DSpace Arşivi :: by Yazar "Yildiz, Kader" değerine göre listeleniyor

Yazar "Yildiz, Kader" seçeneğine göre listele

Listeleniyor 1 - 20 / 28

Ceratothoa oestroides (Risso, 1826) (Isopoda: Cymothoidae) in black seabream from the Northern Aegean Sea
(2009) Gökpinar, Sami; Ozgen, Ediz Ka?an; Yildiz, Kader
Ceratothoa oestroides (Risso, 1826), is an ectoparasite found in a variety of fish species. This parasite was detected on caudal pedoncule of black seabram (Spondyliosoma cantharus) hunted in Balikesir. This is the first report of C. oestroides in the black seabram (Spondyliosoma cantharus).
Crossroad between the sylvatic and domestic cycles of Mesocestoides litteratus: mesocestodiasis in dogs adopted from shelters in Türkiye
(Springer, 2024) Akdeniz, Sinem; Akkus, Gozde Nur; Avci, Beyza; Gazyagci, Aycan N.; Yildiz, Kader
This study aimed to report the presence of Mesocestoides litteratus in dogs adopted from shelters in T & uuml;rkiye. Gravid segments were examined microscopically in the faeces of dogs from different shelters located in Ankara and Kirikkale provinces in the central region of T & uuml;rkiye. Then, genomic DNA obtained from these segments, a 446-bp fragment of the mitochondrial cytochrome C oxidase subunit 1 gene, and a 350-bp fragment of mitochondrial 12S rRNA were amplified and sequenced. BLASTn search was performed. During light microscopic examination, an egg-filled paruterine organ was observed in the middle part of the segment. Thin-shelled, oval, 35-mu m-diameter parasite eggs containing an oncosphere with three pairs of hooklets were observed. The gravid segments were determined as Mesocestoides spp. based on the appearance of the typical paruterine organ. PCR results supported our diagnosis; moreover, according to the BLAST results, it was detected that the species infecting two dogs was 98.01-100% similar to M. litteratus. Praziquantel-containing medication was administered to the infected dogs at a dosage of 5 mg/kg. Foxes act as the final host of M. litteratus and the parasite is prevalent in wildlife; however, these animals may disperse the parasite in urban life. Veterinarians need to be made more aware of this parasite, especially if the dogs are owned from shelters.
Cystocaulus ocreatus infection in Anatolian wild sheep and dwarf goats
(Scientific Technical Research Council Turkey-Tubitak, 2007) Kabakci, Nalan; Yildiz, Kader; Duru, Sibel Yasa; Yarim, Murat
An Anatolian wild sheep (Ovis orientalis anatolica) from a wild ruminant farm near Kirikkale, Turkey, was necropsied. Parasitic nodules were situated beneath the pleura of the caudodorsal lobes of the lungs. First stage larvae of Cystocaulus ocreatus were detected both in bronchial fluid and in lung paraffin sections. Clinical and parasitological examinations of the other animals (4 dwarf goats and 3 wild sheep reared on the same farm) were also performed. First stage larvae of C. ocreatus, and eggs of Nematodirus spp. and Strongyloides spp. were observed in coproscopic examination. Anthelmintic treatment, Rabenzole (R) (rafoxanide and thiabendazole, Topkim), was administered to the animals infected by parasites. At the end of the treatment the combination of rafoxanide and thiabendazole was found to be markedly effective for the treatment of lungworm infection and no parasitic eggs were detected in the faeces of any of the treated animals.
Detection and isolation of Leishmania infantum from natural infected dog in Türkiye
(Elsevier, 2025) Damlapinar, Aysegul; Berberoglu, Umut; Mungan, Mesut; Celebi, Bekir; Gazyagci, Aycan N.; Yildiz, Kader
Some clinical signs such as cachexia, alopecia, exfoliative dermatitis, hair loss, and swollen lymph nodes were observed in a one-year-old crossbred male dog living in a rural area of Kirikkale province, T & uuml;rkiye, was presented to the veterinary clinic by its owner. Anaemia, leucopenia, hyperglobulinemia, and bilirubinemia were detected. Seropositivity was detected using Leishmania IgG/IgM Rapid Test. The amastigote forms of the parasite were observed in the lymph node aspirates. Viable promastigotes were observed in the samples obtained from the Novy-MacNeal-Nicolle medium (NNN) tubes. The parasite DNA was extracted from the promastigotes produced in the NNN medium using a DNA extraction kit according to the manufacturer's instructions. The specific bands indicating the gene regions of L. infantum (350 bp and 730 bp for HSP20 and HSP70, respectively) were observed. Additionally, BLASTn analysis revealed 100 % similarity with GenBank-deposited L. infantum sequences. The sequences were submitted to GenBank (Accession numbers: OR806945 and OR806946). According to the author's knowledge, it is the first dog isolate deposited in the collection of the Microbiology Reference Laboratories and Biological Products Department, General Directorate of Public Health, Ministry of Health, Ankara, T & uuml;rkiye. The isolate (TR_L. infantum CanL-1_damla) has been preserved by cryopreservation at -150 degrees C for further studies.
Determination of extracellular traps structures from sheep polymorphonuclear leukocytes to Echinococcus granulosus protoscoleces
(Academic Press Inc Elsevier Science, 2022) Yildiz, Kader; Simsek, Neslihan Sursal; Gurcan, I. Safa
It was aimed to detect extracellular traps structures from sheep polymorphonuclear leukocytes (PMN) after being confronted with Echinococcus granulosus protoscoleces in vitro. Also, the effect of cyst fluid was examined on the development of extracellular traps. At the end of the incubation for 1 h, the extracellular traps augmented with neutrophil elastase, histone (H3) and myeloperoxidase were visualized in the protoscoleces-PMN co-culture microscopically. Some protoscoleces lysed and the chitinous hooks released were surrounded by the extracellular traps. The other protoscoleces were still intact and the extracellular trap structures were observed around them. The relationship between the extracellular DNA contents and the protoscoleces concentration was not found statistically significant (P > 0.05). The extracellular DNA amount in the co-cultures diluted in RPMI-1640 increased with the incubation time (P < 0.05). However, the time-dependent relationship was not found in the co-cultures diluted in the cyst fluid (P > 0.05). The difference in the extracellular DNA amount was detected as statistically significant (P < 0.05) between the two co-culture groups (diluted in RPMI-1640 or the cyst fluid), except for 30 min incubation. To the Author's knowledge, NETosis reaction was firstly observed in sheep PMN after being confronted with protoscoleces in vitro. The cyst fluid had some negative effects on the development of extracellular traps from sheep PMNs at the 1-h incubation time. It should be investigated which molecules are responsible for NETosis inhibition in hydatid cyst fluid. Future studies may clarify whether neutrophils fight with protoscoleces by using their different mechanisms.
Development and validation of SYBR Green- and probe-based reverse-transcription real-time PCR assays for detection of the S and M segments of Schmallenberg virus
(SAGE PUBLICATIONS INC, 2020) Azkur, Ahmet Kursat; van der Poel, Wim H. M.; Aksoy, Emel; Hakze-van der Honing, Renate; Yildirim, Murat; Yildiz, Kader
Schmallenberg virus (SBV), discovered in Germany in 2011, causes congenital malformations in ruminants. Reverse-transcription real-time PCR (RT-rtPCR) assays based on various segments of SBV have been developed for molecular detection. We developed alternative RT-rtPCR assays for SBV detection to avoid earlier reported mutations and hypervariable regions of the S and M segments of the viral genome. For SYBR Green-based detection of the S segment, theR(2)value and efficiency of the developed assay were 0.99 and 99%, respectively. For probe-based S segment detection, 2 assays were developed; the first had anR(2)value of 0.99 and 102% efficiency, and the second had aR(2)value of 0.98 and 86% efficiency. The probe-based M segment assay had anR(2)value of 1.00 and 103% efficiency. Detection limits of the RT-rtPCR assays with new primer sets were 10(2)and 10(1)copies/mu L for the S and M segments, respectively. Field samples from cattle and sheep were also used for primary validation of the developed assays. Our assays should be suitable for SBV detection in ruminants and for in vitro studies of various SBV strains.
