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Öğe Alterations in serum thiol-disulfiide homeostasis and ischemiamodifiied albumin concentrations in clinical canine parvoviral enteritis(Tubitak Scientific & Technological Research Council Turkey, 2024) Şenel, Yasin; Terzi, Osman Safa; Kara, Erdal; Erel, Özcan; Neşelioğlu, Salim; Ceylan, EbubekirMonitoring biomarkers related to inflammation and oxidative stress is critical in dogs because parvovirus causes both inflammatory and antioxidant alterations. The aim of this study was to investigate inflammatory and antioxidant changes caused by canine parvoviral enteritis to better understand the oxidative stress process related to this disease. Thus, the total thiol, native thiol, disulfide, and ischemia-modified albumin levels of Canine parvovirus infected symptomatic puppies and healthy puppies were examined. Using the results of complete blood counts, the blood serum thiol-disulfide homeostasis and ischemia modified albumin levels of the puppies with Canine parvoviral enteritis (n = 65) and the healthy puppies (n = 34) were compared. Canine parvoviral enteritis and control groups showed a statistically significant difference in thiol disulfide levels (p < 0.01), while no significant difference was observed in ischemia modified albumin levels between the two groups. As a result of this study, a picture contradictory to the literature information was discovered; it is believed that integrating research on oxidative stress at various stages of disease progression, including the early stage, clinical period and recovery processes may provide more information about the dynamics of oxidative stress during disease progression.Öğe Deneysel kronik toksoplazmoz fare modeli: Beyin lezyonlarının davranış değişiklikleri ile ilişkilendirilmesi(2012) Koçak, Orhan Murat; Atmaca, Hasan Tarık; Terzi, Osman Safa; Büyükkayaer, Seyhan; Rezaki, Hatice Özdemir; Uzunalioglu, Tuba; Cağdaş, Güngör DinçelAmaç: Bu çalışmada; kist oluşturan T. gondii ME 49 suşu ile enfekte farelerde oluşan anksiyete ile ilişkili davranış değişiklikleri ve beyin lezyonlarıyla ilişkisinin araştırılması amaçlanmıştır. Yöntem: Uygulama öncesi serumlarında anti-T. gondii antikorlarının olmadığı gösterilen 2 aylık 21 adet erkek Swiss albino fareden deney grubundaki 14’üne; intraperitoneal (IP) (n=8) ve oral (n=6) yollarla 2x102 Toxoplasma gondii ME49 ookisti verildikten 4 hafta sonra, anksiyete veya korku ile ilişkili davranışları T. gondii ile enfekte fareler ile kontrol grubu sağlıklı farelerde karşılaştırmalı olarak değerlendirildi. Deney bitiminde (inokulasyon sonrası 45’inci gün) farelere ötenazi yapılarak, beyinleri histopatolojik olarak ve T. gondii doku kisti antijeni yönünden immunoperoksidaz test ile incelendi. Bulgular: T. gondii ile enfekte farelerin daha düşük düzeyde anksiyeteye sahip olduklarını, enfekte olmayan kontrol grubundaki farelere göre daha yüksek oran ve süreyle açık kolu tercih ettiklerini ortaya koydu. Patolojik olarak; enfekte grupta özellikle parietal ve temporal loblar, kornu ammonis, amigdala, talamus ve çevresinde, beynin diğer bölümlerine oranla daha yüksek derecede gliozis, perivasküler hücre infiltrasyonu, meningitis ve nöron nekrozları gözlendi. Beyinde T. gondii doku kisti yerleşimi de anılan lezyon şiddeti ile doğru orantılı olarak diğer alanlara oranla daha yüksek sayıdaydı. Sonuç: Kronik toksoplazmozlu farelerin beyinlerinde şekillenen T. gondii doku kistleri ve nöropatolojik değişikliklerin doğrudan anksiyetenin azaltılmasına yönelik davranış manipülasyonu ile ilişkili olduğu öne sürülebilir.