In vitro comparison of nanofibrillar and macroporous-spongious composite tissue scaffolds for periodontal tissue engineering

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dc.authoridBuber, Esra/0000-0002-9707-4270
dc.authoridBayari, Sevgi/0000-0001-9265-1414
dc.contributor.authorSahbazoglu, Kemal Burak
dc.contributor.authorDemirbilek, Murat
dc.contributor.authorBayari, Sevgi Haman
dc.contributor.authorBuber, Esra
dc.contributor.authorToklucu, Selcuk
dc.contributor.authorTurk, Mustafa
dc.contributor.authorKarabulut, Erdem
dc.date.accessioned2025-01-21T16:41:44Z
dc.date.available2025-01-21T16:41:44Z
dc.date.issued2022
dc.departmentKırıkkale Üniversitesi
dc.description.abstractPurpose/Aim of the study: The ultimate goal of periodontal treatment is to regenerate the lost periodontal tissues. The interest in nanomaterials in dentistry is growing rapidly and has focused on improvements in various biomedical applications, such as periodontal regeneration and periodontal tissue engineering. To enhance periodontal tissue regeneration, hydroxyapatite (HA) was used in conjunction with other scaffold materials, such as Poly lactic-co-glycolic-acid (PLGA) and collagen (C). The main target of this study was to compare the effects of nano and macrostructures of the tissue scaffolds on cell behavior in vitro for periodontal tissue engineering. Materials and Methods: Nanofibrillar and macroporous-spongious composite tissue scaffolds were produced using PLGA/C/HA. Subgroups with BMP-2 signal molecule and without HA were also created. The scaffolds were characterized by FTIR, SEM/EDX techniques, and mechanical tests. The scaffolds were compared in the periodontal ligament (PDL) and MCT3-E1 cell cultures. The cell behaviors; adhesions by SEM, proliferation by WST-1, differentiation by ALP and mineralization with Alizarin Red Tests were determined. Results: Cell adhesion and mineralization were higher in the nanofibrillar scaffolds compared to the macroporous-spongious scaffolds. Macroporous-spongious scaffolds seemed better for the proliferation of PDL cells and differentiation of MC3T3-E1-preosteoblastic cells, while nanofibrillar scaffolds were more convenient for the differentiation of PDL cells and proliferation of MC3T3-E1-preosteoblastic cells. Conclusions: In general, nanofibrillar scaffolds showed more favorable results in cell behaviors, compared to the macroporous-spongious scaffolds, and mostly, BMP-2 and HA promoted the activities of the cells.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [113S534]
dc.description.sponsorshipThis work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK), Grant Number: 113S534.
dc.identifier.doi10.1080/03008207.2021.1912029
dc.identifier.endpage197
dc.identifier.issn0300-8207
dc.identifier.issn1607-8438
dc.identifier.issue2
dc.identifier.pmid33899631
dc.identifier.startpage183
dc.identifier.urihttps://doi.org/10.1080/03008207.2021.1912029
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24936
dc.identifier.volume63
dc.identifier.wosWOS:000644220200001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofConnective Tissue Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectPeriodontal tissue engineering; periodontal regeneration; nanocomposite-tissue scaffolds; PLGA tissue scaffolds; PDL cell cultures
dc.titleIn vitro comparison of nanofibrillar and macroporous-spongious composite tissue scaffolds for periodontal tissue engineering
dc.typeArticle

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