Effects of high density lipoprotein containing high or low β-carotene concentrations on progesterone production and β-carotene uptake and depletion by bovine luteal cells

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dc.contributor.authorArikan, S
dc.contributor.authorRodway, RG
dc.date.accessioned2020-06-25T17:34:41Z
dc.date.available2020-06-25T17:34:41Z
dc.date.issued2000
dc.departmentKırıkkale Üniversitesi
dc.description.abstractLuteal cells were isolated from mid-luteal heifer ovaries by collagenase digestion. Cells were cultured with DMEM/Ham's F12 medium in serum pre-treated plastic culture dishes for periods of up to 11 days. As beta-carotene is almost completely insoluble in all polar solvents, it was added to cultures in either dimethyl sulphoxide (DMSO), tetrahydrofuran (THF) or as high-density lipoprotein (HDL) containing high or low beta-carotene concentrations. Medium was replaced after 24 h, thereafter medium was changed every 48 h, Treatment of cells with DMSO alone or with beta-carotene (5 mu mol/l) in DMSO both resulted in significant (P < 0.01) stimulation of progesterone production. beta-Carotene (5 mu mol/l) in THF did not alter progesterone production but 50 mu mol/l beta-carotene in TNF resulted in significant inhibition (P < 0.02) of progesterone production on days 3 and 7, Cultures were also supplemented with bovine HDL preparations containing equal concentrations of cholesterol (25 mu g/ml) but high or low beta-carotene (12.4 or 0.44 mu g/mg of cholesterol). Both HDL preparations significantly stimulated progesterone production (P < 0.001) but the high beta-carotene HDL was significantly (P < 0.02) more effective than the low beta-carotene HDL. However, when given together with bovine luteinizing hormone (bLH) or dibutyryl cAMP (dbcAMP), the high beta-carotene HDL stimulated progesterone production less than did the low NDL (P < 0.01). Uptake and depletion of beta-carotene by luteal cells were also examined in culture, beta-carotene supplementation increased luteal cell beta-carotene from an initial level of 373 ng per 10(6) cells to 2,030 ng per 10(6) cells by day 6. In contrast, the levels in control cells decreased to 14% of starting values during the same period. Cells treated with HDL containing high p-carotene on day 1 or days 1 and 3 were then incubated with or without bLH or dbcAMP for a further 2 days to investigate the effect of bLH and dbcAMP on depletion of beta-carotene by luteal cells, beta-Carotene depletion in the luteal cells was significantly higher (P < 0.05) in LH- and dbcAMP-treated cells than in the control cells in both groups. These results indicate that the use of solvents such as DMSO or THF may have undesirable effects due to alteration of cell membrane permeability. Supplementation with bLH or dbcAMP may increase the metabolism of beta-carotene in luteal cells, bLH or dbcAMP together with high beta-carotene WDL may, when combined with the effect of increased beta-carotene metabolism, give less stimulation than with low beta-carotene HDL. (C) 2000 Elsevier Science B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S0378-4320(00)00122-6
dc.identifier.endpage263en_US
dc.identifier.issn0378-4320
dc.identifier.issue4en_US
dc.identifier.pmid10924828
dc.identifier.startpage253en_US
dc.identifier.urihttps://doi.org/10.1016/S0378-4320(00)00122-6
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2843
dc.identifier.volume62en_US
dc.identifier.wosWOS:000088740500001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofAnimal Reproduction Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcattle-feeding and nutritionen_US
dc.subjectbeta-caroteneen_US
dc.subjectluteal cellsen_US
dc.subjectlipoproteinen_US
dc.subjectprogesteroneen_US
dc.titleEffects of high density lipoprotein containing high or low β-carotene concentrations on progesterone production and β-carotene uptake and depletion by bovine luteal cellsen_US
dc.typeArticle

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