Extracellular Trap Formation by Donkey Polymorphonuclear Neutrophils Against Toxoplasma gondii
(Elsevier Science Inc, 2019) Yildiz, Kader; Gokpinar, Sami; Sursal, Neslihan; Babur, Cahit; Ozen, Dogukan; Azkur, Ahmet Kursat
Toxoplasma (T.) gondii is an obligatory intracellular apicomplexan parasite. The donkey is one of the intermediate hosts of T. gondii. There are almost no data about T. gondii infection in donkeys, apart from a few seroprevalence reports. The aim of the study was to detect the formation of extracellular traps by donkey polymorphonuclear neutrophils (PMNs) when exposed to T. gondii tachyzoites in vitro. Tachyzoites were observed to be entrapped within extracellular traps (NET) structures. Myeloperoxidase, neutrophil elastase (NE), and histone (H3) were observed in NET structures. NE and reactive oxygen species activity increased with time and was higher in the PMN-tachyzoite cocultures. Some tachyzoites were seen close localization to the nucleus of neutrophils. Degranulation and extracellular traps were observed simultaneously in some donkey neutrophils after incubation for 1 hour. The tachyzoite invasion rate decreased in PMN-tachyzoite cocultures in comparison to the controls. NETs can negatively affect the viability of entrapped tachyzoites in addition to their action of physical entrapment. NE may play a key role in the viability of T. gondii in donkeys. The amount of extracellular DNA increased with incubation time in the PMN-tachyzoite cocultures (P < .001). This is a first report regarding the formation of donkeys NETs after exposure to T. gondii tachyzoites in vitro. Unlike sheep and cattle PMN, degranulation and extracellular traps developed in the same neutrophil. Further studies focusing on signaling pathways may shed light on what determines the behavior of donkey neutrophils after exposure to T. gondii. (C) 2018 Elsevier Inc. All rights reserved.
Extracellular traps development in canine neutrophils induced by infective stage Toxocara canis larvae
(Elsevier, 2024) Akkus, Gozde Nur; Yildiz, Kader
Neutrophils, a crucial element of the host defense system, develop extracellular traps against helminth parasites. Neutrophils accumulate around the larvae of Toxocara canis (T. canis) in the tissues of the organism. This study aimed to determine the reaction in canine neutrophils after incubation with infective stage T. canis larvae (L3) in vitro. Most L3 were still active and moved between the extracellular traps (NETs) after 60-min incubation. NETs were not disintegrated by L3 movement. The L3 was only immobilized by NETs, entrapped larvae were still motile between the traps at the 24 h incubation. NETs were observed not only to accumulate around the mouth, excretory pole or anus but also the entire body of live L3. The extracellular DNA amount released from the canine neutrophils after being induced with phorbol 12-myristate 13-acetate was not affected by T. canis excretory/ secretory products obtained from 250 L3. To the Authors'knowledge, the extracellular trap structures was firstly observed in canine neutrophils against T. canis L3 in vitro. NETs decorated with myeloperoxidase, neutrophil elastase and histone (H3) were observed under fluorescence microscope. There were not significant differences in the amount of extracellular DNA (P > 0.05), but the morphological structure of NETs was different in the live and head-inactivated T. canis larvae.
Feline Giardiasis In Turkey: Prevalence And Genetic And Haplotype Diversity Of Giardia Duodenalis Based On The Beta-Giardin Gene Sequence In Symptomatic Cats
(ALLEN PRESS INC, 2020) Sursal, Neslihan; Simsek, Emrah; Yildiz, Kader
Giardia duodenalis is a common zoonotic protozoan parasite with a broad host distribution. The main objectives of the present study were to determine the prevalence of giardiasis and to reveal the genetic and haplotype diversity of G. duodenalis in symptomatic cats in Turkey. Fecal samples were collected from cats (n = 102) with diarrhea that were admitted to different pet clinics in the Central Anatolia region of Turkey. All samples were analyzed by microscopic examination (ME), rapid immunochromatographic test (ICT), and PCR targeting the beta-giardin (bg) loci of the parasite. Phylogenetic, haplotype, and network analyses of G. duodenalis based on the bg gene were carried out. Overall, G. duodenalis was detected in 70/102 (68.6%) of the cats with diarrhea by ME (38/102, 37.3%), ICT (51/102, 50%), and PCR (30/102, 29.4%). According to sequence analyses of the bg gene region, all isolates were identified as G. duodenalis assemblage B. Haplotype analyses revealed 2 known and 8 novel haplotypes for G. duodenalis assemblage B. This study provides first prevalence and genetic and haplotype diversity data on G. duodenalis assemblage B from cats in Turkey.
The first report of Eucoleus dispar (Nematoda: Capillariidae) in the common buzzard (Buteo buteo) in Turkey
(Scientific Technical Research Council Turkey-Tubitak, 2015) Yildiz, Dincer; Buyukcopur, Emine Umran Bozkurt; Gultiken, Murat Erdem; Yildiz, Kader
A common buzzard (Buteo buteo) was brought to the Department of Anatomy, Kirikkale University Faculty of Veterinary Medicine, following its death from trauma. The tongue of the buzzard was processed for routine histology and stained with hematoxylin and eosin. A curled Eucoleus dispar and its eggs, embedded in the dorsal epithelium of the tongue, were observed in histological sections during morphological analysis of the papillae lingua in the buzzard under a light microscope. According to the authors' knowledge, this is the first report on E. dispar in the buzzard in Turkey.
