Natural flavonoids as promising lactate dehydrogenase A inhibitors: Comprehensive in vitro and in silico analysis
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Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The inhibitory potential of 17 flavonoids on lactate dehydrogenase A (LDHA), a key enzyme in the downstream process of aerobic glycolysis in cancer cells, is investigated. Fisetin exhibited excellent inhibitory activity (IC50 = 0.066 mu M). Quercetin 3-beta-D-glucoside, quercetin 3-galactoside, luteolin, neoeriocitrin, and luteolin 7-O-beta-D-glucoside showed good inhibitory activity (IC50 = 1.397-15.730 mu M). Biochanin A, baicalein, quercetin, scutellarein-7-glucuronide, diosmetin, baicalein 7-O-beta-D-glucuronide, and apigenin 7-apioglucoside demonstrated moderate inhibitory activity (IC50 = 33.007-86.643 mu M). Eriodictyol, quercetin 7-O-beta-D-glucoside, apigenin 7-O-beta-D-glucoside, and epicatechin were inactive. The Lineweaver-Burk plot showed that fisetin competitively inhibits NADH binding (Ki = 0.024 mu M). Ki values for other compounds were calculated using the Cheng-Prusoff equation (Ki = 0.2799-2.1661 mu M). The study revealed that the inhibitory effect of flavonoids varies with the number and position of OH groups and bound sugars. Molecular docking analyses indicated that flavonoids exhibited strong interactions with the NADH binding site of LDHA through hydrophobic interactions and hydrogen bonds. Molecular dynamic simulations tested the stability of the fisetin-LDHA complex over 100 ns and showed fisetin's high binding affinity to LDHA, maintaining strong hydrogen bonds. The binding energy of fisetin with LDHA was -33.928 kcal/mol, indicating its effectiveness as an LDHA inhibitor. Consequently, flavonoids identified as strong inhibitors could be potential cancer treatment sources through LDHA inhibition. Fisetin, quercetin 3-beta-D-glucoside, quercetin 3-galactoside, and luteolin are shown to exhibit powerful inhibition effects, with IC50 values between 0.066 and 1.792 mu M. The details of possible interactions with the active site of lactate dehydrogenase A were analyzed by molecular docking and molecular dynamics simulation. image
Açıklama
Anahtar Kelimeler
enzyme inhibition; flavonoids; lactate dehydrogenase A; molecular docking; molecular dynamics
Kaynak
Archiv Der Pharmazie
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
357
Sayı
9
