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Two new species of Zodarion Walckenaer, 1826 (Araneae: Zodariidae) from Turkey
(Magnolia Press, 2021) Coşar, İlhan
Two new species of the megadiverse genus Zodarion Walckenaer, 1826, from the Aegean and Southeastern Anatolian regions of Turkey are described and illustrated: Zodarion bozdagensis spec. nov. (male,female) from Izmir and Zodarion siirtensis spec. nov. (male) from Siirt. This contribution increases the total number of Zodarion species to 23 for Turkey. The distribution of these species in Turkey is also mapped.
The role of Bax/Bcl-2 and Nrf2-Keap-1 signaling pathways in mediating the protective effect of boric acid on acrylamide-induced acute liver injury in rats
(Pergamon-Elsevier Science Ltd, 2022) Cengiz, Mustafa; Ayhancı, Adnan; Akkemik, Ebru; Şahin, İlknur Kulcanay; Gür, Fatma; Bayrakdar, Alpaslan; Cengiz, Betül Peker
Introduction: This study aims to investigate whether boric acid (BA) can protect rats from acrylamide (AA)induced acute liver injury. Materials and methods: AA was used to induce acute liver injury. Thirty rats were divided into five group including Group 1 (saline), Group 2 (AA), Group 3 (20 mg/kg BA), Group 4 (10 mg/kg BA+AA) and Group 5 (20 mg/kg BA+AA). Their blood and liver were harvested to be kept for analysis. Liver function enzyme activities were performed by spectrophotometric method. Catalase (CAT), superoxide dismutase (SOD) activity, and malondialdehyde levels were determined by colorimetric method. The in-silico studies were performed using the blind docking method. Results: Administration AA to rats, biochemical parameters, liver histology, and expression levels of apoptotic markers were negatively affected. However, after the administration of BA, the altered biochemical parameters, liver histology, and expression levels of apoptotic markers were reversed. Moreover, the mechanisms of AA-induced deterioration in the levels of SOD, CAT, and Nrf2-Keap-1 and the mechanisms of the protective effect of BA against these deteriorations were explained by in silico studies. Conclusion: Thus, the present study could explain the interactions between AA and thiol-containing amino acid residues of Keap-1, the effect of BA on these interactions, and the biochemical toxicity caused by the AA. In this sense, this work is the first of its kind in the literature. Based on the biochemical, histopathological, and in silico results, it can be suggested that BA has the potential to be used as a protective agent against AA-induced liver injury.
Nutrition Literacy and Quality of Life of University Students: Evidence from a Cross-Sectional Survey
(Mashhad Univ Med Sciences-Iranian Assoc Health Educ & Health Promotion, 2024) Ceylan, Jiyan Aslan; Gümüş, Aylin Bayındır
Background and Objectives: Nutrition literacy is a concept that improves individuals' diet quality, and quality of life and contributes to enhancing their general health by using and interpreting nutrition-related information correctly. This study purposed to determine the nutritional literacy and quality of life levels of university students and relate them with anthropometric measurements. Materials and Methods:This is a cross-sectional study and was conducted with 374 university students over the age of 18 at a state university. Nutrition literacy was assessed by using the Evaluation Instrument of Nutrition Literacy on Adults (EINLA), and quality of life was evaluated with the World Health Organization's Quality of Life Questionnaire (Turkish_WHOQOL-BREF). Waist, middle-upper arm, neck circumference, body weight, and height were measured in accordance with appropriate techniques by researchers. Independent t and One-Way ANOVA tests were used to determine differences between two and more than two mean values. Pearson correlation analysis was used to detect relationships between scores of total EINLA, its sections, total Turkish_WHOQOL-BREF and its dimensions, and other variables. Results: The mean EINLA and WHOQOL-BREF scores of the participants were 25.3 +/- 4.7 (borderline) and 54.3 +/- 15.1, respectively. While the EINLA score varied according to gender, faculty (health sciences vs others), and degree (1 to 4), the WHOQOL-BREF score varied according to gender and living place (home vs dormitory). EINLA and WHOQOL-BREF scores of BMI (underweight to obese), waist circumference, and neck circumference (normal vs at risk) groups were not different. It was found positive significant relationships between the second section of EINLA (reading comprehension and interpretation) and psychological health, the third section of EINLA (food groups), and social relationships (p<0.05). Conclusion: Nutrition literacy, anthropometric measurements, and quality of life of university students are affected by a multifactorial situation.
Novel Tricyclo[4.2.1]Nonane and Bicyclo[2.2.1]Heptane Derivatives: Synthesis, in Vitro Biological Activities and in Silico Studies
(Wiley-V C H Verlag Gmbh, 2024) Sarıkaya, Meryem Keçeci; Yağlıoğlu, Ayşe Şahin; Ceylan, Mustafa; Yırtıcı, Ümit
The target benzothiazole derivatives (8a-g) were synthesized starting from norbornene. The addition of dichloroketene to norbornene followed by the reduction of chlorine atoms were synthesized tricyclo[4.2.1.02,5]non-7-en-3-one (4). The condensation of benzaldehyde derivatives with compound 4 were obtained chalcone analogs (6a-g). Finally, benzothiazole derivatives (8a-g) were obtained by the reaction of the chalcone analogs with 2-aminobenzothiol in an acidic medium. The antiproliferative activities of compounds 6a-g and 8a-g were determined against C6 (rat brain tumor) and HeLa (human cervical carcinoma) cell lines using a BrdU cell proliferation ELISA assay with 5-fluorouracil (5-FU) as a standard. In both series, when compared with 5-FU (IC50=3.8 mu M for C6 and 16.33 mu M for HeLa), the most active compounds against C6 cells were 6a and 8g with IC50 values of 14.13 mu M and 29.99 mu M, respectively. With this, 6a, 6e, 6f, and 8b were the most active compounds against HeLa cells with IC50 values of 0.8, 1.21, 19.33 and 18.13 mu M, respectively. Additionally, the SwissADME online web tool was used to predict the physicochemical and ADME properties of the tested compounds. The results showed that all compounds possess promising predicted physiochemical and pharmacokinetic properties and comply with Lipinski's rule of 5, indicating that they are predicted to be orally bioavailable that they possess a predicted bioavailability score of 0.55. Furthermore, in the SwissADME Boiled-Egg chart, all compounds showed high predicted GIT absorption, while compounds 6a-g showed blood-brain barrier (BBB) permeation, and compounds 8a-g did not. Moreover, none of the compounds was (P-gp) substrates. This study investigated the potential interactions between the antiapoptotic proteins Bcl-2, Bcl-xl, Bcl-w, Brag-1, Bfl-1, and Mcl-1 and compounds 6a, 8b, and 8g through molecular docking studies. The findings suggest that these compounds may effectively inhibit antiapoptotic proteins, as evidenced by significant hydrogen bonds and hydrophobic interactions, particularly with Bcl-xl.
Notes on the genus Stalagtia from Turkey with description of a new species (Araneae, Dysderidae)
(Magnolia Press, 2023) Coar, Lhan; Danışman, Tarık; Yağmur, Erşen Aydın; Kunt, Kadir Boğaç
[Abstract No tAvailable]
New Diacetic Acids Containing Quinazolin-4(3H)-one: Synthesis, Characterization, Anticholinergic Properties, DFT Analysis and Molecular Docking Studies
(Wiley-V C H Verlag Gmbh, 2023) Tokalı, Feyzi Sinan; Sağlamtaş, Rüya; Öztekin, Aykut; Yırtıcı, Ümit; Çomaklı, Veysel
In this study, a new series of quinazolin-4(3H)-ones, which constitute an important part of biologically active heterocyclic compounds, were synthesized with excellent yields (99-94 %). The structures of the synthesized compounds (1-14) were characterized with Fourier-transform infrared (FTIR), nuclear magnetic resonance (H-1 NMR - C-13 NMR), and high-resolution mass spectroscopy (HRMS). Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition properties were examined to evaluate the anticholinergic properties of the synthesized compounds. For AChE, molecules showed IC50 in ranging of 16.27-9.12 mu M and K(i)s in ranging of 19.20 +/- 0.68-4.83 +/- 0.19 mu M. For BChE, molecules showed IC50 in ranging of 16.77-8.50 mu M and K(i)s in ranging of 10.35 +/- 2.15-3.38 +/- 0.25 mu M. Molecular docking was performed to determine the predicted interactions between the synthesized molecules and enzymes. Additionally, Density-functional theory (DFT) studies were also carried out to clarify the electronic structures of compounds.
Natural flavonoids as promising lactate dehydrogenase A inhibitors: Comprehensive in vitro and in silico analysis
(Wiley-V C H Verlag Gmbh, 2024) Yırtıcı, Ümit
The inhibitory potential of 17 flavonoids on lactate dehydrogenase A (LDHA), a key enzyme in the downstream process of aerobic glycolysis in cancer cells, is investigated. Fisetin exhibited excellent inhibitory activity (IC50 = 0.066 mu M). Quercetin 3-beta-D-glucoside, quercetin 3-galactoside, luteolin, neoeriocitrin, and luteolin 7-O-beta-D-glucoside showed good inhibitory activity (IC50 = 1.397-15.730 mu M). Biochanin A, baicalein, quercetin, scutellarein-7-glucuronide, diosmetin, baicalein 7-O-beta-D-glucuronide, and apigenin 7-apioglucoside demonstrated moderate inhibitory activity (IC50 = 33.007-86.643 mu M). Eriodictyol, quercetin 7-O-beta-D-glucoside, apigenin 7-O-beta-D-glucoside, and epicatechin were inactive. The Lineweaver-Burk plot showed that fisetin competitively inhibits NADH binding (Ki = 0.024 mu M). Ki values for other compounds were calculated using the Cheng-Prusoff equation (Ki = 0.2799-2.1661 mu M). The study revealed that the inhibitory effect of flavonoids varies with the number and position of OH groups and bound sugars. Molecular docking analyses indicated that flavonoids exhibited strong interactions with the NADH binding site of LDHA through hydrophobic interactions and hydrogen bonds. Molecular dynamic simulations tested the stability of the fisetin-LDHA complex over 100 ns and showed fisetin's high binding affinity to LDHA, maintaining strong hydrogen bonds. The binding energy of fisetin with LDHA was -33.928 kcal/mol, indicating its effectiveness as an LDHA inhibitor. Consequently, flavonoids identified as strong inhibitors could be potential cancer treatment sources through LDHA inhibition. Fisetin, quercetin 3-beta-D-glucoside, quercetin 3-galactoside, and luteolin are shown to exhibit powerful inhibition effects, with IC50 values between 0.066 and 1.792 mu M. The details of possible interactions with the active site of lactate dehydrogenase A were analyzed by molecular docking and molecular dynamics simulation. image
Natural flavonoids as promising 6-phosphogluconate dehydrogenase inhibitor candidates: In silico and in vitro assessments
(Wiley-V C H Verlag Gmbh, 2024) Adem, Şevki; Yırtıcı, Ümit; Aydın, Mesut; Rawat, Ravi; Eyüpoğlu, Volkan
The primary strategy in the fight against cancer is to screen compounds that may be effective on different types of cancer. Compounds from plants seem to be a good source. The present study investigated the inhibitory effects of some flavonoids on the 6-phosphogluconate dehydrogenase (6-PGD) enzyme. We determined that quercetin, myricetin, fisetin, morin, apigenin, and baicalein exhibited powerful inhibition effects with IC50 values between 4.08 and 21.26 mu M, while luteolin, kaempferol, apiin, galangin, and baicalin showed moderate effects with IC50 values between 54.15 and 138.91 mu M. Quercetin competitively inhibited the binding of NADP and 6-phosphogluconate to the 6-PGD enzyme with Ki values of 0.527 +/- 0.251 and 0.374 +/- 0.138 mu M, respectively. We calculated Ki values using the Cheng-Prusoff equation as between 0.44 and 14.88 mu M. The possible interaction details of polyphenols with the active site of 6-PGD were analyzed with docking software. In silico and in vitro studies indicated that the -OH groups on the A and C ring of flavonoids bind to the enzyme's active site via hydrogen bonding, while the -OH groups on the C ring contributed significantly to the increase in the inhibitory potentials of the molecules. Molecular dynamic simulations tested the stability of the 6-PGD-quercetin complex during 100 ns. These phytochemicals were suitable for drug use when optimized with absorption, distribution, metabolism, excretion, and toxicity (ADMET) criteria. The effects of the studied compounds on cancer cell lines of potential targets were demonstrated by network analysis. In conclusion, this study suggests that flavonoids found to be potent inhibitors could serve as leading candidates to treat many cancers via 6-PGD inhibition. Quercetin, myricetin, fisetin, morin, apigenin, and baicalein are shown to exhibit powerful inhibition effects with IC50 values between 4.08 and 21.26 mu M. The interaction details with the active site of 6-phosphogluconate dehydrogenase were analyzed by molecular docking and molecular dynamics simulation software. These phytochemicals were generally suitable for drug use when optimized according to absorption, distribution, metabolism, excretion, and toxicity (ADMET) criteria.image
