Semi-interpenetrating polymer networks (semi-IPNs) for entrapment of laccase and their use in Acid Orange 52 decolorization

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dc.contributor.authorYamak, Özgür
dc.contributor.authorKalkan, N. Ayça
dc.contributor.authorAksoy, Serpil
dc.contributor.authorAltınok, Haydar
dc.contributor.authorHasırcı, Nesrin
dc.date.accessioned2020-06-25T17:48:30Z
dc.date.available2020-06-25T17:48:30Z
dc.date.issued2009
dc.departmentKırıkkale Üniversitesi
dc.descriptionHasirci, Nesrin/0000-0002-4497-0194
dc.description.abstractLaccase enzyme (L) from Trametes versicolor was entrapped in three hydrogel structures namely poly(acrylamide-N-isopropylacrylamide), P(AAm-NIPA), and semi-interpenetrating networks of poly(-acrylamide)/alginate, P(AAm)/Alg, and poly(acrylamide-N-isopropylacrylamide)/alginate, P(AAm-NIPA)/Alg. The optimum temperatures for free and all immobilized systems were found to be 40 degrees C. For free and immobilized laccase systems of P(AAm-NIPA)-L, P(AAm)/Alg-L and P(AAm-NIPA)/Alg-L, K-m values were found to be 6.7 x 10(-3), 8.8 x 10(-2), 5.5 x 10(-2) and 1,8 x 10(-2) mM: V-max values were calculated as 1.8 x 10(-3). 2.5 x 10(-2), 1.5 x 10(-2) and 6.1 x 10(-3) mM min(-1), respectively. For free and the same immobilized systems, the enzymes retained 42%, 91%. 79% and 86% of their initial activities at the end of 56 days of storage. After using the mentioned immobilized systems repeatedly 10 times, they retained 77%, 71% and 84% of their original activities, respectively. For free and the same immobilized systems, decolorization of Acid Orange 52 (AO52) in 6 h were found to be 63%, 50%, 48% and 66%, respectively. Addition of 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), ABTS, into the assay medium increased these values LIP to 73%, 73%, 74% and 75%, respectively. (C) 2008 Elsevier Ltd. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.procbio.2008.12.008
dc.identifier.endpage445en_US
dc.identifier.issn1359-5113
dc.identifier.issn1873-3298
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-62049083367
dc.identifier.scopusqualityQ1
dc.identifier.startpage440en_US
dc.identifier.urihttps://doi.org/10.1016/j.procbio.2008.12.008
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4473
dc.identifier.volume44en_US
dc.identifier.wosWOS:000265128300010
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofProcess Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSemi-interpenetrating polymer networksen_US
dc.subjectEnzyme immobilizationen_US
dc.subjectLaccaseen_US
dc.subjectEntrapmenten_US
dc.subjectDecolorizationen_US
dc.subjectAcid Orange 52en_US
dc.titleSemi-interpenetrating polymer networks (semi-IPNs) for entrapment of laccase and their use in Acid Orange 52 decolorizationen_US
dc.typeArticle

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