Preparation of ion-exchange beads based on poly (methacrylic acid) brush grafted chitosan beads: Isolation of lysozyme from egg white in batch system

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dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorEkici, Gülsüm
dc.contributor.authorBesirli, Necati
dc.contributor.authorArıca, M. Yakup
dc.date.accessioned2020-06-25T17:43:35Z
dc.date.available2020-06-25T17:43:35Z
dc.date.issued2007
dc.description.abstractPoly(methacrylic acid) brush grafted crosslinked-chitosan (chitosan-g-poly(MAA)) beads were prepared in two sequential steps: in the first step, chitosan beads were prepared by phase-inversion technique and then were crosslinked with epichlorohydrin under alkaline condition; in the second step, the graft copolymerization of methacrylic acid onto the chitosan beads was initiated by ammonium persulfate (APS) under nitrogen atmosphere. The chitosan-g-poly(MAA) beads were first used as an ion exchange support for adsorption of lysozyme (LYZ) from aqueous solution. The influence of pH, equilibrium time, ionic strength and initial LYZ concentration on the adsorption capacity of the chitosan-g-poly(MAA) ion-exchange beads has been investigated in a batch system. Maximum LYZ adsorption onto chitosan-g-poly(MAA) beads was found to be 65.7 mg/g at pH 6.0. The experimental equilibrium data obtained LYZ adsorption onto chitosan-g-poly(MAA) ion-exchange beads fitted well to the Langmuir isotherm model. Kinetics parameters of this adsorption system were also analyzed by using the equilibrium experimental data. The result of kinetic analyzed for LYZ adsorption onto ion-exchange beads showed that the second order rate equation was favourable. Finally, the chitosan-g-poly(MAA) ion-exchange beads were used for the purification of LYZ from egg white in batch system and the purity of the eluted LYZ from ion-exchange chitosan-g-poly(MAA) beads was determined as 94% by HPLC from single step purification. (c) 2007 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.colsurfa.2007.05.067
dc.identifier.endpage77en_US
dc.identifier.issn0927-7757
dc.identifier.issue1-3en_US
dc.identifier.scopus2-s2.0-34848832902
dc.identifier.scopusqualityQ1
dc.identifier.startpage68en_US
dc.identifier.urihttps://doi.org10.1016/j.colsurfa.2007.05.067
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3813
dc.identifier.volume310en_US
dc.identifier.wosWOS:000250697100009
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofColloids And Surfaces A-Physicochemical And Engineering Aspects
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectchitosan-g-poly(MAA) beadsen_US
dc.subjection-exchange chromatographyen_US
dc.subjectgraft copolymerizationen_US
dc.subjectadsorptionen_US
dc.subjectlysozymeen_US
dc.subjectthermodynamic parametersen_US
dc.titlePreparation of ion-exchange beads based on poly (methacrylic acid) brush grafted chitosan beads: Isolation of lysozyme from egg white in batch systemen_US
dc.typeArticle

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