Carboxylate- and Sulfonate-Containing Quinazolin-4(3H)-one Rings: Synthesis, Characterization, and Carbonic Anhydrase I-II and Acetylcholinesterase Inhibition Properties
(Wiley-V C H Verlag Gmbh, 2023) Tokalı, Feyzi Sinan; Alim, Zuhal; Yırtıcı, Ümit
Quinazolines are a group of bioactive heterocyclic compounds with a wide range of biological activities and have gained an important place in the design of active drugs with various targets due to their pharmacological properties. Carbonic anhydrase (CA) and acetylcholinesterase (AChE) inhibitors are very important pharmacologically. In this study, inhibition effects of newly synthesized quinazolin-4(3H)-one derivatives on human erythrocyte CA-I (hCA-I) and CA-II (hCA-II) isoenzyme and AChE activity were investigated. The structures of the novel compounds were characterized by fourier-transform infrared (FTIR), nuclear magnetic resonance (NMR), and high-resolution mass spectroscopy (HRMS). All molecules showed strong inhibitory effect in all three enzymes. 4-[(4-Oxo-2-(phenoxymethyl)quinazolin-3(4H)-ylimino)methyl]phenyl furan-2-carboxylate for hCA-I (IC50: 205 nM), 4-[(4-oxo-2-(phenoxymethyl)quinazolin-3(4H)-ylimino)methyl]phenyl isobutyrate for hCA-II (IC50: 209 nM), and 4-[(4-oxo-2-(phenoxymethyl)quinazolin-3(4H)-ylimino)methyl]phenyl propionate for AChE (IC50: 14.2 nM) were the molecules that showed the strongest inhibitory effect. Molecular docking studies were carried out to elucidate the possible interaction mechanism of the molecules in the active site of the enzymes. The affinity scores of the most active compounds for hCA-I, hCA-II, and AChE were determined as -134.765, -147.423, and -175.354 MolDock Score, respectively.
Blood and Stool Arsenic Levels Are Decisive for Diagnosing Children's Functional Gastrointestinal Disease (FGD)
(Springernature, 2022) Bilici, Namık; Doğan, Erkan; Sevinç, Eylem; Sevinç, Nergiz; Akıncı, Gülsema; Musmul, Ahmet; Cengiz, Mustafa
Pediatric gastroenteritis is a potentially fatal disease that accounts for 10% of childhood deaths. The main risk is environmental factors and nutrition. Arsenic (As) is commonly found in the earth's crust. As is an essential element that can form many organic compounds. In children, it causes diarrhea, gums, tongue lesions, diabetes, conjunctivitis, ocular opacity, and impaired immune response. It also causes low growth, mental retardation, and neurological problems. It is also known as the cause of many cancers that originate at an early age. Regionally, there is an iron and steel industry for almost a century. According to the Rome IV criteria, the blood and stools of 50 children aged 6-18 years, male and female, living in our province with functional gastrointestinal disease (FGD), were screened for As, and compared with the Healthy group (control) of 30 children. The results were evaluated with the Mann-Whitney Rank Sum Test. When blood and stool As values in males were compared with control samples, a high level of significance (p = 0.001) was found between both blood and stool As values in sick males and the control group (p < 0.005). In females, blood and stool As median values were also highly significant when compared with the control group (p = 0.001). According to these data, when the sick children (children with male and female gender) are compared with the healthy ones, the difference is highly significant (p < 0.005). High blood As levels in children indicate environmental pollution. It can be said that blood As levels are high as a result of food, water, and inhaler exposure. The presence of a high level of significant difference in stool means that the amount of As is high in the foods consumed daily. High levels of As are in blood and stools; It was evaluated that FGD could be the cause of nausea, diarrhea, vomiting, and colic. The increase in blood and stool As values due to environmental pollution is an important reason for FGD. For diseases of uncertain cause (such as FGD) resulting from chronic As exposure, blood and especially stool As values are more significant than urinary As levels. In conclusion, As a diagnostic criterion, it was concluded that blood and stool As values are an important marker in children with functional abdominal pain with other metals.
