Covalent immobilization of invertase on chemically activated poly (styrene-2-hydroxyethyl methacrylate) microbeads

Basit Öğe Kaydı
dc.contributor.authorAltinok, Haydar
dc.contributor.authorAksoy, Serpil
dc.contributor.authorTumturk, Hayrettin
dc.contributor.authorHasirci, Nesrin
dc.date.accessioned2020-06-25T17:44:35Z
dc.date.available2020-06-25T17:44:35Z
dc.date.issued2008
dc.departmentKırıkkale Üniversitesi
dc.descriptionHASIRCI, Nesrin/0000-0002-4497-0194
dc.description.abstractA carrier for invertase enzyme was synthesized from styrene (S) and 2- hydroxyethyl methacrylate (HEMA) in the form of microbeads. These poly (styrene-2-hydroxyethyl methacrylate), P(S-HEMA) microbeads were activated by epichlorohydrin (ECH) treatment for covalent immobilization. The free and immobilized invertase were assayed in the hydrolysis of sucrose to glucose, and the obtained results were compared. The optimum pH was 4.5 for free and 5.5 for immobilized invertase. The optimum temperature of invertase shifted from 45C to 55C upon immobilization. For free and immobilized enzymes, kinetic parameters were calculated as 4.1 x 10(-3) mol L(-1)and 9.2 x 10(-3) mol L(-1)for K-m, and 6.6 x 10(-2) mol L-1 min(-1)and 4.1 x 10(-1) mol L-1 min(-1)for V-max, respectively. After 1 month of storage at 4C, free enzyme retained 36% of its initial activity, while for the ECH-activated P(S-HEMA) immobilized enzyme, P(S-HEMA)-E, this value was observed as 67%. In repeated batch use, i.e., 20 times in 3 days, 78% retention of the initial activity was observed for P(S-HEMA)-E system.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1111/j.1745-4514.2008.00156.x
dc.identifier.endpage315en_US
dc.identifier.issn0145-8884
dc.identifier.issn1745-4514
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-44349182547
dc.identifier.scopusqualityQ1
dc.identifier.startpage299en_US
dc.identifier.urihttps://doi.org/10.1111/j.1745-4514.2008.00156.x
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4148
dc.identifier.volume32en_US
dc.identifier.wosWOS:000256156800002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Food Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleCovalent immobilization of invertase on chemically activated poly (styrene-2-hydroxyethyl methacrylate) microbeadsen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Covalent immobilization of invertase on chemically activated poly (styrene-2-hydroxyethyl methacrylate) microbeads.pdf
Boyut:
262.48 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu