022) Binnetoglu, Kenan; Kumandas, Ali; Ekici, Husamettin; Ozbaykus, Abdullah CanberkObjective: Major vascular injury is one of the most important causes of death after trauma. 'The effective and speedy control of the hemorrhage is crucial in reducing deaths. Many products are used for this purpose. Today, however, an ideal product has not yet been produced and there is a strong demand for such effective hemostatic products. The aim of this study is to compare the efficacy of Algan hemostatic agent with Floseal in the liver laceration model in rats. Materials and Methods: A total of 28 rats were used in the study. Rats were divided into 4 groups, each consisting of 7 rats. Experimental liver laceration was established. In the control group. saline-impregnated gauze was applied. Algan hemostatic agent-impregnated sponge, Algan hemostatic agent powder, and Floseal gel were applied to the experimental groups. Results: There was no difference in bleeding control among the Algan hemostatic agent powder. Algan hemostatic agent-impregnated sponge, and Floseal. When compared to the control group, Algan hemostatic agent powder, Algan hemostatic agent-impregnated sponge, and Floseal were found to be very effective in bleeding control, respectively (P = .001, .012, and .002), in the experimental groups. Conclusion: This study showed that the properties of both Algan hemostatic agent powder and Algan hemostatic agent-impregnated sponge for controlling bleeding are similar to Floseal. Considering other characteristics such as Algan hemostatic agent's naturalness, easy applicability, and low cost, Algan hemostatic agent has been a promising effective hemostatic agent.Öğe Concentration of essential and non-essential elements and carcinogenic/non-carcinogenic health risk assessment of commercial bee pollens from Turkey(Elsevier Gmbh, 2023) Sevin, Sedat; Tutun, Hidayet; Yipel, Mustafa; Aluc, Yasar; Ekici, HusamettinBackground: Bee pollen, known as a natural super-food with valuable nutritional ingredients, is regarded as a good indicator of ecotoxic substances, such as potentially toxic elements (PTEs). Therefore, this study aims to examine the concentrations of selected PTEs (Al, As, Ba, Cd, Co, Cr, Cu, Fe, Hg, Mg, Mn, Mo, Ni, Pb, Se, Sn, Sr, V, Zn) in bee pollen purchased from online markets in Turkey and perform a health risk assessment to identify the potential risk to consumers.Methods: The quantitative analyses were conducted by inductively coupled plasma optical emission spectrometry (ICP-OES).Results: The mean values of essential PTEs in decreasing content order were Mg > Fe > Zn > Mn > Cu > Ni > Se > Cr > Mo >Co = V. Regarding the results of the study, daily consumption (40 g for adult or 20 g for children) of commercial bee pollen can recompense 20-35 % of daily Cu, Mn, Se requirements for children, adults, pregnant, and breastfeeding women. The decreasing content order of non-essential elements was Al > Sn > Sr > Ba > Pb > As. Cadmium and Hg concentrations were below the detection limits in all the samples. In terms of food and public health; detection of the PTEs concentrations is necessary to assess the quality and safety of bee pollen before consumption. According to the carcinogenic and non-carcinogenic risk assessments; commercial pollen consumption does not pose a health risk to either children or adults for the PTEs monitored in this study.Conclusion: We conclude that bee pollen is an ideal indicator for the monitoring of environmental pollution of PTEs and also a valuable source of essential elements. This study highlights the need to develop standards that regulate acceptable concentrations of PTEs.Öğe Determination of the Metal Contents of Honey Samples from Orumieh in Iran(Kafkas Univ, Veteriner Fakultesi Dergisi, 2012) Saghaei, Shahram; Ekici, Husamettin; Demirbas, Mevlut; Yarsan, Ender; Tumer, IlyasIn this study the concentrations of metals in 89 different honey samples were collected, from four different areas of Orumieh City, in Iran from September of 2009 were investigated. Most of the samples were obtained from beekeepers located at a distance of 10 km from the roadside, to their accommodation. Pb, Co, Cr, Fe, Mn, Zn, As and Ni were detected in all of the honey samples. The contents of metals in honey samples were found to be in the range of 0.04 +/- 0.1 ppm, 0.001 +/- 0.002 ppm, 7.09 +/- 9.4 ppm, 0.6 +/- 0.9 ppm 0.06 +/- 0.1 ppm, 9.99 +/- 26.5 ppm, 0.0008 +/- 0.0011 ppm, 0.003 +/- 0.005 ppm for Pb, Co, Cr, Fe, Mn, Zn, As and Ni respectively. At the end of the study the Pb levels were found to be lower than the maximum residue limits of the European Union. Other metal levels were within acceptable levels. It was detected that the honey which was collected was of good quality from the point of metal contents.