A new species of Zodarion (Araneae: Zodariidae) from Turkey
(Taylor & Francis Ltd, 2021) Coşar, İlhan
A new species of spider similar to Zodarion van Bosmans, 2009 is described based on material of both sexes from Kahramanmaras province, Turkey. Characteristics features and photographs of new species and Zodarion van Bosmans, 2009 are presented.
Technology in nursing education: Augmented reality
(Pamukkale Univ, 2021) Pınar Martlı, Emine; Ünlüsoy Dinçer, Nigar
The augmented reality technology, in which virtual images are combined with real world objects in real time, has begun to take part in the education of Generation Z, 21st century youth. Today, augmented reality applications that contribute to the learning process are also utilized in the field of nursing education. When database research is conducted with the combinations of nursing education and augmented reality keywords, it is seen that various augmented reality applications are used for improving English proficiency, anatomy knowledge and some of the nursing practices of nursing students. Research results show that students' learning experiences are improved with the use of augmented reality method thus learning becomes interesting and useful. This review includes the definition of augmented reality technology which increases the reality of environment and improves learning by embodying abstract concepts, its importance and place in nursing education, and the results of various studies on this subject.
Contributions to the genus Zodarion Walckenaer, 1826 in Turkey, with the description of a new species (Araneae: Zodariidae)
(Tubitak Scientific & Technological Research Council Turkey, 2021) Coşar, İlhan; Danışman, Tarık; Yağmur, Ersen Aydın
In this paper, two species are newly recognized in Turkey. One is new to science, described as Zodarion kunti sp. n. (male, female) from southeast Anatolian region. The other, Zodarion christae Bosmans, 2009 is found for the first time in Denizli, Antalya, and Mugla provinces.
The protective effects of selenium and boron on cyclophosphamide-induced hepatic oxidative stress, inflammation, and apoptosis in rats
(Elsevier Ltd, 2024) Cengiz, Mustafa; Gür, Bahri; Gür, Fatma; Şahintürk, Varol; Bayrakdar, Alpaslan; Şahin, Ilknur Kulcanay; Başkoy, Sıla Appak
Cyclophosphamide (CP) is an alkylating anticancer drug with broad clinical application that is highly effective in the treatment of cancer and non-malignant diseases. However, the main limiting effect of CP is multi-organ toxicity due to damage to normal tissues. The aim of this study is to compare the hepatoprotective potential of selenium (Se) and boron (B) in CP-induced liver injury in experimental rats. The rats were randomly divided into six equal groups: Control (saline), 200 mg/kg CP (administered once on the fourth day of the experiment), 1.5 mg/kg Se (administered once/time daily for 6 days), 20 mg/kg B (administered once/time daily for 6 days), Se + CP and B + CP administered intraperitoneally (i.p.). Administration of CP leads to an increase in the levels of apoptotic markers (Bax, caspase-3), the apoptotic signaling pathway (Nrf2), oxidative stress indicators (TOS, OSI), lipid peroxidation markers (MPO, MDA), inflammation levels (NF-kB, TNF-?, IL-1?, IL -6), liver function markers (ALT, AST, ALP), while apoptosis markers (Bcl-2), apoptosis pathway (Keap-1), oxidative stress indicator (TAS), inflammation (IL -10) and intracellular antioxidant defense system (SOD, CAT, GPx and GSH) decreased. In addition, degeneration of hepatocytes and congestion in the central veins were observed. In contrast, in the groups administered Se and B with CP, the changes that occurred were reversed. However, it was found that Se protects the liver slightly better against CP damage than B. The protective effect of Se and B against the toxic effects of CP on the antioxidant markers SOD, CAT and GPx1 was also investigated in silico. The in silico results were consistent with the in vivo results for SOD and CAT, but not for GPx1. © 2024
Myelosuppression and Oxidative Stress Induced by Cyclophosphamide in Rats: The Protective Role of Selenium
(Adiyaman University, 2019) Ayhancı, Adnan; Heybeli, Nilhan; Kulcanay Şahin, İlknur; Cengiz, Mustafa