Öğe Dynamic thiol-disulphide homeostasis and ischemia modified albumin levels in neonatal calf diarrhea(Ankara Univ, 2023) Terzi, Osman Safa; Kara, Erdal; Senel, Yasin; Ceylan, Ebubekir; Neselioglu, Salim; Erel, OzcanThe aim of this study was to determine dynamic thiol-disulphide homeostasis (TDH) parameters and ischemia modified albumin (IMA) levels in calves with neonatal diarrhea and compare with healthy controls. A total of 50 calves were included in the study. There were 25 calves in both diarrhea and healthy groups. Serum native thiol, total thiol, disulfide and IMA levels were measured using new methods. Native thiol (P=0.025) and total thiol (P=0.041) values decreased significantly in calves with neonatal diarrhea compared to the healthy control group. Disulphide (P=0.133), disulphide/native thiol ratio (P=0.001) and IMA (P=0.0018) parameters were lower in healthy group, and the difference between the two groups was significant for the parameters other than disulphide. This study shows that TDH is impaired in neonatal calf diarrhea and IMA levels are increased due to oxidative stress.Öğe Experimental Chronic Toxoplasmosis Model in Mice: Brain Lesions and Related Behavioral Changes(Galenos Yayincilik, 2012) Kocak, Orhan Murat; Atmaca, Hasan Tarik; Terzi, Osman Safa; Buyukkayaer, Seyhan; Ozdemir, Hatice; Uzunalioglu, Tuba; Kul, OguzObjective: In this study, it is aimed to constitute a chronic toxoplasmosis model using toxoplasma gondii (T. gondii) ME49 strain that is supposed to produce tissue cysts, to compare healthy and infected mice in terms of behavioral changes. In addition, the relationship between behavioral changes and brain lesions was questioned. Method: Before experimental application, out of a total of twenty-one 2-month-old Swiss albino mice, T. gondii antibody-free, 14 were infected by intraperitoneal (IP) (n=8) or oral (n=6) inoculation of 2x102 Toxoplasma gondii ME49 oocysts. Four months later, fear-and anxiety-related behavioral changes in infected and healthy control groups were comparatively evaluated with plus-maze test. At the end of the experiment (45 day after inoculation), euthanasia was carried out on all mice and their brains were examined histopathologically and immunohistochemically for the presence of T. gondii tissue cysts. Results: Infected mice had low levels of anxiety, they have entered the open arms more frequently and have spent more time in the open arms of the maze compared to controls. Pathologically, in the infected group, high rates of gliosis, perivascular cell infiltration, meningitis, and neuron necrosis were observed especially in the parietal and temporal lobes, cornu ammonis, amygdala, and thalamus compared to the other parts of the brain. A higher number of tissue cyst formations were positively correlated with the lesion severity in these parts of the brain. Conclusion: It can be suggested that, tissue cysts and neuropathological changes in chronically infected mice brains have direct concern with behavioral manipulation which results from low anxiety levels. (Archives of Neuropsychiatry 2012; 49: 139-144)Öğe Immunohistochemical investigation of the damage to and repair of myelin, and astrocyte activity in small ruminants resulting from with natural meningoencephalitic listeriosis(Ankara Univ Press, 2018) Ozyildiz, Zafer; Dincel, Gungor Cagdas; Terzi, Osman Safa; Ozsoy, Sule Yurdagul; Kul, OguzMeningoencephalitic listeriosis is a significant bacterial disease in various species. Common characteristics include microabscesses in the brainstem, midbrain and cerebellum. Many aspects of damage to the central nervous system caused by the disease remain obscure. We investigated central nervous system damage by assessing the presence of biomarkers such as galactocerebroside (GAL-C), glial fibrilary acidic protein (GFAP) and myelin basic protein (MBP) in brain tissues of sheep and goats of various ages, which naturally infected with listeriosis. We examined sections of pons, medulla oblongata, rostral colliculus, cerebellum, thalamus and cornu ammonis and found increased MBP (P < 0.01), GAL-C (P < 0.01) and GFAP (P < 0.01) expression. Ihis studv showed that myelin damage in meningoencephalitic listeriosis was an important neuropathological finding. The most prominent finding of this study is the beginning of the regeneration as soon as myelin damage occurs.