Homocysteine thiolactonase activity in coronary atherosclerosis

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dc.contributor.authorAltinay, Aylin
dc.contributor.authorKisa, Ucler
dc.contributor.authorTulmac, Murat
dc.contributor.authorOzkan, Yesim
dc.date.accessioned2021-01-14T18:10:21Z
dc.date.available2021-01-14T18:10:21Z
dc.date.issued2020
dc.departmentKKÜ
dc.description.abstractAim: Discovery of paraoxonase natural substrate, homocysteine thiolactone, shed more light on the protective role of paraoxonase and toxicity of homocysteine. Since homocysteine thiolactone and paraoxon were hydrolyzed at different sites in the PON protein, the aim of this study was to investigate the Hcy-thiolactonase (HTase). This study was undertaken to ascertain whether low the Hcy-thiolactonase acitivity is associated with paraoxonase activity and to clarify its relation with ox-LDL and total plasma homocysteine levels in coronary artery diseasase. Material and Methods: Forty-six subjects undergoing coronary angiography for suspected coronary artery disease were included. Depending on angiography results, 14 subjects with normal coronary arteries according to Gensini scoring were selected as a control group. Serum homocysteine thiolactonase and paraoxonase activities were measured spectrophotometrically. Homocysteine, ox-LDL levels were measured with ELISA methods. Results: A significant decrease in HTase activity and a significant increase in ox-LDL levels were observed in patients compared with controls (p=0.040, p=0.037, respectively). Homocysteine levels and paraoxonase activity did not show any statistically significant difference between groups. Positive correlations between HTase and paraoxonase activities were observed in study groups (rs=0.742, p=0.004 for control, rs=0.494, p=0.01 for patient). Discussion: HTase activity decreased in coronary artery disease in spite of unchanged paraoxonase activity and is associated with a higher level of ox-LDL. N-homocysteinylation of HDL changes the properties of apolipoprotein, which could affect the enzymatic activities. When considering a relationship between HTase activity and HDL levels, correlation observed in our study confirms a possible consequence of low PONs homocysteine thiolactonase activity.en_US
dc.description.sponsorshipGazi University Research FundGazi Universityen_US
dc.description.sponsorshipThis study was supported by Gazi University Research Fund (Protocol no: 02/2006-13). The funders had no role in study design, data collection, and analysis, decision to publish, or preparation of the manuscript.en_US
dc.identifier.citationAylin Altınay, Ucler Kısa, Murat Tulmac, Yesim Ozkan. Homocysteine thiolactonase activity in coronary atherosclerosis. Ann Clin Anal Med 2020;11(6):634-638.en_US
dc.identifier.doi10.4328/ACAM.20168
dc.identifier.endpage638en_US
dc.identifier.issn2667-663X
dc.identifier.issue6en_US
dc.identifier.startpage634en_US
dc.identifier.urihttps://doi.org/10.4328/ACAM.20168
dc.identifier.urihttps://hdl.handle.net/20.500.12587/12536
dc.identifier.volume11en_US
dc.identifier.wosWOS:000582489100021
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherBAYRAKOL MEDICAL PUBLISHERen_US
dc.relation.ispartofANNALS OF CLINICAL AND ANALYTICAL MEDICINE
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHomocysteineen_US
dc.subjectHomocysteine Thiolactoneen_US
dc.subjectParaoxonaseen_US
dc.subjectOxide LDLen_US
dc.subjectGensini scoreen_US
dc.titleHomocysteine thiolactonase activity in coronary atherosclerosisen_US
dc.typeArticle

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