Öğe The Effect of Chlorpyrifos on Isolated Thoracic Aorta in Rats(Hindawi Ltd, 2013) Yildirim, Ebru; Baydan, Emine; Kanbur, Murat; Kul, Oguz; Cinar, Miyase; Ekici, Husamettin; Atmaca, NurgulThis study investigated the effect of chlorpyrifos on thoracic aorta and on the level of NO in plasma and aorta. The effect of chlorpyrifos on thoracic aorta in organ bath was determined in 10 rats. Another 45 rats were assigned to 3 groups with 15 rats each: control group 1 received distilled water, control group 2 was given corn oil, and the last group was given 13.5 mg/kg chlorpyrifos dissolved in corn oil every other day for 8 weeks orally. Chlorpyrifos (10(-10) M-10(-5) M) showed no effect on isolated thoracic aorta. Plasma AChE activity was decreased, while LDH, ALT, GGT, and AST activities were increased in chlorpyrifos group compared to control groups. Plasma NO level was increased in chlorpyrifos group compared to control groups. iNOS expression was present in all groups in the cytoplasm of the endothelia and in the smooth muscle cells of aorta. According to semiquantitative histomorphological analysis, iNOS immunopositive reactions were seen in the decreasing order in chlorpyrifos, control 2, and control 1 groups. eNOS immunopositive reactions were observed in the endothelial cell cytoplasm, rarely in the subintimal layer, and the smooth muscle cells of aorta. There were no differences among the groups in terms of eNOS immunostaining. In conclusion, chlorpyrifos induced NO production in aorta following an increase in NOS expression.Öğe Effect of Cocoa Butter and Sunflower Oil Supplementation on Performance, Immunoglobulin, and Antioxidant Vitamin Status of Rats(Hindawi Publishing Corporation, 2014) Yildirim, Ebru; Cinar, Miyase; Yalcinkaya, Ilkay; Ekici, Husamettin; Atmaca, Nurgul; Guncum, EnesThis study investigated the effects of cocoa butter and sunflower oil alone and in combination on performance, some biochemical parameters, immunoglobulin, and antioxidant vitamin status in Wistar rats. Forty-eight male rats were assigned to four groups, consisting of 12 rats with 3 replicates. Control received balanced rat diet without oil, cocoa butter group received 3.5% cocoa butter, sunflower oil group received 3.5% sunflower oil, the last group received 1.75% sunflower oil + 1.75% cocoa butter supplementation in the rat diet for 8 weeks. The total feed consumption in sunflower oil group was statistically lower than in the other groups. The serum creatinine level was decreased in cocoa butter group compared to control. Triglyceride and VLDL cholesterol levels were decreased in only sunflower oil and only cocoa butter groups as compared to control. The level of Ig M was statistically lower in cocoa butter and cocoa butter + sunflower oil groups than in control and sunflower oil groups. There were no statistically important difference in vitamin concentrations among trial groups. It was concluded that the supplementation of cocoa butter in diet decreased Ig M level, while the supplementation of cocoa butter and sunflower oil alone decreased the triglyceride and VLDL cholesterol levels.Öğe Effect of Different Conservation Conditions on the Active Compounds and Pharmacovigilance Screening of Different Florfenicol Preparations(Kafkas Univ, Veteriner Fakultesi Dergisi, 2013) Ekici, Husamettin; Yarsan, EnderThis study was aimed to evaluate the state of utilization of florfenicol, along with the investigation of adverse drug reactions and to evaluate the active substance content in different storage conditions. In this study, three different preparations were kept in different storage conditions and active substance content along with physical composition was determined at the beginning, 3rd, 6th and 9th months. No physical difference was observed for different condition storage preparations. In pharmacovigilance surveys; adverse drug declarations in which veterinarian reported were mostly from the second drug, following the third drug and the first drug lastly. Overall, active substance content lost was seen for the first drug groups. No significant difference of florfenicol levels (P>0.05) was observed for the second and the third drug groups stored both in room temperature and +4 degrees C for the initial, 3rd, 6th and 9th month periods.