Genotyping of Echinococcus granulosus obtained from cattle slaughtered in Kirikkale Province
(Ankara Univ Press, 2017) Gokpinar, Sami; Degirmenci, Rukiye; Yildiz, Kader
Strain differences are responsible from some important differences such as host specifity, growth rate, antigenic properties, sensitivity to chemotherapeutic agents, transmission dynamics and epidemiological variations in Echinococcus granulosus. In this study, it was aimed to detection strain identification of E. granulosus obtained from liver and lungs of cattle slaughtered in Kirikkale region. For this aim, mitochondrial cytochrome c oxidase subunit 1 (COI) and 12S rRNA genes of E. granulosus isolates amplified by specific primers and then COI gene sequence was made. In the present study, the isolates were identified as G1-G3 complex according to COI sequencing results.
In vitro investigation of NETosis reaction developing from dog polymorphonuclear neutrophils to Toxoplasma gondii
(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2020) Karakurt, Gunes; Yildiz, Kader
Toxoplasma gondii is known to develop extracellular traps from neutrophils in some animal species such as mice, cattle, sheep, cats, and donkeys. This study aimed to investigate the extracellular trap structures formed in dog polymorphonuclear leukocytes (PMNs) to T. gondii tachyzoites in vitro. Dog PMN was isolated using Percoll dilutions (45%, 54%, 63%, and 72%). After incubation with tachyzoites, the extracellular traps originating from the dog PMNs were observed in the extracellular areas. Histones (H3), myeloperoxidase (MPO), and neutrophil elastase (NE), the characteristic features of NETosis reaction, were detected in extracellular areas. The tachyzoites were observed between the extracellular trap structures. A positive correlation was detected between the parasite concentration and extracellular traps formation but they were not statistically significant (P > 0.05). Time-dependent relationships were also not statistically significant (P > 0.05). The extracellular traps released in the PMN-tachyzoite culture increased until the 60th min of incubation. Reactive oxygen species, MPO, and NE activities were observed in the PMN-tachyzoite culture during the incubation period. The development of extracellular traps against T. gondii in dog PMNs is reported for the first time in this study. However, it could not be determined whether the extracellular traps released from dog PMNs only mechanically immobilize or have some lethal effect on tachyzoites.
In vitro investigation on extracellular traps formation of cat polymorphonuclear leucocytes against Toxoplasma gondii
(Tubitak Scientific & Technological Research Council Turkey, 2021) Simsek, Neslihan Sursal; Cakmak, Ayse; Yildiz, Kader
Neutrophil granulocytes are one of the most important defenders of the innate immune system in the host. Moreover, neutrophils are able to reach the inflammation area and kill the pathogens using various defense strategies including neutrophil extracellular traps (NETs). However, there is still not enough information available regarding the innate immunity against Toxoplasma gondii in cats that are both definitive and intermediate hosts of this parasite. Therefore, we investigated the in vitro NETs formation which is induced by cat polymorphonuclear leucocytes (PMNs) against T. gondii tachyzoites. Firstly, PMNs were isolated from cat venous blood samples by using discontinues Percoll dilutions (72%, 63%, 54%, and 45%). Afterward, MN-tachyzoites cocultures were stained against histone (H3), neutrophil elastase (NE), and myeloperoxidase (MPO) by using monoclonal antibodies and were examined under a fluorescence microscope. The effect of different parasite doses (1:1, 1:3, and 1:5) and incubation times (30, 60, 90, and 120 min) on NETs formation was also evaluated. The presence of the extracellular DNA content was measured using a fluorometer. Confluent Vero cell cultures were used to assess the effect of NETs on the tachyzoites viability. The classical structures of NETs, such as extracellular DNA, NE, H3, and MPO were microscopically observed in the NETs formation released from cat PMNs. The amount of extracellular DNA increased in parallel with the incubation time (p < 0.001). The influence of the tachyzoites dose on the NETs formation was not statistically significant (p > 0.05). Zymosan was used as a positive control in the experiments and it was shown to be an important inducer for the NETs formation. In conclusion, as mentioned previous studies and considering our results, the NETs may be a conserved strategy to control T. gondii infection in hosts because of the immobilization and lethal effects.
