Bioengineering functional copolymers. XV. Synthesis of organoboron amide-ester branched derivatives of oligo(maleic anhydride) and their interaction with HeLa and L929 fibroblast cells

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dc.contributor.authorKahraman, Gulten
dc.contributor.authorTurk, Mustafa
dc.contributor.authorRzayev, Zakir M. O.
dc.contributor.authorUnsal, M. Elif
dc.contributor.authorSoylemez, Ernur
dc.date.accessioned2020-06-25T17:52:12Z
dc.date.available2020-06-25T17:52:12Z
dc.date.issued2011
dc.departmentKırıkkale Üniversitesi
dc.description.abstractNovel bioengineering functional organoboron oligomers were synthesized by (i) amidolysis of oligo(maleic anhydride) (OMA) with 2-aminoethyldiphenylborinate (2-AEPB), (ii) esterification of organoboron oligomer (OMA-B) with alpha-hydroxy-omega-methoxypoly(ethylene oxide) (PEO) as a compatibilizer and (iii) conjugation of organoboron PEO branches (OMA-B-PEO) with folic acid as a taggering agent. Structure and composition of the synthesized oligomers were characterized by FTIR-ART and (1)H ((13)C) NMR spectroscopy, chemical and physical analysis methods. Interaction of functional oligomers and oligomer center dot center dot center dot FA complex (OMA-B-PEO-F) with HeLa and L929 fibroblast cells were investigated by using different biochemical methods such as cytotoxicity, statistical, apoptotic and necrotic cell indexes, double staining and caspase-3 immunostaining, light and fluorescence inverted microscope analyses. It was found that citotoxisity and apoptotic/necrotic effects of oligomers significantly depend on the structure and composition of studied oligomers, and increase the following raw: OMA << OMA-B < OMA-B-PEO < OMA-B-PEO-F. A folic acid complex (MA-PEG-B-F) at 400 mu g ml(-1) (2.36 mu mol ml(-1)) concentration as a therapeutic drug exhibits minimal toxcisity toward the fibroblast cells, but influential for HeLa cells.en_US
dc.description.sponsorshipTAEKMinistry of Energy & Natural Resources - Turkey; TUBITAK (Turkish National Scientific and Technology Research Council)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TBAG-2386]en_US
dc.description.sponsorshipSupports: TAEK and TUBITAK (Turkish National Scientific and Technology Research Council) - TBAG-2386 project.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1135/cccc2010080
dc.identifier.endpage1031en_US
dc.identifier.issn0010-0765
dc.identifier.issue8en_US
dc.identifier.scopus2-s2.0-80051727398
dc.identifier.scopusqualityN/A
dc.identifier.startpage1013en_US
dc.identifier.urihttps://doi.org/10.1135/cccc2010080
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5107
dc.identifier.volume76en_US
dc.identifier.wosWOS:000298002900006
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherInst Organic Chem And Biochemen_US
dc.relation.ispartofCollection Of Czechoslovak Chemical Communications
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSynthesisen_US
dc.subjectAmidolysisen_US
dc.subjectOrganoboron oligomersen_US
dc.subjectConjugationen_US
dc.subjectCytotoxicityen_US
dc.subjectApoptotic and necrotic effectsen_US
dc.titleBioengineering functional copolymers. XV. Synthesis of organoboron amide-ester branched derivatives of oligo(maleic anhydride) and their interaction with HeLa and L929 fibroblast cellsen_US
dc.typeArticle

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