Biodegradation of 2,4,6-Trinitrotoluene (TNT) with Bacteria Isolated from TNT-polluted Waste Pink Water
Yükleniyor...
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Budapest Univ Technology Economics
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, bacterial strains that can use TNT as a nitrogen source isolated from TNT contaminated pink water. We isolated 5 bacterial strains and the isolated bacteria were cultured in medium containing TNT and TNT degradation capacities of isolates were determined by spectrophotometric analysis. According to the results of the analysis that have done, 3 bacterial isolates that have high TNT degradation capacity were selected and the isolates were identified with firstly Gram-staining then with 16S rRNA sequence analysis method. According to the sequence of 16S rRNA, water isolates were identified as Stenotrophomonas maltophilia (SU K2), Klebsiella pneumoniae (SU K3), Raoultella planticola (SU K4). During the TNT degradation studies, at the end of 24 h incubation time, in the medium containing 100 mg/L TNT, TNT degradation rate for SU K2, SU K3 and SU K4 were determined 70 %, 96 % and 93 % respectively. 4-aminodinitrotoluene and 2-aminodinitrotoluene accumulations were detected in the culture medium of all isolates as intermediate products formed during the degradation of TNT by HPLC analysis. Additionally, nitrite accumulation was detected in the culture medium of all isolates and the influence of temperature and pH on the degradation of TNT was also investigated. It was determined that SU K2 isolates have the highest TNT degradation capacity at 35 degrees C, the others have at 30 degrees C and all isolates degraded TNT fastest at pH 7. The results of the study show that the new isolates can be useful for the removal of TNT in a wastewater treatment system.
Açıklama
GUN GOK, Zehra/0000-0001-6426-0395
Anahtar Kelimeler
biodegradation, trinitrotoluene, contaminated water, isolation of bacteria
Kaynak
Periodica Polytechnica-Chemical Engineering
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
63
Sayı
3
Künye
Gün Gök, Z., İnal, M., Yiğitoğlu, M.(2019). “Biodegradation of 2,4,6-Trinitrotoluene (TNT) with Bacteria Isolated from TNT-polluted Waste Pink Water”, Periodica Polytechnica Chemical Engineering, 63(3), 459-468.
