Isolation of DNA for RAPD analysis from dry leaf material of some Hesperis L. specimens

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dc.contributor.authorAras, Sümer
dc.contributor.authorDuran, Ahmet
dc.contributor.authorYenilmez, Gülay
dc.date.accessioned2020-06-25T15:13:17Z
dc.date.available2020-06-25T15:13:17Z
dc.date.issued2003
dc.departmentKırıkkale Üniversitesi
dc.description.abstractAn improved protocol for the isolation of DNA from dry material of some Hesperis specimens is described. The isolated DNA is suitable for random amplification of polymorphic DNA (RAPD) analysis. Different DNA extraction protocols were examined to determine which might yield DNA from dry leaf tissue of Hesperis specimens. The methods examined include the protocols with hexadecyltrimethylammonium bromide (CTAB) described by Doyle and Doyle (1987); sodium dodecyl sulfate (SDS) by Dellaporta et al. (1983); and CTAB and SDS, the modified minipreparation, by Dellaporta et al (1983). None of these procedures yielded DNA of suitable purity for RAPD assay. We established an improved procedure involving CTAB and enzymatic digestion of proteins and RNA. The recovery of DNA with an average yield of 25 mg/g of leaf material was possible with this procedure. RAPD bands, which could be used to distinguish among Hesperis specimens, were generated.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.endpage461fen_US
dc.identifier.issn07359640
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-4544301642
dc.identifier.scopusqualityQ2
dc.identifier.startpage461aen_US
dc.identifier.urihttps://hdl.handle.net/20.500.12587/1716
dc.identifier.volume21en_US
dc.indekslendigikaynakScopus
dc.language.isoen
dc.relation.ispartofPlant Molecular Biology Reporter
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDNA isolationen_US
dc.subjectDry leaf materialen_US
dc.subjectHesperisen_US
dc.subjectRAPDen_US
dc.titleIsolation of DNA for RAPD analysis from dry leaf material of some Hesperis L. specimensen_US
dc.typeArticle

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