Immobilization of glucose oxidase and urease in hydrogel matrices

Basit Öğe Kaydı
dc.contributor.authorGurel, I
dc.contributor.authorArica, MY
dc.contributor.authorHasirci, V
dc.date.accessioned2020-06-25T17:34:36Z
dc.date.available2020-06-25T17:34:36Z
dc.date.issued1997
dc.departmentKırıkkale Üniversitesi
dc.description.abstractImmobilization of glucose oxidase and urease in hydrogels of 2-hydroxyethyl methacrylae, and N-vinyl pyrrolidone (NVP) was achieved by irradiation (using UV kand gamma-rays). The effect of radiation on entrapment efficiencies, retention of activities and swelling rates was obtained. To optimize the system, duration of exposure, reaction temperature, co-monomer concentrations, initiator and cross linker compositions were varied. The repeated reusability of the generated products was also tested. It was found that gamma-irradiation at very low temperatures did not have a detrimental effect on the enzyme activity. Incorporation of more hydrophilic monomer (NVP), influenced matrix morphology and entrapment efficiencies and retained enzyme activities positively Repeated use studies revealed that there is a gradual loss (higher in the NVPL incorporated group) of activity following 10 runs. With the use of the present approach it is believed that the construction of biosensors using hydrogels of arious forms is possible.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.endpage393en_US
dc.identifier.issn1010-7614
dc.identifier.issue4en_US
dc.identifier.startpage387en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2792
dc.identifier.volume21en_US
dc.identifier.wosWOS:000072327400023
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherScientific Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleImmobilization of glucose oxidase and urease in hydrogel matricesen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
Ä°sim:
Immobilization of glucose oxidase and urease in hydrogel matrices.pdf
Boyut:
812.06 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
Ä°ndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu