Makale Koleksiyonu

Bu koleksiyon için kalıcı URI

https://hdl.handle.net/20.500.12587/18179

Listeleniyor 1 - 18 / 18

Blood and Stool Arsenic Levels Are Decisive for Diagnosing Children's Functional Gastrointestinal Disease (FGD)
(Springernature, 2022) Bilici, Namık; Doğan, Erkan; Sevinç, Eylem; Sevinç, Nergiz; Akıncı, Gülsema; Musmul, Ahmet; Cengiz, Mustafa
Pediatric gastroenteritis is a potentially fatal disease that accounts for 10% of childhood deaths. The main risk is environmental factors and nutrition. Arsenic (As) is commonly found in the earth's crust. As is an essential element that can form many organic compounds. In children, it causes diarrhea, gums, tongue lesions, diabetes, conjunctivitis, ocular opacity, and impaired immune response. It also causes low growth, mental retardation, and neurological problems. It is also known as the cause of many cancers that originate at an early age. Regionally, there is an iron and steel industry for almost a century. According to the Rome IV criteria, the blood and stools of 50 children aged 6-18 years, male and female, living in our province with functional gastrointestinal disease (FGD), were screened for As, and compared with the Healthy group (control) of 30 children. The results were evaluated with the Mann-Whitney Rank Sum Test. When blood and stool As values in males were compared with control samples, a high level of significance (p = 0.001) was found between both blood and stool As values in sick males and the control group (p < 0.005). In females, blood and stool As median values were also highly significant when compared with the control group (p = 0.001). According to these data, when the sick children (children with male and female gender) are compared with the healthy ones, the difference is highly significant (p < 0.005). High blood As levels in children indicate environmental pollution. It can be said that blood As levels are high as a result of food, water, and inhaler exposure. The presence of a high level of significant difference in stool means that the amount of As is high in the foods consumed daily. High levels of As are in blood and stools; It was evaluated that FGD could be the cause of nausea, diarrhea, vomiting, and colic. The increase in blood and stool As values due to environmental pollution is an important reason for FGD. For diseases of uncertain cause (such as FGD) resulting from chronic As exposure, blood and especially stool As values are more significant than urinary As levels. In conclusion, As a diagnostic criterion, it was concluded that blood and stool As values are an important marker in children with functional abdominal pain with other metals.
Technology in nursing education: Augmented reality
(Pamukkale Univ, 2021) Pınar Martlı, Emine; Ünlüsoy Dinçer, Nigar
The augmented reality technology, in which virtual images are combined with real world objects in real time, has begun to take part in the education of Generation Z, 21st century youth. Today, augmented reality applications that contribute to the learning process are also utilized in the field of nursing education. When database research is conducted with the combinations of nursing education and augmented reality keywords, it is seen that various augmented reality applications are used for improving English proficiency, anatomy knowledge and some of the nursing practices of nursing students. Research results show that students' learning experiences are improved with the use of augmented reality method thus learning becomes interesting and useful. This review includes the definition of augmented reality technology which increases the reality of environment and improves learning by embodying abstract concepts, its importance and place in nursing education, and the results of various studies on this subject.
Contributions to the genus Zodarion Walckenaer, 1826 in Turkey, with the description of a new species (Araneae: Zodariidae)
(Tubitak Scientific & Technological Research Council Turkey, 2021) Coşar, İlhan; Danışman, Tarık; Yağmur, Ersen Aydın
In this paper, two species are newly recognized in Turkey. One is new to science, described as Zodarion kunti sp. n. (male, female) from southeast Anatolian region. The other, Zodarion christae Bosmans, 2009 is found for the first time in Denizli, Antalya, and Mugla provinces.
Centaurea fenzlii Reichardt Özütünün Antioksidan Özellikleri ve Enzim İnhibisyon Etkisinin Belirlenmesi
(2019) Yırtıcı, Ümit
Bu çalışmada Centaurea fenzlii Reichardt bitkisinin antioksidan özellikleri ve çeşitli enzimler üzerine inhibe edici etkisi araştırıldı. Bu amaçla çiçeklenme döneminde toplanan Centaurea fenzlii Reichardt’ın toprak üstü kısımları farklı polariteye sahip çözücülerde maserasyon yöntemi kullanılarak çözüldü ve ekstreleri elde edildi. Çalışmalara metanol ekstresi ile devam edildi. Metanol ekstresinin toplam fenolik ve flavonoid içerikleri sırasıyla, 16,72 mg GAE/g ka ve 173,16 mg KAE/g ka olarak belirlendi. Antioksidan kapasiteleri demir indirgeyici gücü (FRAP) için 0,256 mmol TE/g ka, bakır indirgeyici gücü (CUPRAC) için 0,878 mmol TE/g ka, ABTS için 0,354 mmol TE/g ka ve DPPH için 0,661 mmol TE/g ka olarak saptandı. Ayrıca, ekstrelerinin kolinesteraz, ?-amilaz, ?-glukozidaz ve tirozinaz enzimlerine karşı inhibe edici etkileri de belirlendi. Enzim inhibisyon etkisi sırasıyla, ?-Glukozidaz için 0,331 mmol AKE/g ka, ?-Amilaz için 0,354 mmol AKE/g ka, AChE için 0,367 mmol GAE/g ka, BChE için 0,878 mmol GAE/g ka ve Tirozinaz için mmol 0,256 KE/g ka olarak bulundu.
A New Species Of The Genus Zodarion Walckenaer, 1826 (Araneae: Zodariidae) From Turkey
(AMER ENTOMOL SOC, 2020) Danisman, Tarik; Cosar, Ilhan
Anew species, Zodarion izmirense sp. nov. is described and illustrated based on material collected in the Izmir Province of Turkey. Differences between the new species and the closely related Zodarion tireboluensis Danisman and Rubio, 2017 are discussed.
Protective Effects of Boron on Cyclophosphamide-Induced Bladder Damage and Oxidative Stress in Rats
(HUMANA PRESS INC, 2020) Ayhancı, Adnan; Tanriverdi, Döndü Tuğçe; Şahintürk, Varol; Cengiz, Mustafa; Appak-Baskoy, Sıla; Şahin, İlknur Kulcanay
This study aims to investigate protective effects of boron against cyclophosphamide-induced bladder toxicity that produces oxidative stress and leads to apoptosis of the cells. In total, 24 rats were divided into 4 equal groups. The control group received saline. The 2nd experimental group received 200 mg kg of cyclophosphamide i.p. on the 4th day while the 3rd group was given only boron (200 mg kg, i.p.) for 6 days. In the 4th group, boron was given for 6 days and cyclophosphamide (200 mg kg, i.p.) was administrated on the 4th day. Twenty-four hours after the last boron or cyclophosphamide administration, rats were sacrificed under anesthesia. Bladder tissues of rats were taken for histological and immunohistochemical (apoptotic markers such as caspase-3, bcl-2, and bax) and blood was taken for the biochemical (serum total thiol, serum natural thiol, serum thiol-disulfide) analysis. Transient epithelial thinning, edema, marked inflammatory reaction, and bleeding were observed in bladders of the group that received cyclophosphamide. Also, the activity of bax and caspase-3-positive cells increased while the number of bcl-2-positive cells decreased. In the same group, serum natural thiol and total thiol levels decreased while serum disulfide levels increased, which indicates oxidative stress. On the other hand, in the boron+cyclophosphamide group pretreatment with boron protected, the bladder tissue and the number of bcl-2-positive cells increased, and bax and caspase-3-positive cells decreased, showing antiapoptotic effects of boron against cyclophosphamide-induced toxicity. In parallel with the findings of this group, native thiol and total thiol levels increased and serum disulfide levels decreased pointing out to a decreased oxidative stress. Our results indicate that boron pretreatment significantly protects rat bladder against cyclophosphamide-induced bladder damage due to its antiapoptotic and antioxidant properties.
