Investigation of therapeutic effects of calcium dobesilate in cerebral hypoxia/ reperfusion injury in rats

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dc.contributor.authorOzdemir, Alemiddin
dc.contributor.authorOgden, Mustafa
dc.contributor.authorKartal, Bahar
dc.contributor.authorCeylan, Asli Fahriye
dc.contributor.authorYuksel, Ulas
dc.contributor.authorBakar, Bulent
dc.date.accessioned2025-01-21T16:28:00Z
dc.date.available2025-01-21T16:28:00Z
dc.date.issued2023
dc.departmentKırıkkale Üniversitesi
dc.description.abstractObjectives: Cerebral stroke is a serious clinical condition in which oxidative stress, inflammation, necrosis, apoptosis, and autophagy play important roles in its pathogenesis. This study investigated the neuroprotective and healing effects of calcium dobesilate (CD) on cerebral hypoxia/reperfusion injury in rats. Methods: Forty Wistar albino male rats, each weighing 300–350 g, were separated into the Control group (no surgery and no pharmacological agent was administered); Sham-A group (only surgery was performed); DBL-A group (surgery was performed and CD 100 mg/kg/day was administered intraperitoneally for 3 days); Sham-C group (only surgery was performed); and DBL-C group (surgery was performed and 100 mg/kg/day CD was administered intraperitoneally for 10 days). Under sedation anesthesia, the bilateral common carotid arteries of all rats except the Control group were clipped for 30 min. After 4 h, the CD was given to the relevant groups, and then, all subjects were euthanized at scheduled times. The brain of each animal was removed for histopathological (hematoxylin and eosin staining), immunohistochemical (beclin-1, anti-MHC class II and anti-CD-68 staining), and biochemical (TNF, IL-1?, IL-6, caspase-3, GSH/GSSG, malondialdehyde, protein carbonyl, LC3II/LC3I, and beclin-1 levels) evaluations. Results: It was observed that CD could reduce necrosis and mitigate polarization of microglia to the M1 phenotype, autophagy, free oxygen radicals, protein carbonylation, lipid peroxidation, IL-1?, IL6, TNF, caspase-3, beclin-1, and LC3II/LC3I levels in acute and chronic periods of hypoxia/reperfusion injury. Conclusion: From these results, it was observed that CD treatment could reduce neuronal necrosis and create anti-inflammatory, anti-edema, anti-oxidant, anti-apoptotic, and anti-autophagic effects in hypoxia/reperfusion injury in rats. © 2022 Informa UK Limited, trading as Taylor & Francis Group.
dc.description.sponsorshipKırıkkale Üniversitesi, KU, (2020/41)
dc.identifier.doi10.1080/01616412.2022.2159109
dc.identifier.endpage487
dc.identifier.issn0161-6412
dc.identifier.issue5
dc.identifier.pmid36548871
dc.identifier.scopus2-s2.0-85145104613
dc.identifier.scopusqualityQ3
dc.identifier.startpage472
dc.identifier.urihttps://doi.org/10.1080/01616412.2022.2159109
dc.identifier.urihttps://hdl.handle.net/20.500.12587/23440
dc.identifier.volume45
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor and Francis Ltd.
dc.relation.ispartofNeurological Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectapoptosis; autophagy; Calcium dobesilate; cerebral hypoxia/reperfusion injury; inflammation
dc.titleInvestigation of therapeutic effects of calcium dobesilate in cerebral hypoxia/ reperfusion injury in rats
dc.typeArticle

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