Immobilization of glucose isomerase in surface-modified alginate gel beads
Yükleniyor...
Tarih
2008
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, glucose isomerase enzyme was entrapped into modified and nonmodified calcium alginate gel beads. Various characteristics of free and immobilized enzymes such as the optimum pH, temperature and dependence of activity on storage and operational stability were evaluated. The optimum pH and temperature of free and immobilized glucose isomerase were found to be the same values as 7.5 and 60C, respectively. For free and immobilized enzymes, kinetic parameters were calculated as 1.79 x 10(-2) and 8.27 x 10(-3) mol/L for Km, and 2.39 x 10(-3) and 6.03 x 10(-3) mol/L min for V-max, respectively. After 42 days of storage at 4C, free enzyme retained 56% of its initial activity, while for the immobilized enzyme, this value was observed as 86%. The immobilized samples were used repeatedly 22 times by retaining more than 85% of their initial activity.
Açıklama
Hasirci, Nesrin/0000-0002-4497-0194; Demirel, Gokhan/0000-0002-9778-917X
Anahtar Kelimeler
Kaynak
Journal Of Food Biochemistry
WoS Q Değeri
Q3
Scopus Q Değeri
Q1
Cilt
32
Sayı
2
Künye
closedAccess