Investigation of Neospora caninum tissue cysts in cattle
(Ankara Univ Press, 2017) Yildiz, Kader; Gokpinar, Sami
In this study, it was aimed to detect N. caninum seroprevalence in 200 cattle randomly selected and to detect tissue cysts of N. caninum in tissue samples of brain and skeletal muscles of the seropositive cattle. Seropositivity of N. caninum was detected in 5.5% of cattle (11/200). N caninum tissue cysts were not observed in tissue samples of cattle examined with percoll gradient centrifugation. However, N. caninum DNA was amplified in brain tissues of two seropositive bulls (18.1%).
The Molecular Characterization of Echinococcus granulosus Isolates from Sheep in Kirikkale Region
(Kafkas Univ, Veteriner Fakultesi Dergisi, 2010) Yildiran, F. Azize Budak; Yildiz, Kader; Cakir, Suekran; Gazyagci, Aycan N.
In present study, it was aimed to determine molecular characterization of Echinococcus granulosus obtained from sheep. For this purpose, DNA isolation was done from germinal membrans and protoscolices of 24 hydatic cyst samples obtained from different organs of infected sheep and then DNA samples were scanned by RAPD-PCR using 16 primers. Echinococcus granulosus G1 strain was used as reference material. Only 4 of these 16 primers (AP2, AP4, OPB12 and G05) gave the typical RAPD band patterns in both sheep isolates and the reference G1 strain. The single band of 250 bp occurred by AP2 primer was common in both sheep isolates and G1strain According to result, AP2 primer can be successfully used for separation of G1 strain in different isolates. The results indicated that all sheep isolates sampled in this study can be assumed in the same Echinococcus granulosus strain.
Morphological and ecological features of some nematodes of the genus Rhabdochona in marinka obtained from Fergana Valley, Uzbekistan
(Springer, 2021) Kuchboev, Abdurakhim E.; Najmidinov, Eldor Kh; Mukhamediev, Muzaffar A.; Karimova, Rokhatoy R.; Yildiz, Kader
The aim is to study the morphological and ecological features of some nematode species of the genus Rhabdochona parasites of marinka fish in the Fergana Valley, Uzbekistan. Rhabdochona gnedini, Rhabdochona denudata and Rhabdochona hellichi turkestanica were detected as 5.3%, 7.9% and 3.7%, respectively. According to the Authors’ knowledge, Rh. hellichi turkestanica was recorded in Uzbekistan and Central Asian republics for the first time. Rhabdochona spp. were only detected in the intestine of marinkas lived in the mountain rivers Rezaksay, Chodaksay and small tributaries of the Syrdarya river in western Fergana. The prevalence of Rhabdochona spp. in the marinka can be associated with a wide range of nutrition in the mountain rivers and small tributaries of the Syrdarya river, where it could eat large numbers of mayfly and caddis larvae that act as intermediate hosts of rhabdochona. © 2021, Indian Society for Parasitology.
Neutrophil isolation from feline blood using discontinuous Percoll dilutions
(Georg Thieme Verlag Kg, 2018) Sursal, Neslihan; Cakmak, Ayse; Yildiz, Kader
Objective: Some studies have performed in vitro neutrophil isolation from feline blood. The major limiting factor for these studies is the small volume of blood that can be collected without development of potentially life-threatening complications. In the present study we attempted neutrophil isolation from feline venous blood samples using discontinuous Percoll gradients. Material and methods: Blood was collected from the cephalic vein of clinically healthy adult cats. The blood samples were layered on Percoll dilutions (72 %, 63%, 54% and 45%). After centrifugation, the feline polymorphonuclear leukocytes (PMN) accumulated as a band between 72-63% Percoll dilutions. The total cell count was calculated using light microscopy counts. The percentage of the neutrophils was determined microscopically after staining with Diff-Quik stain. Neutrophil viability was evaluated with a 0.01 % Trypan blue assay. The activation was determined based on intact cell morphology in the isolated neutrophils. Results: The mean PMN number was 22 x 10(5) per ml (minimum-maximum: 20-26 x 10(5)ml). Neutrophil homogeneity was > 95% in the cell suspensions. The viability of isolated neutrophils was > 98%. The technique did not result in neutrophil activation. Conclusion and clinical relevance: Discontinuous Percoll gradients (72%, 63 %, 54% and 45%) can be used to isolate neutrophils from blood samples of cats. The technique was simple to perform and neutrophil activation was minimal.
