Evaluation of Burn Wound Healing Efficacy and Biocompatibility of Centella asiatica Mediated Synthesised AgNPs Loaded Hybrid Nanofiber Scaffold: In Vitro and In Vivo Studies

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dc.contributor.authorBozkaya, Ogun
dc.contributor.authorBozkaya, Esra
dc.contributor.authorEkici, Husamettin
dc.contributor.authorAlcigir, Mehmet Eray
dc.contributor.authorSahin, Yasar
dc.contributor.authorCerci, Nebahat Aytuna
dc.contributor.authorKarahan, Siyami
dc.date.accessioned2025-01-21T16:40:36Z
dc.date.available2025-01-21T16:40:36Z
dc.date.issued2024
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe aim of this study is to evaluate the cell responses, potential skin reactions during the treatment process and burn wound healing efficacy of electrospun polycaprolactone/polyethylene oxide (PCL/PEO) nanofibers (NFs) containing Centella asiatica mediated synthesized silver nanoparticles (CA-AgNPs) by in vitro and in vivo studies. Apoptosis-necrosis, genotoxicity, hemolysis, and cell attachment studies are carried out within the scope of in vitro tests, and irritation, sensitivity, and burn wound studies are carried out within the scope of in vivo tests. The apoptotic index value of CA-AgNPs-[PCL/PEO] NFs material on L929 fibroblast cells is determined as 5.0 +/- 1.0% at the highest concentration and the necrotic index value is 5.0 +/- 0.3%. Micronucleus rates (%) of NFs treated with CHO (Chinese Hamster Ovary) cells are not at genotoxic level. The hemolytic index value of NFs dressing is determined as 0.23 +/- 0.03%, The primary irritation index (PII) value of NFs wound dressing is calculated as 0.36 by irritation tests. In addition, the potential sensitization reaction of NFs extract on guinea pigs is evaluated and the sensitization score is determined as 0.9. The healing efficacy of NFs material on second-degree burn wounds compared to a commercial product is supported by pathomorphological findings.
dc.description.sponsorshipKimath;rimath;kkale University Scientific and Technological Researches Application and Research Center (KUBTUAM); Hacettepe University
dc.description.sponsorshipThe authors were grateful to K & imath;r & imath;kkale University and K & imath;r & imath;kkale University Scientific and Technological Researches Application and Research Center (KUBTUAM) for their support. In addition, this study constitutes a part of Ogun Bozkaya's doctoral dissertation studies at Hacettepe University, Institute of Science, Bioengineering doctorate program. We would like to thank Hacettepe University for their support.
dc.identifier.doi10.1002/mame.202400186
dc.identifier.issn1438-7492
dc.identifier.issn1439-2054
dc.identifier.issue12
dc.identifier.scopus2-s2.0-85201282686
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/mame.202400186
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24720
dc.identifier.volume309
dc.identifier.wosWOS:001292918800001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofMacromolecular Materials and Engineering
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241229
dc.subjectbiocompatibility; burn wound; Centella asiatica; wound dressing; wound healing
dc.titleEvaluation of Burn Wound Healing Efficacy and Biocompatibility of Centella asiatica Mediated Synthesised AgNPs Loaded Hybrid Nanofiber Scaffold: In Vitro and In Vivo Studies
dc.typeArticle

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