Öğe In-vivo efficacy of toltrazuril on experimentally induced Toxoplasma gondii tissue cysts in lambs: A novel strategy for prevention of human exposure to meat-borne toxoplasmosis(Elsevier Sci Ltd, 2013) Kul, Oğuz; Yıldız, Kader; Öcal, Naci; Freyre, Alvaro; Deniz, Abdulkerim; Karahan, Siyami; Terzi, Osman SafaThe aim of the present study was to investigate in vivo efficacy of toltrazuril on Toxoplasma gondii tissue cysts following induction of chronic toxoplasmosis in 4-week-old lambs (n = 27) by inoculation of 1 x 105 T. gondii ME 49 strain oocysts (day 0). Beginning at the 15th day after inoculation, lambs in Group T20 and Group T40 were given toltrazuril orally 2 times, once every week (Baycox 5%, Bayer Animal Health) at a dose of 20 mg/kg and 40 mg/kg, respectively. Positive control (PC) lambs were not given any therapy, and 2 clinically healthy non-infected lambs were used as negative controls (Group NC). Two out of 9 lambs in PC group (oocyst inoculated but non-treated) were killed on toltrazuril treatment days (day 15 and 22) to evaluate the tissue cyst presence in their muscles. On day 90, the remaining 25 lambs were necropsied, and samples from the brain and 11 different muscle groups were collected. The tissues were examined for the presence of tissue cysts by histopathology, immunohistochemistry, nested-PCR and percoll gradient centrifugation. Anti-T. gondii antibodies were screened by IFAT throughout the experiment. The increased T. gondii seropositivity beginning from the 15th day of inoculation remained steady at Day 45 and Day 90 in Groups PC while it was significantly lower at Day 90 in toltrazuril receiving groups. In toltrazuril treated groups, histopathological findings included degenerative changes in the cyst wall, complete macrophage invasion to the cysts, and reduction or removal of the cysts in to to. Four out of 9 lambs (44.4%) in both toltrazuril treated group (Group T20 and T40) did not contain tissue cyst in any examined tissues while all positive control animals had T. gondii tissue cysts at least in one muscle group. The toltrazuril treatment efficacy on the cyst presence was determined as 44.4%. The number of the cysts in the musculature was significantly different between non-treated and toltrazuril treated lambs (X-2 = 6.613; p = 0.037). For the total number of cysts, the positive control lambs had higher number of cysts compared to both toltrazuril treated lambs (T20 and T40) (X-2 = 5.629; p = 0.018 and X-2 = 5.629; p = 0.018, respectively) while there were no differences between Group T20 and Group T40 (X-2 = 0.000; p = 1.000). According to PCR results, the brain and M. semitendinosus were positive in all 7 control lambs while 12 out of 18 lambs were positive in toltrazuril treated lambs. In conclusion, the results are promising as the toltrazuril treated lambs had markedly less parasite counts compared to those of untreated lambs. Further research should be conducted to reveal if toltrazuril treatment in sheep could be used as a strategy to minimize the cyst exposure of humans through consumption of raw or undercooked mutton. (C) 2012 Elsevier Ltd. All rights reserved.Öğe A rare case of feline congenital Toxoplasma gondii infection: fatal outcome of systemic toxoplasmosis for the mother and its kitten(Schluetersche Verlagsgesellschaft Mbh & Co Kg, 2013) Atmaca, Hasan Tarık; Dinçel, Güngör Çağdaş; Macun, Hasan Ceyhun; Terzi, Osman Safa; Uzunalıoğlu, Tuba; Kalender, Hakan; Kul, OğuzThis report describes a case of fatal systemic toxoplasmosis in a 2.5-year-old mixed breed pregnant cat and its kittens. The pregnant cat was presented to the gynecology clinic with symptoms of dystocia. The ultrasound examination revealed the presence of five fetuses in the uterus, three of which were not alive, and consequently a cesarean section was performed. However, the mother cat and the remaining two live kittens died two and ten days after cesarean section, respectively. Pathologically, severe alveolar edema, tachyzoite-like structures in the alveolar macrophages and multifocal necroses in the lungs of mother cat were observed. An intense Toxoplasma gondii immunopositive reaction was observed in the cytoplasms of alveolar macrophages, bronchial and bronchiolar epithelia, necrotic foci in the lungs, and Kupffer cells of the liver. PCR analyses amplified T. gondii DNA in tissue samples of the mother cat and kittens. The present study provides strong evidence for a transplacental transmission of T. gondii infection with deadly outcome for the mother cat, fetuses and kittens. As to the authors' knowledge, this report is the first case of fatal congenital toxoplasmosis in domestic cats in Turkey.Öğe The effect of potassium levels on electrocardiographic data in calves with neonatal diarrhea(2024) Terzi, Osman Safa; Kara, ErdalObjective: The objective of the current study was to assess the potassium levels and electrocardiographic (ECG) data in a cohort of 40 calves (age < 30 days) diagnosed with newborn diarrhea, originating from dairy farms located in Ankara, Turkey. Materials and Methods: This study was conducted on a dairy farm located in Ankara, involving a population of 500 dairy animals. The calves were divided into two groups: one group with normal potassium levels (n=24) and another group with hyperkalemia (n=16) due to neonatal diarrhea. After the initial clinical examination was conducted on the farm, electrocardiographic investigations were carried out utilizing an ECG equipment. The biochemical analysis of blood samples that were collected in red and dry collecting tubes was carried out with the assistance of an automated analyzing machine Results: The findings indicate that there were no statistically significant differences observed across all ECG variables between the two groups. Conclusion: Acidemia and metabolic acidosis are commonly observed in calves suffering from hyperkalemic diarrhea, as documented in previous investigations, often accompanied by heart rhythm problems. In contrast, the current investigation revealed that neonatal calves with diarrhea and hyperkalemia had just a deepening of the T wave, without any additional abnormalities. Nevertheless, it is important to note that acidemia and metabolic acidosis were not of a severe nature. The implementation of Holter monitoring is indicated for calves experiencing hyperkalemia in conjunction with diarrhea.Öğe The use of two dimensional speckle tracking echocardiography in veterinary cardiology(Ataturk Universitesi, 2021) Terzi, Osman Safa; Toksoz, Cansu; Akbaba, Murat; Emrealp, GokceThe aim of this study is to present short-axis radial and circumferential strain and strain rate measurements by speckle tracking echocardiography technique in healthy cats and dogs. The study was performed at Small Animal Training and Research Hospital, Ankara University. Retrospective data from cats (n=5) and dogs (n=5), which are applied to the animal hospital for cardiological examination, were analyzed. Two-dimensional speckle tracking echocardiography was performed without sedation, and patients were manually restrained in the right and left lateral positions. Peak systolic radial strain, radial strain rate, circumferential strain, circumferential strain rate means were 24.47 ± 1.22%, 0.29 ± 0.20 s-1, -15.33 ± 2.56%, 0.05 ± 0.27 s-1 respectively for cats and were 39.33 ± 14.02%,-0.47s -1 ± 0.55 s-1, -23.13 ± 1.53%, -0.63 s-1± 0.18 s-1 respectively for dogs. It is thought that speckle tracking echocardiography can be included in routine echocardiographic examinations because this technique provides detailed information for determining the systolic potential of the left ventricle and the examination time is not much different compared to conventional echocardiographic examinations. © 2021 Ataturk Universitesi. All rights reserved.Öğe Tracking acute phase protein response during acute and chronic Toxoplasma gondii infection(Springernature, 2019) Atmaca, Hasan Tarik; Gazyagci, Aycan Nuriye; Terzi, Osman Safa; Dincel, Gungor Cagdas; Sumer, TugceToxoplasmosis is a disease caused by the protozoan Toxoplasma gondii, which occurs worldwide in mammals and birds. Brain is the primary target organ because Toxoplasma gondii is a ubiquitous intracellular parasite that causes most frequently life-threatening encephalitis in immunocompromised patients. Relation of tissue cysts number, histopathology score and acute phase proteins were investigated. In this study, 36 mice are infected with Me49 strain of Toxoplasma gondii. The control group has 6 healthy mice. After inoculation of Toxoplasma gondii, at 10., 15., 20., 30., 45., 60. days, 6 each mice euthanized after collection of blood samples. Hemopexin, haptoglobulin, macroglobulin, serum amyloid A and clusterin levels are determined by ELISA. Then, brain tissues were investigated histopathologically and lesions were scored. The average cyst numbers were determined by counting three samples (25 mu l each) of each brain homogenate under light microscopy. Inflammatory reaction was observed on day 10 days after inoculation (d.a.i.) The lesions were characterized by perivascular mononuclear cell infiltration, focal mononuclear cell infiltration in the meninges, and glial proliferation. Tissue cysts were observed in all Toxoplasma gondii-infected groups. The highest lesion score was observed at 60 d.a.i. And the most tissue cyst number were on day 30. d.a.i. Serum levels of hemopexin, haptoglobulin, macroglobulin, serum amyloid A and clusterin were significantly higher than the control group on day 10-20., 10., 10-30., 10.,10-45 d.a.i., respectively. High level of acute phase proteins in mice on certain days infected with Toxoplasma gondii was exhibited a relationship between brain lesions and tissue cysts.Öğe Yerli Kara ve İsviçre Esmeri Irkı Sığırların Kolostrum Kalitesinin Karşılaştırılması(2020) Kara, Erdal; Terzi, Osman Safa; Şenel, Yasin; Ceylan, EbubekirRuminant plasentaları sindesmokoryal tipte olduğu için maternal sirkülasyonla yavru sirkülasyonubirbirinden ayrılmaktadır. Bu sebeple yenidoğan buzağılar neonatal dönem olarak da isimlendirilendoğumu takiben ilk 28 günlük periyotta, annelerinin kolostrumundan alacakları bağışıklıkbileşenlerine ihtiyaç duyarlar. Pasif immun transfer başarısı için toplam kolostralImmunlobülinlerinlerin %85-90’ını oluşturan Immunglobülin G’nin kolostrumdaki miktarı kalitenoktasında belirleyicidir. Çalışmanın amacı; yerli gen kaynaklarımızdan olan Yerli Kara ırkı sığırlarile kombine verimli bir kültür ırkı olan İsviçre Esmeri’nin kolostrum kalitelerinin karşılaştırılmasıdır.Çalışmanın hayvan materyalini 40 saf Yerli Kara sığır ve 39 saf İsviçre Esmeri sığır oluşturdu.Kolostrum Immunglobulin G seviyesi ELISA yöntemi kullanılarak belirlendi. Yerli Kara ve İsviçreEsmeri sığırların tanımlayıcı istatistik verilerine göre ortalama Immunglobulin G konsantrasyonlarısırasıyla; 133.1±77.28 mg/mL, 74.60±28.44 mg/mL olduğu tespit edildi. İki grup arasındakiImmunglobulin G verileri bağımsız t testi ile karşılaştırıldığında istatistiksel olarak önemli olduğutespit edildi (P<0.001). Sonuç olarak; bu çalışmayla Yerli Kara ırkının kolostrum kalitesinin sütüretimi yüksek olan İsviçre Esmeri ırkına oranla oldukça yüksek olduğu görüldü.
