Inhaler Steroid Use Changes Oral and Airway Bacterial and Fungal Microbiome Profile in Asthma Patients

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dc.authoridPur Ozyigit, Leyla/0000-0002-7113-9988
dc.authoridKoycu Buhari, Gozde/0000-0002-0787-0370
dc.authoridGOKSEL, Ozlem/0000-0003-1121-9967
dc.contributor.authorOguzulgen, Kivilcim
dc.contributor.authorOzturk, Ayse Bilge
dc.contributor.authorBacceoglu, Ayse
dc.contributor.authorAydin, Omur
dc.contributor.authorKoeycue Buhari, Gozde
dc.contributor.authorDamadoglu, Ebru
dc.contributor.authorOner Erkekol, Ferda
dc.date.accessioned2025-01-21T16:41:49Z
dc.date.available2025-01-21T16:41:49Z
dc.date.issued2024
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIntroduction: The full spectrum of bacterial and fungal species in adult asthma and the effect of inhaled corticosteroid use is not well described. The aim was to collect mouthwash and induced sputum samples from newly diagnosed asthma patients in the pretreatment period and in chronic asthma patients while undergoing regular maintenance inhaled corticosteroid therapy, in order to demonstrate the bacterial and fungal microbiome profile. Methods: The study included 28 asthmatic patients on inhaler steroid therapy, 25 steroid-naive asthmatics, and 24 healthy controls. Genomic DNA was isolated from induced sputum and mouthwash samples. Analyses were performed using bacterial primers selected from the 16S rRNA region for the bacterial genome and panfungal primers selected from the 5.8S rRNA region for the fungal genome. Results: Dominant genera in mouthwash samples of steroid-naive asthmatics were Neisseria, Haemophilus, and Rothia. The oral microbiota of asthmatic patients on inhaler steroid treatment included Neisseria, Rothia, and Veillonella species. Abundant genera in induced sputum samples of steroid-naive asthma patients were Actinomyces, Granulicatella, Fusobacterium, Peptostreptococcus, and Atopobium. Sputum microbiota of asthma patients taking inhaler steroids were dominated by Prevotella and Porphyromonas. Mucor plumbeus and Malassezia restricta species were abundant in the airways of steroid-naive asthma patients. Choanephora infundibulifera and Malassezia restricta became dominant in asthma patients taking inhaled steroids. Conclusion: The oral and airway microbiota consist of different bacterial and fungal communities in healthy and asthmatic patients. Inhaler steroid use may influence the composition of the oral and airway microbiota.
dc.description.sponsorshipTurkish Thoracic Society
dc.description.sponsorshipThe study was funded by Turkish Thoracic Society. Grant number is not applicable.
dc.identifier.doi10.1159/000531866
dc.identifier.endpage19
dc.identifier.issn1018-2438
dc.identifier.issn1423-0097
dc.identifier.issue1
dc.identifier.pmid37844548
dc.identifier.startpage10
dc.identifier.urihttps://doi.org/10.1159/000531866
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24958
dc.identifier.volume185
dc.identifier.wosWOS:001091143600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherKarger
dc.relation.ispartofInternational Archives of Allergy and Immunology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectAirway; Airway microbiome; Asthma; Inhaler steroid; Lung microbiome; Microbiome; Oral microbiome; Steroid
dc.titleInhaler Steroid Use Changes Oral and Airway Bacterial and Fungal Microbiome Profile in Asthma Patients
dc.typeArticle

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