Cyclophosphamide induced oxidative stress, lipid per oxidation, apoptosis and histopathological changes in rats: Protective role of boron
(ELSEVIER GMBH, 2020) Cengiz, Mustafa; Sahinturk, Varol; Yildiz, Songul Cetik; Sahin, Ilknur Kulcanay; Bilici, Namik; Yaman, Suzan Onur; Altuner, Yilmaz
Background: Cyclophosphamide (CP) is an alkylating chemotherapeutic drug used in the treatment of many types of cancer. However, as with other chemotherapeutic drugs, the use of CP is limited by the damage to healthy tissues such as testes, bladder and liver as well as cancerous tissue. Boron (B) is a trace element with many biological properties such as antioxidant, anti-apoptotic and anti-lipid per oxidation. Methods: This current study aims to determine protective effects of B on CP induced testicular toxicity. The rats were divided into 4 groups (control, CP, B and B plus CP groups). The testes of experimental animals were taken for histological, apoptotic markers and biochemical analysis. Results: The damage to some seminifer tubules, loss of typical appearance, thinning of seminifer epithelium and relative enlargement of the tubule lumen were watched in testis of the group that administrated CP. Moreover, Bcl-2, TAC and GSH levels decreased while TOC, OSI, MDA, Bax and Caspase-3 levels increased. On the other hand, pretreatment limited to B in the B plus CP group, testicular tissue improved. In addition, Bcl-2, GSH, TAC levels increased, Bax, MDA, TOC, OSI and caspase-3 levels decreased. Conclusion: B significantly reduced testicular lipid per-oxidation and strengthened antioxidant defenses. Our results showed that pre-treatment B can protect rat testis against CP-induced testicular damage owing to its anti-lipid per oxidation, anti-oxidant and anti-apoptotic properties.
Hepato-preventive and anti-apoptotic role of boric acid against liver injury induced by cyclophosphamide
(Elsevier Gmbh, 2019) Cengiz, Mustafa; Yildiz, Songul Cetik; Demir, Cemil; Sahin, Ilknur Kulcanay; Teksoy, Ozgun; Ayhanci, Adnan
This study aims to examine cyclophosphamide (CP) exsposure associated toxicity on rat livers and the likely defensive effects of boric acid (BA). The rats used in this study were divided into four groups: control group, CP group, BA group, and BA + CP group. The present study was carried out using routine histological H&E stain, immunohistochemical stain caspase-3 as apoptotic marker, serum biochemical analysis for liver function markers (alanine transaminase (ALT), aspartate transaminase (AST) and alkalen phosphatase (ALP)), oxidative stress markers (total oxidant status (TOS), oxidative stress index (OSI) and total antioxidant capacity marker (TAC)). In the CP group, the levels of ALT, AST, ALP, TOS, OSI and caspase-3 increased whereas TAC levels decreased compared with the control group. In the BA + CP group, the levels of ALT, AST, ALP, TOS, OSI and caspase-3 decreased whereas TAC levels increased compared with the CP group. The histopathological evaluation of light microscope images and immunohistochemical caspase-3 activity in the BA + CP group were found to be decrease compared with those in the CP group. In conclusion, BA was successful in defending the liver against apoptosis and histopathological changes that are attributable to CP.
The Investigation of the Cox-2 Selective Inhibitor Parecoxib Effects in Spinal Cord Injury in Rat
(Taylor & Francis Inc, 2019) Yuksel, Ulas; Bakar, Bulent; Dincel, Gungor Cagdas; Yildiran, Fatma Azize Budak; Ogden, Mustafa; Kisa, Ucler
Aim: Today, spinal cord injury (SCI) can be rehabilitated but cannot be treated adequately. This experimental study was conducted to investigate possible beneficial effects of methylprednisolone and parecoxib in treatment of SCI. Materials and methods: Forty-eight male Wistar albino rats were assigned into CONTROL, acute (MP-A, PX-A, and PXMP-A), and subacute (MP-S, PX-S, and PXMP-S) stage groups. Then, to induce SCI, a temporary aneurysm clip was applied to the spinal cord following T7-8 laminectomy, except in the CONTROL group. Four hours later parecoxib, methylprednisolone, or their combination was administered to rats intraperitoneally except CONTROL, SHAM-A, and SHAM-S groups. Rats in the acute stage group were sacrificed 72 h later, and whereas rats in the subacute stage were sacrificed 7 days later for histopathological and biochemical investigation and for gene-expression analyses. Results: Parecoxib and methylprednisolone and their combination could not improve histopathological grades in any stage. They also could not decrease malondialdehyde or caspase-3, myeloperoxidase levels in any stage. Parecoxib and methylprednisolone could decrease the TNF-alpha gene expression in subacute stage. Methylprednisolone could increase TGF-1 beta gene-expression level in acute stage. Conclusion: Neither of the experimental drugs, either alone or in combination, did not show any beneficial effects in SCI model in rats.
Elevated Tear Human Neutrophil Peptides 1-3, Human Beta Defensin-2 Levels and Conjunctival Cathelicidin LL-37 Gene Expression in Ocular Rosacea
(Taylor & Francis Inc, 2019) Gokcinar, Nesrin Buyuktortop; Karabulut, Ayse Anil; Onaran, Zafer; Yumusak, Erhan; Yildiran, Fatma Azize Budak
Purpose: To investigate the role of innate immunity in ocular rosacea. Methods: Thirty-two patients with ocular rosacea patients (group-1) and 28 healthy volunteers (group-2) who served as controls were enrolled in the study. Tear function parameters were assessed, conjunctival impression cytology was performed and tear samples were collected. Human-neutrophil-peptides (HNP) 1?3 and human-beta-defensin-2 (hBD-2) levels were measured in tears by using ELISA tests. Cathelicidin leucin-leucin-37 (LL-37), hBD-2, human-beta-defensin-9 (hBD-9) gene expression levels were measured in the conjunctival impression cytology samples using real-time polymerase chain reaction. Results: Tear HNP1-3 (p?=?0.024), hBD-2 (p?
Cytotoxic and apoptotic effects of endemic Centaurea fenzlii Reichardt on the MCF-7 breast cancer cell line
(Tubitak Scientific & Technical Research Council Turkey, 2017) Yirtici, Umit; Goger, Fatih; Sarimahmut, Mehmet; Ergene, Aysun
The main purpose of this study was to analyze the cytotoxic activity of an extract obtained from Centaurea fenzlii Reichardt, and the fractions eluted from this extract, in breast cancer cells. After isolation and structural analysis of the fractions were conducted, a meaningful cytotoxic effect was indicated. The goal of the analysis was to reveal the mechanism by which this effect occurs through researching the apoptotic side of these fractions and determining the amount of several proteins that are the products of the genes. Test substances were applied to breast cancer cells and the inhibitory concentration value 50 (IC50) that caused a cytotoxic effect was determined using MTT and ATP assays. The Centaurea fenzlii Reichardt dichloromethane extracts-ethyl acetate fractions (CFDCM-EAF) exhibited a stronger growth-inhibitory effect on MCF-7 cells (45.771 mu g/mL). The apoptotic effect was studied using double staining and flow cytometry. The death rate in the cells treated with the CFDCM-EAF IC50 dose was approximately 90%: 9.2% living cells, 22.8% necrotic cells, 62.3% late apoptotic cells, and 5.8% early apoptotic cells. Structural analysis of the CFDCM-EAF, which indicated significant cytotoxic effects, was performed using chromatographic methods. Hispidulin was the major component of the CFDCM-EAF by LC-APCI-MS/MS analysis.
Effects of DMSO on a rabbit ear hypertrophic scar model: A controlled randomized experimental study
(Elsevier Sci Ltd, 2017) Sari, Elif; Bakar, Bulent; Dincel, Gungor Cagdas; Yildiran, Fatma Azize Budak
Dimethyl sulfoxide (DMSO) is an anti-inflammatory, antibacterial, analgesic drug widely used to treat several diseases as reported in the literature. It has a detractive effect on collagen deposition in the abnormal tissue. This study aimed to investigate the possible therapeutic effects of DMSO on hypertrophic scar formation in rabbits. Twenty-four New Zealand male albino rabbits were randomly divided into four groups: control, sham, DMSO, and TRA (triamcinolone acetonide). Except the control group, punch biopsy defects were created on each animal's right ear. Following the hypertrophic scar formation on day 28, intralesional DMSO and triamcinolone acetonide were administered once a week for 4 weeks into these scars of the DMSO and TRA groups, respectively. No therapeutic agent was administered to the control and sham groups. One week after the last injection, ear samples were collected for histopathological, immunohistochemical, and real-time polymerase chain reaction gene expression analyses. Histopathological examination revealed that the epithelium in the DMSO group was thicker than that in the control and TRA groups, but thinner than that in the sham group. Connective tissue thickness and vascularity level of the sham group were higher than those of the control, DMSO, and TRA groups. The collagen type I immunoreactivity level of the sham and TRA groups was higher than those of the control and DMSO groups. The collagen type III immunoreactivity level was higher in the sham group than in all other groups. Collagen type I/type III immunoreactivity ratios were lower in the DMSO group. The alignment of collagen fibers was normal in the DMSO group, but was irregular in the sham and TRA groups. The collagen type I gene expression levels of the DMSO and TRA groups were lower than that of the sham group. Collagen type III and IFN-gamma mRNA expression levels were almost similar among the groups. TGF-1 beta mRNA expression levels were higher in the DMSO and TRA groups than in the control and sham groups. On the basis of the results, it can be concluded that intralesional administration of DMSO decreases hypertrophic scar formation easily and safely. (C) 2017 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.
Cardioprotective Effect of Selenium Against Cyclophosphamide-Induced Cardiotoxicity in Rats
(Humana Press Inc, 2017) Gunes, Sibel; Sahinturk, Varol; Karasati, Pinar; Sahin, Ilknur Kulcanay; Ayhanci, Adnan
The objective of this study is to evaluate the possible protective effects of selenium (Se) against cyclophosphamide (CP)-induced acute cardiotoxicity in rats. A total of 42 male Spraque-Dawley rats were divided into six groups (n = 7). Rats in the first group were served as control. Rats in the second group received CP (150 mg/kg) at the sixth day of experiment. Animals in the third and fourth groups were treated with only 0.5 and 1 mg/kg Se respectively for six consecutive days. Rats in the fifth and sixth groups received respective Se doses (0.5 or 1 mg/kg) for 6 days and then a single dose of CP administered on the sixth day. On day 7, the animals were sacrificed; blood samples were collected to measure malondialdehyde (MDA), glutathione (GSH), lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), and ischemia-modified albumin (IMA) levels. Heart tissues were processed routinely and tissue sections were stained with H + E for light microscopic examination. In the CP-treated rats MDA, LDH, CK-MB, and IMA serum levels increased, while GSH levels decreased. Microscopic evaluation showed that tissue damage was conspicuously lower in CP plus Se groups. Moreover, 1 mg/kg Se was more protective than 0.5 mg/kg Se as indicated by histopathological and biochemical values. In conclusion, Se is suggested to be a potential candidate to ameliorate CP-induced cardiotoxicity which may be related to its antioxidant activity.
The Presence of Metals and Antibiotics Resistant Bacteria in Arable Manure Soils
(Polish Acad Sciences Inst Physics, 2017) Erdem, B.; Dayangac, A.; Sahin, I. K.
Heavy metals and antibiotics resistance are intense public interest owing to their pollution in the environment and potential deleterious effects on human health. A total of 15 isolates of Bacillus subtilis, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli (50 manure garden soils) were tested for their resistance against certain heavy metals and antibiotics. Minimum inhibitory concentrations of Pb2+, Co2+, Cu2+, and Zn2+ for each isolate were also determined. B. subtilis, B. cereus, P. aeruginosa and E. coli exhibited high tolerance to minimum inhibitory concentrations of 3,200 mu g/ml for Pb2+, 400 mu g/ml for Cu2+ and Co2+, and 100 mu g/ml for Zn2+ in agar plates. All the isolates were highly resistant to lead and they showed 100% growth in 3,200 mu g/ml concentration. It was observed that all isolates were resistant to a wide range of antibiotics namely clindamycin, ceftazidime, aztreonam and vancomycin.
Four new records for the spider fauna of Turkey (Araneae: Salticidae)
(Tubitak Scientific & Technical Research Council Turkey, 2015) Cosar, Ilhan
Four Salticidae spider species, Euophrys sulphurea (L. Koch, 1867), Neon levis (Simon, 1871), Pellenes brevis (Simon, 1868), and Sibianor aurocinctus (Ohlert, 1865), are recorded for the first time from Turkey. Their morphology is briefly described and illustrated.
Composition of the Essential Oil of Some Centaurea L.
(Asian Journal Of Chemistry, 2014) Akkurt, A.; Celik, S.
Composition of the water-distilled oil of Centaurea kurdica, C. antochia var. antochia and Centaurea albonitens in Turkey, were analyzed by GC and GC-MS. In essential oil of Centaurea kurdica, caryophyllene 14.4 %, a - humulene 4.62 %; germacrene 11.95 %; beta-farnesene 9.78 %; spathulenol 7.73 %; glubulol 5.58 %; beta-eudesmol 4.04 %; in essential oil of C. antiochia var. antiochia caryophyllene 18.23 %; germacrene 27.37 %; spathulenol 29.86 %; hexadecanoic acid 7.21 % and in essential oil of C. albonitens gamma-elemene 4.45 %; caryophyllene 7.75 %; germacrene 9.23 %; spathulenol 7.97 %; caryophyllene oxide 16.45 %; cembrene 6.25 %; phytol 4.75 %; beta-selinenol 4.57 % were the main constituents.
Effectiveness of the biophysical barriers on the peridural fibrosis of a postlaminectomy rat model: An experimental research
(Elsevier Sci Ltd, 2011) Kasimcan, Mustafa Omur; Bakar, Bulent; Aktas, Savas; Alhan, Aslihan; Yilmaz, Mustafa
Purpose: Long term results after surgical treatment of disc herniation have shown that epidural and/or peridural fibrosis formed during the healing process after surgical intervention. We conducted this experimental study to evaluation of the effectiveness of the bioresorbable barriers (ADCON-L and Seprafilm (R) Adhesion Barrier) on formation of the peridural fibrosis in rat model performed laminectomy. Methods: Thirty-two male Wistar albino rats 250-350 g body weight were distributed into three groups (CONTROL, AL group received ADCON-L; SAB group received Seprafilm (R) Adhesion Barrier). A dorsal laminectomy at L3, L4, L5 was performed, and then except those of the CONTROL group, the experimental material was left on the dura mater. Six weeks later spinal column of all rats was totally removed between the T10 and L5 levels, and peridural fibrosis, and dural adhesions were evaluated histologically and graded. The results were compared statistically by using the chi-square (chi(2)) test. Also three random regions were examined, and the fibroblast cells were counted. The fibroblast count results were statistically analysed by using the One-Way ANOVA test. Results: The variation of histopathological grades was statistically significant regarding the comparison of the all groups obtained from the chi(2) test (chi(2) = 16.40; p = 0.003). However, the variation in the mean values of the fibroblast count result was not statistically significant obtained from the One-Way ANOVA test (F = 2.114; p > 0.05). Conclusion: Our study results suggest that Seprafilm (R) Adhesion Barrier and ADCON-L can be effective in reducing the prevalence of the postoperative peridural adhesions in rat laminectomy model. On the other hand, the fibroblast densities of the experimental groups were not different between groups. So, we could say that these materials can act as a foreign body in long term period in rat. (C) 2010 Elsevier Ltd. All rights reserved.
Molecular analysis of three local silkworm breeds (Alaca, Bursa Beyazi and Hatay Sarisi) by RAPD-PCR and SDS-PAGE methods
(Veteriner Fakultesi Dergisi, 2010) Eroğlu Akkir D.; Budak Yildiran F.A.; Çakir S.
In this study, PCR-based RAPD method was used to determine the genetic variation in three Turkish local silkworm breeds (Alaca, Bursa Beyazı and Hatay Sarısı) and SDS-PAGE method was used to analyse the sericin proteins of their cocoons. Three local breeds were analysed by randomly chosen 40 primers and 68 total RAPD bands observed with 7 of them. Percentages of polymorphic loci were determined higher in Hatay Sarısı (55.88), lower in Bursa Beyazı and Alaca (44.12). Nei's genetic distance was determined 0.0637 between Bursa Beyazı and Alaca, 0.1012 between Bursa Beyazı and Hatay Sarısı, 0.0793 between Alaca and Hatay Sarısı. In SDS-PAGE sericin proteins of three breeds was yielded a single band of 200 kDa.

Güncel Gönderiler