Preparation of methacrylamide grafted and dye-ligand immobilized PET fibers: Studies of adsorption and purification of lysozyme

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dc.contributor.authorKarakisla, Meral
dc.contributor.authorBayramoglu, Guelay
dc.contributor.authorArica, M. Yakup
dc.date.accessioned2020-06-25T17:44:32Z
dc.date.available2020-06-25T17:44:32Z
dc.date.issued2008
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIn this study, novel affinity chromatographic fibers was prepared from methacrylamide grafted poly(ethylene terephthalate), PET-g-pMAA, using benzoyl-peroxide as an initiator. A dye ligand (i.e., Procion Brown) as a ligand was then covalently immobilized on the different amount of pMAAm grafted PET fibers, (PET-g-pMAAm-PB). The fibers were characterized by surface area measurement, infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). Adsorptive properties of the composite fibers were tested using a model protein (i.e., lysozyme). To achieve these purposes, the influence of pH, ionic strength, initial lysozyme concentration, and temperature on adsorption system has been investigated and evaluated. A maximum lysozyme adsorption PET-g-pMAAm-PB fiber was obtained as 43.9 mg g(-1) at pH 7.5. The experimental equilibrium data obtained for lysozyme adsorption onto PET-g-pMAAm-PB fibers fitted well to the Langmuir isotherm model. The result of kinetic analyzed for lysozyme adsorption onto affinity fibers showed that the second-order rate equation was favorable. The purity of the eluted lysozyme, as determined by HPLC, was 84% with recovery 73% for PET-g-pMAAm-PB fiber. Experiments on regeneration and dynamic adsorption were also performed. It appears that PET-g-pMAAm-PB fibers can be applied for lysozyme separation without causing any denaturation. (C) 2008 Wiley Periodicals, Inc.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/app.27698
dc.identifier.endpage3323en_US
dc.identifier.issn0021-8995
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-43249120459
dc.identifier.scopusqualityQ2
dc.identifier.startpage3313en_US
dc.identifier.urihttps://doi.org/10.1002/app.27698
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4135
dc.identifier.volume108en_US
dc.identifier.wosWOS:000255043100069
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherJohn Wiley & Sons Incen_US
dc.relation.ispartofJournal Of Applied Polymer Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPET fibersen_US
dc.subjectmethacrylamideen_US
dc.subjectgraft copolymerizationen_US
dc.subjectdye liganden_US
dc.subjectadsorptionen_US
dc.subjectlysozymeen_US
dc.subjectisotherm modelen_US
dc.subjectkineticsen_US
dc.titlePreparation of methacrylamide grafted and dye-ligand immobilized PET fibers: Studies of adsorption and purification of lysozymeen_US
dc.typeArticle

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