Öğe The effect of seasonal variations on the occurrence of certain mycotoxins in concentrate feeds for cattle collected from some provinces in Turkey(Scientific Technical Research Council Turkey-Tubitak, 2016) Ekici, Husamettin; Yildirim, Ebru; Yarsan, EnderThe aim of this study was to determine the effects of seasonal variations on the occurrence of total aflatoxin (AFTotal), aflatoxin B1 (AFB1), ochratoxin A (OTA), and total fumonisin (FTotal) in the commercially mixed ruminant feed obtained from feed mills located in Ankara, Kirikkale, Cankiri, Corum, and Kirsehir provinces using the enzyme-linked immunoassay (ELISA) method. To represent the annual production, 22 samples were collected every quarter over 1 year (a total of 88 samples) starting in September 2012. AFTotal and AFB1 were detected in 72 samples (81.81%), OTA in 84 samples (95.45%), and FTotal in 83 samples (94.31%). When seasonal changes were taken into consideration, the highest levels of AFTotal, AFB1, and FTotal (13.57 +/- 8.78 ppb, 8.54 +/- 6.02 ppb, and 0.70 +/- 0.88 ppm, respectively) were found in the summer, while the highest OTA level (57.69 +/- 14.59 ppb) was observed in the spring. The results of high-performance liquid chromatography methods confirmed the results of the ELISA method. Consequently, although most of the feed samples were contaminated with mycotoxins, all the amounts were within the limits allowed for feedstuffs in Turkey.Öğe Effectiveness of Algan Hemostatic Agent in Bleeding Control: An Experimental Kidney Incision Model(Galenos Yayincilik, 2021) Midi, Ahmet; Kumandas, Ali; Ekici, Husamettin; Kilic, Yusuf; Ozgocmen, RuchanAim: The purpose of this study is to evaluate the hemostatic efficacy of a novel plant originated blood stopper which is called Algan Hemostatic Agent (AHA) in uncontrolled kidney incision bleeding model. Methods: The study was conducted with thirty-two rats 5-7 weeks old. The rats were randomly divided into 4 groups, each consisting of eight rats (Group 1: heparinized saline, 2: heparinized AHA, 3: non-heparinized saline, 4: non-heparinized AHA). Experimental kidney incision was made, and saline soaked sponge was applied to the control group and AHA to the study group. Results: The duration of bleeding was significantly shorter in the AHA group compared to the control group. The AHA has controlled the bleeding in the heparinized and non-heparinized groups 5 +/- 1.19 and 3 +/- 0.91 seconds respectively (p<0.001). In the control group, hemorrhage was controlled in the heparinized and non-heparinized group 180.6 +/- 25.4 and 125.4 +/- 21 seconds respectively. Conclusion: This study demonstrated that the AHA is useful in controlling bleeding in the Rat kidney incision model.Öğe Effects of Algan Hemostatic Agent on bleeding time in a rat tail hemorrhage model(TURKISH ASSOC TRAUMA EMERGENCY SURGERY, 2020) Totuk, Ozgan Melike Gedar; Guzel, Sevket Ergun; Ekici, Husamettin; Kumandas, Ali; Aydingoz, Selda Emre; Yilmaz, Enis Cagatay; Kirdan, TaylanBACKGROUND: Algan Hemostatic Agent (AHA) is a multi-herbal extract containing a standardized amount of Achillea millefolium, Juglans regia, Lycopodium clavatum, Rubus caesius or Rubis fruciosus, Viscum album, and Vitis vinifera, each of which is effective in hemostasis. In this study, we aimed to investigate the effects of AHA on bleeding time in a rat tail hemorrhage model. METHODS: Forty-eight Sprague Dawley rats (5-7 weeks old, 180-210 g) were randomly and equally allocated to six groups as follows: heparin plus saline (heparinized control), heparin plus AHA-soaked sponge, heparin plus liquid form of AHA, saline (non-heparinized control), AHA-soaked sponge and liquid form of AHA. Heparin (640 IU/kg) was administered intraperitoneally three times a day for three days in heparinized groups. For the bleeding model, the tail of rats was transected. According to the study group, either saline- or AHA-soaked sponge or liquid form of AHA was applied over the hemorrhage area. In AHA- or saline-soaked sponge groups, once the bleeding time had started, it was checked every 10 seconds. If the bleeding did not stop after 40 seconds, it was accepted as a failure. In liquid AHA group, the duration of bleeding was measured using a chronometer and defined as the time (seconds) from wounding until the bleeding stopped. RESULTS: Bleeding time in the heparinized and non-heparinized control groups was over 40 seconds. After applying the sponge form of AHA on the wound area, bleeding time was significantly shortened to less than 20 seconds in both heparinized and non-heparinized rats (p<0.001 for both). The liquid form of AHA stopped bleeding in 5.0 +/- 1.2 seconds and 8.0 +/- 1.3 seconds in heparinized and non-heparinized groups, respectively. CONCLUSION: AHA is a highly effective topical hemostatic agent in a rat tail hemorrhage model, thus may provide for a unique clinically effective option for control of bleeding during surgical operations or other emergencies.Öğe Evaluation of Burn Wound Healing Efficacy and Biocompatibility of Centella asiatica Mediated Synthesised AgNPs Loaded Hybrid Nanofiber Scaffold: In Vitro and In Vivo Studies(Wiley-V C H Verlag Gmbh, 2024) Bozkaya, Ogun; Bozkaya, Esra; Ekici, Husamettin; Alcigir, Mehmet Eray; Sahin, Yasar; Cerci, Nebahat Aytuna; Karahan, SiyamiThe aim of this study is to evaluate the cell responses, potential skin reactions during the treatment process and burn wound healing efficacy of electrospun polycaprolactone/polyethylene oxide (PCL/PEO) nanofibers (NFs) containing Centella asiatica mediated synthesized silver nanoparticles (CA-AgNPs) by in vitro and in vivo studies. Apoptosis-necrosis, genotoxicity, hemolysis, and cell attachment studies are carried out within the scope of in vitro tests, and irritation, sensitivity, and burn wound studies are carried out within the scope of in vivo tests. The apoptotic index value of CA-AgNPs-[PCL/PEO] NFs material on L929 fibroblast cells is determined as 5.0 +/- 1.0% at the highest concentration and the necrotic index value is 5.0 +/- 0.3%. Micronucleus rates (%) of NFs treated with CHO (Chinese Hamster Ovary) cells are not at genotoxic level. The hemolytic index value of NFs dressing is determined as 0.23 +/- 0.03%, The primary irritation index (PII) value of NFs wound dressing is calculated as 0.36 by irritation tests. In addition, the potential sensitization reaction of NFs extract on guinea pigs is evaluated and the sensitization score is determined as 0.9. The healing efficacy of NFs material on second-degree burn wounds compared to a commercial product is supported by pathomorphological findings.Öğe Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line(ANKARA UNIV PRESS, 2020) Melekoglu, Abdullah; Ekici, Husamettin; Arat, Esra; Karahan, SiyamiMelamine and its metabolites pose health concern as they are used in various industrial products including feed and drugs. There are a limited number of studies on melamine and cyanuric acid cytotoxicity and cellular damage without a certain conclusion. The present study aimed to evaluate melamine, cyanuric acid and its combined cytotoxic effects using 3-(4.5-dimethylthiazol-2-yl) methyl thiazole tetrazolium (MTT) bromide test. The study also evaluated apoptotic and necrotic effect using a double staining method of Hoechst 33342 and propidium iodide. Melamine, cyanuric acid and their combination (1:1) were applied to L929 fibroblasts and Chinese hamster ovary (CHO) cells at various concentrations (1000 mu g/mL, 500 mu g/mL, 250 mu g/mL, 125 mu g/mL and 62.5 mu g/mL). At the highest concentration (1000 mu g/mL), the cell viability dropped down approximately to 50% both in CHO cells and L929 cells. Melamine, cyanuric acid and their mixture caused cytotoxicity in CHO cells and L929 fibroblasts in dose-dependent manner Cell death occurred through both apoptosis and mainly necrosis. Both cell types were more sensitive to the mixture of melamine and cyanuric acid and, furthermore, CHO cells were more sensitive than L929 fibroblasts. As a result, melamine, cyanuric acid and their combination caused cytotoxicity in CHO cells and L929 fibroblasts. Further studies should be conducted in different cell lines. These studies should also aim to reveal the mechanism of cytotoxicity and related pathways.Öğe Heavy metal accumulation and oxidative-antioxidative status in Angora goats depending on age(Parlar Scientific Publications (P S P), 2015) Simsek, Ozkan; Ekici, Husamettin; Cinar, Miyase; Atmaca, Nurgul; Arikan, Sevket; Guner, BayramThis study intends to designate the alterations in the oxidant-antioxidant parameters and the heavy metal concentrations based on age of Angora goats bred in Cankiri-province of Turkey. In order to conduct an analysis of serum heavy metals including aluminum (Al), chromium (Cr), iron (Fe), copper (Cu), cobalt (Co), tin (Sn), cadmium (Cd), selenium (Se), vanadium (V), manganese (Mn), zinc (Zn), lead (Pb), arsenic (As), nickel (Ni), a total of 120 blood samples were taken. Concentrations of plasma vitamin E, vitamin A and beta-carotene, concentration of malondialdehyde (MDA), activities of catalase (CAT) and superoxide dismutase (SOD) were evaluated in the erythrocytes. Correspondingly, compared to young goats, the adult goats had substantially higher levels of Al, V, Mn, Ni, As and Sn. With respect to the young goats, the vitamin E concentration and SOD activity measured in adult goats were lower and the MDA concentration was considerably higher. Consequently, it was seen that aging affected the oxidant-antioxidant parameters and heavy metal concentrations in Angora goats.Öğe Influence of Florfenicol on Hematological Parameters in Broilers(Wiley, 2018) Yigit, Ayse Arzu; Yildirim, Ebru; Baydan, Emine; Aydogan, Ilkay; Ekici, Husamettin; Guncum, Enes…Öğe Investigation of some metals in honey samples from West Mediterranean region of Turkey(Urmia Univ, 2019) Tutun, Hidayet; Kahraman, Hatice Ahu; Aluc, Yasar; Avci, Tulay; Ekici, HusamettinAnalysis of elements content in honey is important for honey quality and safety and for monitoring of environmental pollution. The levels of 22 elements, aluminum (Al), barium (Ba), calcium (Ca), cadmium (Cd), chromium (Cr), cobalt (Co), iron (Fe), copper (Cu), potassium (K), magnesium (Mg), manganese (Mn), nickel (Ni), sodium (Na), lead (Pb), strontium (Sr), silver (Ag), bismuth (Bi), gallium (Ga), indium (In), lithium (Li), thallium (Tl) and zinc (Zn), were determined in 70 samples obtained from beekeepers located in the West Mediterranean region of Turkey. Determination of elements content was carried out using ICP-OES. Chromium, Co, Cd, Ag, Bi, In and Tl were not detected in any of the tested honey samples. The most abundant metal was K which has an overall average of 764.26 mg kg(-1). Higher concentrations of Pb, Ni, Mg, Na, K and Mn were found in the samples obtained from Burdur compared to other provinces. The levels of Cu were statistically lower in Antalya in comparison to other regions. No significant differences were observed in Al, Zn, Fe, Sr, Ba, Ca and Ga levels between regions. The differences in the chemistry of honey samples collected from different regions may be due to geochemical soil composition and geographical differences. Their levels were below to the European limits and the honeys are safe for human consumption. (C) 2019 Urmia University. All rights reserved.Öğe Investigation of the effectiveness of algan hemostatic agent in bleeding control using an experimental partial splenectomy model in rats(Marmara Univ, Fac Medicine, 2019) Midi, Ahmet; Ekici, Husamettin; Kumandas, Ali; Durmus, Omercan; Bodic, Buse; Tiryaki, Mehmet; Yesilada, ErdemObjective: Algan hemostatic agent (AHA) is a plant-based hemostatic agent produced in Turkey. Although, there is a great improvement in the hemostatic technologies, more effective hemostatic products are required to be produced. The aim of this study was to demonstrate the efficacy of AHA in a partial splenectomy model in rats. In addition, in this model, postoperative abdominal adhesion was evaluated. Materials and Methods: In this study 5-7 weeks old 64 rats were used. Rats were randomly divided into 8 groups, each consisting of eight rats (4 groups heparinized and 4 groups non-heparinized). Experimental splenectomy was performed and the gauze impregnated with saline was applied to the control group for the hemorrhage control, the gauze impregnated with liquid AHA, gel and powder form of AHA, was applied to the experimental groups. Results: The time to reach complete homeostasis was significantly shorter in all AHA groups compared to the control group. The powder and the gel forms of AHA stopped the bleeding in heparinized and non-heparinized groups in 1 second. The AHA fluid (sponge) form stopped the bleeding in the first application in the control group less than 10 seconds and the second time application was not necessary. The bleeding was able to be controlled in the heparinized control group (saline impregnated sponge) by 55 seconds and in the non-heparinized control group by 38 seconds. Conclusion: This study showed that AHA is a highly effective hemostatic agent, which would be beneficial in controlling hemorrhage.