Activity of 3 beta-hydroxysteroid dehydrogenase associated with progesterone production in bovine granulosa cells cultured under different concentrations of serum, insulin-like growth factor I, and gonadotropin

Basit Öğe Kaydı
dc.contributor.authorSimsek, Ozkan
dc.contributor.authorMihm, Monika
dc.date.accessioned2020-06-25T18:12:26Z
dc.date.available2020-06-25T18:12:26Z
dc.date.issued2014
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThree-beta-hydroxysteroid dehydrogenase (3 beta-HSD) is the enzyme responsible for progesterone production. This study aimed to determine whether 3 beta-HSD activity can be shown to reflect progesterone production by granulosa cells cultured under different serum conditions and follicle stimulating hormone (FSH), luteinising hormone (LH), and insulin-like growth factor I (IGF-I) concentrations. Large bovine follicles were dissected from abattoir ovaries to recover granulosa cells. Cells were washed, stained for viability, and plated for 48 h in basic medium with or without 5% foetal calf serum (FCS). Subsequently, cells were exposed to FSH (1 ng/mL), LH (10 or 100 ng/mL), or FSH (1 ng/mL) + IGF-I (1 or 10 ng/mL) in a serum-free medium for another 96 h to predict degree of luteinisation. Before and after incubation, granulosa cells were stained for 3 beta-HSD activity. The high dose of IGF-I (10 ng/mL) increased (P < 0.05) progesterone secretion over 2.5-fold compared with FSH alone or the low dose of IGF-I (1 ng/mL) in cells preincubated with FCS. This was clearly reflected by darker 3 beta-HSD staining than in cells exposed to FSH and low dose IGF-I.en_US
dc.identifier.citationŞimşek, Ö. and Mihm, M. (2014) "Activity of 3\beta-hydroxysteroid dehydrogenase associated with progesterone production in bovine granulosa cells cultured under different concentrations of serum, insulin-like growth factor I, and gonadotropin," Turkish Journal of Veterinary & Animal Sciences. 38(4),358-362.en_US
dc.identifier.doi10.3906/vet-1311-57
dc.identifier.endpage362en_US
dc.identifier.issn1300-0128
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84902284746
dc.identifier.scopusqualityQ3
dc.identifier.startpage358en_US
dc.identifier.urihttps://doi.org/10.3906/vet-1311-57
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5920
dc.identifier.volume38en_US
dc.identifier.wosWOS:000338320300003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherScientific Technical Research Council Turkey-Tubitaken_US
dc.relation.ispartofTurkish Journal Of Veterinary & Animal Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject3 beta-Hydroxysteroid dehydrogenaseen_US
dc.subjectbovine granulosa cellsen_US
dc.subjectprogesteroneen_US
dc.subjectFSHen_US
dc.subjectLHen_US
dc.subjectIGF-Ien_US
dc.titleActivity of 3 beta-hydroxysteroid dehydrogenase associated with progesterone production in bovine granulosa cells cultured under different concentrations of serum, insulin-like growth factor I, and gonadotropinen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Activity of 3 beta-hydroxysteroid dehydrogenase associated with progesterone production in bovine granulosa cells cultured under different concentrations of serum, insulin-like growth factor I, and gonadotropin.pdf
Boyut:
1.12 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu