Amperometric glucose sensor based on the glucose oxidase enzyme immobilized on graphite rod electrode modified with Fe3O4-CS-Au magnetic nanoparticles
Yükleniyor...
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Heidelberg
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this report, an amperometric detection method for blood glucose level was developed benefiting from glucose oxidase (GO) enzyme immobilization on nanoparticle modified graphite rod (GR) electrode. Fe3O4-CS-Au magnetic nanoparticles were synthesized, characterized by TEM, UV-Vis, magnetometry, FTIR, and zeta potential measurements and used for the modification of GR electrode. This modified electrode was used for the detection of glucose level amperometrically at 0.6 V. The obtained calibration graph was linear in the range of 5-30mM glucose concentration with a coefficient of determination (R-2) 0.9971. The limit of detection (LOD) and limit of quantification (LOQ) values were calculated as 0.55 and 1.83mM, respectively. The modified GR electrode showed excellent selectivity in the presence of dopamine, ascorbic acid, and uric acid. The applicability of the developed method was examined in real blood samples by comparing the results obtained from commercial glucose sensor. This novel glucose detection method exhibited fast amperometric response, long storage time, and good selectivity.
Açıklama
Cetin, Demet/0000-0003-1186-4229
Anahtar Kelimeler
Glucose, Electrochemical sensor, Magnetic nanoparticle, Graphite rod, Chitosan
Kaynak
Ionics
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
24
Sayı
12
Künye
closedAccess
