The Efficacy of Electrochemotherapy with Dacarbazine on Melanoma Cells

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dc.authoridSenturk, Fatih/0000-0002-2436-3362
dc.contributor.authorCoskun, Alaaddin
dc.contributor.authorKayhan, Handan
dc.contributor.authorSenturk, Fatih
dc.contributor.authorEsmekaya, Meric Arda
dc.contributor.authorCanseven, Ayse Gulnihal
dc.date.accessioned2025-01-21T16:45:28Z
dc.date.available2025-01-21T16:45:28Z
dc.date.issued2024
dc.departmentKırıkkale Üniversitesi
dc.description.abstractElectrochemotherapy (ECT) involves locally applying electrical pulses to permeabilize cell membranes, using electroporation (EP). This process enhances the uptake of low-permeant chemotherapeutic agents, consequently amplifying their cytotoxic effects. In melanoma treatment, dacarbazine (DTIC) is a cornerstone, but it faces limitations because of poor cell membrane penetration, necessitating the use of high doses, which, in turn, leads to increased side effects. In our study, we investigated the effects of DTIC and EP, both individually and in combination, on the melanoma cell line (SK-MEL-30) as well as human dermal fibroblasts (HDF) using in vitro assays. First, the effects of different DTIC concentrations on the viability of SK-MEL-30 and HDF cells were determined, revealing that DTIC was more effective against melanoma cells at lower concentrations, whereas its cytotoxicity at 1000 mu M was similar in both cell types. Next, an ideal electric field strength of 1500 V/cm achieved a balance between permeability (84%) and melanoma cell viability (79%), paving the way for effective ECT. The combined DTIC-EP (ECT) application reduced IC50 values by 2.2-fold in SK-MEL-30 cells and 2.7-fold in HDF cells compared with DTIC alone. In conclusion, ECT not only increased DTIC's cytotoxicity against melanoma cells but also affected healthy fibroblasts. These findings emphasize the need for cautious, targeted ECT management in melanoma therapy.
dc.description.sponsorshipGazi University [01/2015-03]
dc.description.sponsorshipThe research was supported by the Scientific Research Projects (01/2015-03) at Gazi University. The authorsexpress their gratitude for the assistance provided by Dr.Ongun Onaran, Dr. Sanem Ozcelik, and Dr. Mehmed ZahidTuysuz.
dc.identifier.doi10.1089/bioe.2023.0041
dc.identifier.endpage125
dc.identifier.issn2576-3105
dc.identifier.issn2576-3113
dc.identifier.issue2
dc.identifier.pmid39119570
dc.identifier.startpage118
dc.identifier.urihttps://doi.org/10.1089/bioe.2023.0041
dc.identifier.urihttps://hdl.handle.net/20.500.12587/25653
dc.identifier.volume6
dc.identifier.wosWOS:001201389200001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMary Ann Liebert, Inc
dc.relation.ispartofBioelectricity
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectelectrochemotherapy; dacarbazine; melanoma cell lines; human dermal fibroblasts
dc.titleThe Efficacy of Electrochemotherapy with Dacarbazine on Melanoma Cells
dc.typeArticle

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