A new approach to Neospora caninum infection epidemiology: neosporosis in integrated and rural dairy farms in Turkey
(Scientific Technical Research Council Turkey-Tubitak, 2014) Ocal, Naci; Atmaca, Hasan Tarik; Albay, Metin Koray; Deniz, Ahmet; Kalender, Hakan; Yildiz, Kader; Kul, Oguz
Both cattle and dogs were examined in modern and rural dairy farms that had a history of abortion over 5%. The blood samples were collected from 427 aborted cattle and the sera were tested using a commercial ELISA test kit. Additionally, a necropsy procedure was carried out on the fetuses and calves dead within 2 months after birth; the tissue samples were evaluated by histopathologic, immunoperoxidase, and PCR techniques. Eighteen dogs in close contact with the cattle in the same field were included in the study and blood and feces samples were collected. The feces samples were analyzed by copro-PCR and the sera were tested by indirect fluorescent antibody test. As a result, 161 out of 427 sera samples (37.7%) were found positive for N. caninum. In cattle, the lowest seropositivity was 6.7% and the highest seropositivity was 74.24%. Neosporosis seroprevalence in integrated holdings was lower than those of rural dairy cattle facilities (66.7%). The seropositivity for N. caninum in dogs was determined as 72.7% in rural holdings and 28.6% in integrated holdings. According to the risk analysis, N. caninum-seropositive cows had greater exposure to N. caninum-seropositive dogs in rural family holdings and integrated holdings (P = 0.054, odds ratio = 0.929; and P= 0.008, odds ratio = 0.986, respectively).
Occurrence and First Molecular Characterization of Cryptosporidium felis in a Cat in Turkey
(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2020) Sursal, Neslihan; Simsek, Emrah; Yildiz, Kader
Cryptosporidium species are highly prevalent and significant zoonotic protozoan parasites that cause severe diarrhea in many hosts. To date, cryptosporidiosis has been molecularly reported from humans and different animal hosts in Turkey. The aim of this case report is to reveal the molecular characterization based on small subunit (SSU) rRNA gene sequence of Cryptosporidium oocysts that were microscopically found in a three-month age female cat with severe diarrhea. SSU rRNA gene of Cryptosporidium was amplified for sequence and phylogenetic analyses. According to sequence analysis, Cryptosporidium spp. oocysts were characterized as C. felis (isolate name: ANK_1; accession number: MN394123). ANK_1 isolate showed a range from 99.1% to 100% identity with the most of C. felis sequences available in GenBank. However, it also exhibited high genetic distance (1.5-4.3%) with some of C. felis sequences. The intraspecific genetic differences among all SSU rRNA sequences of C. felis isolates included in the data set were determined ranged from 0.0% to 6.9%. As a conclusion, we provided the first microscopic and molecular evidence of the occurrence of C. felis in a house cat in Turkey. Although C. felis has a relatively low zoonotic risk to public health when compared with C. parvum, infected cats should not be neglected for zoonotic transmission due to close relationships between cats and humans.
Optimisation of Indirect ELISA by Comparison of Different Antigen Preparations for Detection of Antibodies Against Schmallenberg Virus
(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2020) Azkur, Ahmet Kursat; Aksoy, Emel; Yildirim, Murat; Yildiz, Kader
Schmallenberg virus (SBV) infection, discovered in 2011, was reported in Europe including Turkey, Africa and recently in some Asian countries. Commercial enzyme-linked immunosorbent assay (ELISA) kits were widely used by researchers in many epidemiological studies and SBV diagnosis. The aim of this study was to optimise indirect in-house ELISA that is based on different antigen preparations of cell-culture derived whole SBV particle. Antigen preparations were maintained with various methods: PEG precipitation, ultracentrifugation, dialysis, and antigen inactivation. Following antigen optimisation, steps of antigen coating, blocking, conjugate and stop solution were optimised and in-house ELISA was compared to commercial indirect SBV ELISA kit. The best result in ELISA antigen preparation for SBV was gained by 30% PEG purification method followed by formaldehyde inactivation. Although results of this study demonstrated that in-house ELISA for detection of SBV specific antibodies was equally sensitive and specific as commercial kit, purified SBV antigen based in-house ELISA development could increase S/P ratios.

Yazar "Yildiz, Kader" seçeneğine göre listele

Sayfa Başına Sonuç

Sıralama seçenekleri