Aim of this study is to detect the protective role of selenium (Se) against bone marrow and blood toxicity in CP-induced hematoxicity model. We categorized the rats into 6 groups of 7 animals in each group (control; 150 mg/kg CP; 0.5 mg/kg Se; 1 mg/kg Se; 150+0.5 mg/kg CP+Se; 150+1 mg/kg CP+Se). Se injections started 5 days before the CP injections and carried on until the end of the experiment (6th day) for the groups to which CP was injected together with Se. CP was applied as a single dose before anesthesia. For that reason, on the 7th day, blood was taken with cardiac puncture and bone marrow was taken by flushing the femur. Peripheral blood cells and bone marrow nucleated cells were counted on a cell counter. Intraperitoneal CP injection was found to reduce the number of leukocytes by 317%, thrombocyte by 36% and bone marrow nucleated cells by 481% compared to the control group. In the groups where CP was given after 0.5 and 1 mg/kg Se, numbers of leukocyte, thrombocyte and bone marrow nucleated cells were considerably improved compared to the group to which CP was given only (p<0.001). Results show that 1 mg/kg Se has a better protection than 0.5 mg/kg against CP associated hematoxicity and myelosuppression. Our results also imply that the doses of Se could be adjusted according to enhance in CP dose so as to gain a stronger protective effect. We believe there is a need of further studies in which different doses of Se will be used against CP induced hematoxicity. Se can provide protection against CP-induced myelosupression and lipid peroxidation. © 2019, Adiyaman University. All rights reserved.
Mediterranean-DASH Diet Intervention for Neurodegenerative Delay Diet and Psychological Problems in Maintenance Hemodialysis Patients by the Structural Equation Modeling Analysis of Malnutrition and Inflammation Markers Approach
(W.B. Saunders, 2024) Açik, Murat; Bayindir Gümüş, Aylin; Ekici, Ayşe; Çağiran Yilmaz, Feray; Küçüksu, Mehmet
Objective: Although it is known that diet quality affects psychological problems in hemodialysis (HD) patients, there is need to explain the role of modifiable risk factors in this relationship. Therefore, the aim of this study was (1) to investigate the relationship between diet quality and modifiable risk factors to depression and anxiety in end-stage renal disease patients receiving maintenance HD; (2) to explore the mediating roles of modifiable factors in the relationship with diet quality to depression and anxiety. Methods: This cross-sectional study included 216 patients with end-stage renal disease receiving maintenance HD treatment. We assessed participants' dietary records for Mediterranean-DASH Diet Intervention for Neurodegenerative Delay (MIND) quality, Global Leadership Initiative on Malnutrition criteria for malnutrition and Hospital Anxiety and Depression Scale for psychological problems. Furthermore, biochemical findings and anthropometric measurements were performed to evaluate nutritional markers, metabolic risk factors and inflammation. We applied hierarchical regression analysis to estimate modifiable risk factors for depression and anxiety and structural-equation-modeling analysis to determine the mediating role of modifiable risk factors between diet quality and psychological problems. Results: Depression symptoms were observed in 59.2% (n = 128) of the participants, whilst the rate was 35.1% (n = 76) for anxiety. Depression and anxiety were found to be negatively correlated with MIND levels after covariate adjusting model, and the rates of explanation were found to be 16.2% and 12.2%, respectively. C-reactive protein (CRP), albumin levels and the presence of malnutrition were shown to be significant predictors of depression (?F = 14.761 and ?R2: 0.071 for covariate-adjusted model). Albumin levels, CRP, HD duration, and malnutrition were found to be independent predictors of anxiety (?F = 16.174 and ?R2: 0.077 for covariate-adjusted model). It was found that CRP and malnutrition partially mediated the association of MIND score with depression, and CRP mediated the association with anxiety. Conclusion: It was concluded that adherence to the MIND diet is associated with a better nutritional profile and reduced inflammation, which in turn may be linked to fewer psychological problems.These further studies are needed to validate and expand upon our findings. © 2024 National Kidney Foundation, Inc.
Centaurea mersinensis phytochemical composition and multi-dimensional bioactivity properties supported by molecular modeling
(Taylor and Francis Ltd., 2024) Yırtıcı, Ümit; Ergene, Aysun; Adem, Şevki; Atalar, Mehmet Nuri; Eyüpoğlu, Volkan; Rawat, Ravi; Arat, Esra
Various studies conducted on Centaurea species indicate that the relevant plant is good source of bioactive phytochemicals. In this study, in vitro studies were used to determine bioactivity properties of methanol extract of Centaurea mersinensis - endemic species in Turkey - on extensive basis. Furthermore, the interaction of target molecules, identified for breast cancer and phytochemicals in the extract, was investigated via in silico analyses to support findings received in vitro. Scutellarin, quercimeritrin, chlorogenic acid and baicalin were primary phytochemicals in the extract. Methanol extract and scutellarin had higher cytotoxic effects against MCF-7 (IC50=22.17 µg/mL, and IC50=8.25 µM, respectively), compared to other breast cancer cell lines (MDA-MB-231, SKBR-3). The extract had strong antioxidant properties and inhibited target enzymes, especially ?-amylase (371.69 mg AKE/g extract). The results of molecular docking indicate that main compounds of extract show high-strength bonding to the c-Kit tyrosine among target molecules identified in breast cancer, compared to other target molecules (MMP-2, MMP-9, VEGFR2 kinase, Aurora-A kinase, HER2). The tyrosinase kinase (1T46)-Scutellarin complex showed considerable stability in 150 ns simulation as per MD findings, and it was coherent with optimal docking findings. Docking findings and HOMO-LUMO analysis results corresponds with in vitro experiments. Medicinal properties of phytochemicals, which was determined to be suitable for oral use along with ADMET, were found to be within normal limits except for their polarity properties. In conclusion, in vitro and in silico studies indicated that the relevant plant yields promising results regarding its potential to develop novel and effective medicational products. Communicated by Ramaswamy H. Sarma. © 2023 Informa UK Limited, trading as Taylor & Francis Group.
Blood and Stool Arsenic Levels Are Decisive for Diagnosing Children’s Functional Gastrointestinal Disease (FGD)
(Humana Press Inc., 2022) Bilici, Namik; Doğan, Erkan; Sevinç, Eylem; Sevinç, Nergiz; Akinci, Gülsema; Musmul, Ahmet; Cengiz, Mustafa
Pediatric gastroenteritis is a potentially fatal disease that accounts for 10% of childhood deaths. The main risk is environmental factors and nutrition. Arsenic (As) is commonly found in the earth’s crust. As is an essential element that can form many organic compounds. In children, it causes diarrhea, gums, tongue lesions, diabetes, conjunctivitis, ocular opacity, and impaired immune response. It also causes low growth, mental retardation, and neurological problems. It is also known as the cause of many cancers that originate at an early age. Regionally, there is an iron and steel industry for almost a century. According to the Rome IV criteria, the blood and stools of 50 children aged 6–18 years, male and female, living in our province with functional gastrointestinal disease (FGD), were screened for As, and compared with the Healthy group (control) of 30 children. The results were evaluated with the Mann–Whitney Rank Sum Test. When blood and stool As values in males were compared with control samples, a high level of significance (p = 0.001) was found between both blood and stool As values in sick males and the control group (p < 0.005). In females, blood and stool As median values were also highly significant when compared with the control group (p = 0.001). According to these data, when the sick children (children with male and female gender) are compared with the healthy ones, the difference is highly significant (p < 0.005). High blood As levels in children indicate environmental pollution. It can be said that blood As levels are high as a result of food, water, and inhaler exposure. The presence of a high level of significant difference in stool means that the amount of As is high in the foods consumed daily. High levels of As are in blood and stools; It was evaluated that FGD could be the cause of nausea, diarrhea, vomiting, and colic. The increase in blood and stool As values due to environmental pollution is an important reason for FGD. For diseases of uncertain cause (such as FGD) resulting from chronic As exposure, blood and especially stool As values are more significant than urinary As levels. In conclusion, As a diagnostic criterion, it was concluded that blood and stool As values are an important marker in children with functional abdominal pain with other metals. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Adaptation of the scale of effects of social media on eating behavior in Hungarian university students
(National Institute of Public Health NIH – National Research Institute, 2024) Bayındır-Gümüş, Aylin; Öztürk, Ebru; Soós, Mihály
Background. People live in a technological world, where social media is used very commonly. Social media has effects on eating behaviors, as in other aspects. For this reason, it is important to measure social media effect. Objective. This study aimed to adapt the Scale of Effects of Social Media on Eating Behaviour (SESMEB) that examines the effect of social media on eating behavior in Hungarian university students. Material and methods. The SESMEB was translated into the target language by taking various stages. The online questionnaire including general information, social media use, and the eighteen-item SESMEB was used to collect data. The scale was administered to the study group consisting of 213 Hungarian university students, and data from 203 of them were analyzed. Confirmatory factor analyses were performed to test construct validity, and the Cronbach alpha coefficient was calculated for the reliability of the scale in Hungarian. Results. Total correlation value was higher than 0.50 for all items of the scale. The fit indices were at an acceptable level or had a perfect fit. The t-values were significant at the level of 0.1 and ranged between 2.927 and 5.706. The Spearman–Brown coefficient was calculated at 0.894. The reliability coefficient of the scale was calculated to be 0.866. SESMEB scores were different according to spending time daily, sharing content, and using filters or Photoshop on social media (p<0.05). Conclusions. Higher than 0.80 Cronbach’s alpha coefficient and other results show that Hungarian SESMEB is a valid and reliable tool. Therefore, Hungarian SESMEB will be useful for further studies to determine the impact of social media on eating behaviors. © 2024 National Institute of Public Health NIH â€“ National Research Institute. All rights reserved.
Adaptation of “Food Chain Engagement Measurement” Scale for Adults into Turkish: A Validity and Reliability Study
(Sidas Medya A.S., 2024) Gümüş, Aylin Bayındır; Durmaz, Sevinç Eşer; Kılınç, Fatma Nişancı; Çakır, Biriz; Özenir, Çiler; Öztürk, Ebru
This study aimed to adapt to Turkish culture the "Food Chain Engagement Measurement Scale” (FCEMS), which determines the participation of individuals in the food chain and consists of the sub-dimensions of obtaining communication, waste reduction, planning, and preparation. It aimed to evaluate FCEMS scores according to their general and food purchasing-meal preparation characteristics. The scale was translated from English into the target language, Turkish, by experts, and after the necessary controls, it was translated back into the primary language. It was finalized after final checks and the pilot study. A questionnaire containing general information, information about purchasing and preparing food, and the 'Food Chain Engagement Measurement Scale' was applied to the participants, and data was collected by face-to-face interview technique. A total of 1127 adults, 712 women and 415 men, were included in the study. The mean FCEMS score was higher in women, those with higher education levels, married, living in urban areas, with chronic diseases, and on a diet (p<0.05). FCEMS score varied depending on the behaviors of purchasing and preparing food of participants (p<0.05). The factor loadings of the scale items adapted to Turkish ranged between 0.42 and 0.803, and the fit statistics were found to be high. The total Cronbach's alpha coefficient of the scale was calculated as 0.804. As a result of analyses, it was concluded that the scale is a valid and reliable measurement tool in Turkish and can be applied to adults. © 2024 Sidas Medya A.S.. All rights reserved.

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