Anaerobic glycolysis is the main pathway for energy generation in HI-60 acute promyelocytic leukemia cells

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dc.contributor.authorBoyunaga H.
dc.contributor.authorKeles H.
dc.contributor.authorKenar L.
dc.contributor.authorUral A.U.
dc.contributor.authorAvcu F.
dc.date.accessioned2020-06-25T15:14:34Z
dc.date.available2020-06-25T15:14:34Z
dc.date.issued2010
dc.departmentKırıkkale Üniversitesi
dc.description.abstractAim In physiological conditions, normal cells use mainly the glycolytic aerobic pathway to provide energy. However, most cancer cells utilize anaerobic glycolytic way for energy generation. Aim of this study was to investigate the carbohydrate metabolic pathways of HL-60 acute promyelocytic leukemia cells for energy production. Material and Methods Leukemia cells as well as normal leukocytes were incubated with radiolabelled glucose in aerobic and anaerobic conditions and glycogen consumptions and the ratios of radiolabelled glucose catabolized into CO2 or lactate, that is, the rates of aerobic or anaerobic glycolysis, were determined. Results The glycogen consumption was significantly higher in aerobic leukemia cell culture than normal leukocyte culture (p<0.01). The rate of anaerobic glycolysis was 93.8% in leukemia cells in aerobic conditions and it increased to 96.6% while utilization of glycogen increased by 7.31% in anaerobic conditions. Conclusion In conclusion, principally anaerobic glycolysis is effective for energy generation in HL-60 promyelocytic leukemia cells. This result may be important for the development of new therapeutic approaches in the treatment of promyelocytic leukemia, requiring further comprehensive studies.en_US
dc.identifier.doi10.4328/JCAM.44
dc.identifier.endpage8en_US
dc.identifier.issn13090720
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-79952261487
dc.identifier.scopusqualityN/A
dc.identifier.startpage5en_US
dc.identifier.urihttps://doi.org/10.4328/JCAM.44
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2143
dc.identifier.volume1en_US
dc.identifier.wosWOS:000215540900002
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isotr
dc.publisherDerman Medical Publishingen_US
dc.relation.ispartofJournal of Clinical and Analytical Medicine
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEnergy Metabolismen_US
dc.subjectGlycogen Degradationen_US
dc.subjectHL-60 Promyelocytic Leukemia Cellen_US
dc.titleAnaerobic glycolysis is the main pathway for energy generation in HI-60 acute promyelocytic leukemia cellsen_US
dc.title.alternativeAnaerobik Glikoliz HL-60 Akut Promyelositik Lösemi Hücrelerinde Enerjinin Oluşumu için Temel Yolduren_US
dc.typeArticle

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