Polyethylenimine-grafted and HSA-immobilized poly(GMA-MMA) aff inity adsorbents for bilirubin removal

Basit Öğe Kaydı
dc.contributor.authorArıca, M. Yakup
dc.contributor.authorYalçın, Emine
dc.contributor.authorBayramoglu, G
dc.date.accessioned2020-06-25T17:40:53Z
dc.date.available2020-06-25T17:40:53Z
dc.date.issued2005
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe epoxy-group-containing microspheres from cross-linked glycidyl methacrylate and methyl methacrylate, poly(GMA-MMA), were prepared by suspension polymerisation. The epoxy groups of the poly(GMA-MMA) microspheres were used for grafting with an anionic polymer polyethylenimine (PEI) to prepare non-specific affinity adsorbents (poly (GMA-MMA)-PEI) for bilirubin removal. The specificity of the poly(GMA-MMA) -PEI adsorbent to bilirubin was further increased by immobilization of human serum albumin (HSA) via adsorption onto PEI-grafted poly(GMA-MMA) adsorbent. Various amounts of HSA were immobilized on the poly (GMA-MMA) -PEI adsorbent by changing the medium pH and initial HSA concentration. The maximum HSA content was obtained at 68.3 mg g(-1) microspheres. The effects of pH, ionic strength, temperature and initial bilirubin concentration on the adsorption capacity of both adsorbents were investigated in a batch system. Separation of bilirubin from human serum was also investigated in a continuous-flow system. The bilirubin adsorption on the poly(GMA-MMA)-PEI and poly(GMA-MMA)-PEI-HSA was not well described by the Langmuir model, but obeyed the Freundlich isotherm model. The poly(GMA-MMA) -PEI affinity microspheres are stable when subjected to sanitization with sodium hydroxide after repeated adsorption-desorption cycles. (C) 2004 Society of Chemical Industry.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.endpage160en_US
dc.identifier.issn0959-8103
dc.identifier.issn1097-0126
dc.identifier.issue1en_US
dc.identifier.startpage153en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3578
dc.identifier.volume54en_US
dc.identifier.wosWOS:000226036800020
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherWiley-Blackwellen_US
dc.relation.ispartofPolymer International
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbilirubinen_US
dc.subjectHSAen_US
dc.subjectPEIen_US
dc.subjectgraftingen_US
dc.subjectimmobilizationen_US
dc.subjectmicrospheresen_US
dc.subjecthydrogelsen_US
dc.titlePolyethylenimine-grafted and HSA-immobilized poly(GMA-MMA) aff inity adsorbents for bilirubin removalen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Polyethylenimine-grafted and HSA-immobilized poly(GMA-MMA) aff inity adsorbents for bilirubin removal.pdf
Boyut:
155.19 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Makale Koleksiyonu