Effects of Algan Hemostatic Agent on bleeding time in a rat tail hemorrhage model
Yükleniyor...
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
TURKISH ASSOC TRAUMA EMERGENCY SURGERY
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
BACKGROUND: Algan Hemostatic Agent (AHA) is a multi-herbal extract containing a standardized amount of Achillea millefolium, Juglans regia, Lycopodium clavatum, Rubus caesius or Rubis fruciosus, Viscum album, and Vitis vinifera, each of which is effective in hemostasis. In this study, we aimed to investigate the effects of AHA on bleeding time in a rat tail hemorrhage model. METHODS: Forty-eight Sprague Dawley rats (5-7 weeks old, 180-210 g) were randomly and equally allocated to six groups as follows: heparin plus saline (heparinized control), heparin plus AHA-soaked sponge, heparin plus liquid form of AHA, saline (non-heparinized control), AHA-soaked sponge and liquid form of AHA. Heparin (640 IU/kg) was administered intraperitoneally three times a day for three days in heparinized groups. For the bleeding model, the tail of rats was transected. According to the study group, either saline- or AHA-soaked sponge or liquid form of AHA was applied over the hemorrhage area. In AHA- or saline-soaked sponge groups, once the bleeding time had started, it was checked every 10 seconds. If the bleeding did not stop after 40 seconds, it was accepted as a failure. In liquid AHA group, the duration of bleeding was measured using a chronometer and defined as the time (seconds) from wounding until the bleeding stopped. RESULTS: Bleeding time in the heparinized and non-heparinized control groups was over 40 seconds. After applying the sponge form of AHA on the wound area, bleeding time was significantly shortened to less than 20 seconds in both heparinized and non-heparinized rats (p<0.001 for both). The liquid form of AHA stopped bleeding in 5.0 +/- 1.2 seconds and 8.0 +/- 1.3 seconds in heparinized and non-heparinized groups, respectively. CONCLUSION: AHA is a highly effective topical hemostatic agent in a rat tail hemorrhage model, thus may provide for a unique clinically effective option for control of bleeding during surgical operations or other emergencies.
Açıklama
Ekici, Husamettin/0000-0001-6403-737X
Anahtar Kelimeler
Algan Hemostatic Agent, bleeding time, hemorrhage, hemostasis, rat
Kaynak
ULUSAL TRAVMA VE ACIL CERRAHI DERGISI-TURKISH JOURNAL OF TRAUMA & EMERGENCY SURGERY
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
26
Sayı
6
Künye
Özgün Melike Gedar Totuk, Şevket Ergun Güzel, Hüsamettin Ekici, Ali Kumandaş, Selda Emre Aydıngöz, Enis Çağatay Yılmaz, Taylan Kırdan, Ahmet Midi. Effects of Algan Hemostatic Agent on bleeding time in a rat tail hemorrhage model. Ulus Travma Acil Cerrahi Derg. 2020; 26(6): 853-858.