Öğe Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract(Ankara Univ, 2023) Bozkaya, Ogun; Ekici, Husamettin; Gun Gok, Zehra; Arat, Esra; Ekici, Seda; Yigitoglu, Mustafa; Vargel, IbrahimIn recent years, researchers have shown an increased interest in using medicinal plants for the synthesis of silver nanoparticles (AgNPs) having various therapeutic properties. Centella asiatica (CA), a medicinal plant, has been used to treat minor burn wounds, psoriasis, and hypertrophic wounds among many other pathological conditions. The current study aimed to synthesize CA coated AgNPs (CA-AgNPs) with appropriate biocompatibility and various therapeutic properties, including antimicrobial and analgesic activities. The synthesized CA-AgNPs were characterized by ultraviolet-visible (UV-Vis) spectroscopy, zeta potential measurements, and fourier transform infrared (FT-IR) spectroscopy. The formation of spherical CA-AgNPs was confirmed by a single surface plasmon resonance (SPR) peak emerging at 420 nm wavelength by UV-Vis. The average hydrodynamic diameter and zeta potential of the particles were found to be 29.5 nm and -24.5 mV, respectively. The FT-IR analyses showed that the AgNPs were coated and stabilized by bioactive compounds from the CA extract. MTT cytotoxicity assay revealed that CA-AgNPs at <= 1 mM concentrations exhibited biocompatibility for L929 fibroblast cells. The antimicrobial activity of CA-AgNPs was confirmed by significant inhibition of Staphylococcus aureus and Escherichia coli. In addition, the analgesic effect of CA-AgNPs was investigated for the first time in the literature by tail-flick and hot plate methods, and statistically significant results were obtained for both methods. Taken together, these results suggest that CA-AgNPs can be used as an effective antibacterial and analgesic agent in a variety of biomedical applications, including coating wound dressings.Öğe The content and health risk assessment of selected elements in bee pollen and propolis from Turkey(Academic Press Inc Elsevier Science, 2022) Tutun, Hidayet; Aluc, Yasar; Kahraman, Hatice Ahu; Sevin, Sedat; Yipel, Mustafa; Ekici, HusamettinThis study aimed to determine the concentrations of potentially toxic elements (PTEs), including Al, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, Sn, V, and Zn, in pollen and propolis samples collected from migratory beekeeping areas in Turkey. A health risk assessment was performed to identify the potential risk of these PTEs to consumers in terms of public health. Concentrations of the elements were analyzed by inductively coupled plasma optical emission spectroscopy. The most abundant element in the bee pollen and propolis samples was Fe, with average concentrations of 47.3 and 390 mg kg(-1), respectively, followed by Al, Zn, Mn and Sn. Concentrations of all elements except Cu, Mn and Sn were higher in propolis than in pollen. The concentrations of Fe, Al, and Zn in the propolis samples were over eight, seven, and two times higher than in the bee pollen samples, respectively. Se, Cd, and Hg in pollen and Se and Cd in propolis were below the detection limits. Pb, Co, and Cr were detected below 0.62, 0.06, and 0.91 mg kg(-1), in pollen samples, respectively. Co and Hg were detected below 0.68, and 0.18 mg kg(-1), in propolis samples, respectively. Detection of the PTEs contamination level and assessment of the health risks in pollen and propolis is necessary to ascertain quality and safety before consumption. In this study, we concluded that bee pollen and propolis may be good indicators for the screening of environmental pollution with PTEs and standards regulating acceptable concentrations of these pollutants in bee products should be established. In addition, it has been determined that consumption at the specified rates does not pose a risk. This study suggests the determination of admissible concentrations of PTEs in bee pollen and propolis.
