The effects of cyclohexyladenosine on cold water swim stress (CWSS) induced analgesia
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Tarih
2000
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Cold water swimming induces analgesia on rats and mice. This analgesia is abolished by non-selective ? receptor antagonist naloxone and appears by delta-2 specific ? receptor agonist. The relation between CWSS and adenosine agonists are not investigated. The purpose of this study is to investigate the effect of adenosine A1 receptor agonist cyclohexyladenosine (CHA) on CWSS induced analgesia. 75 Swiss Webster male mice, weighing 25-35 g was included to the investigation. Animals were divided into five equal group in a random order. Research protocol was determined as: 1st injection - 5 min - 3 min CWSS - 2 min - 2nd injection; 10, 20, 30 min after hot plate test (HPT). Vehicle saline (S) solution was administered to the 1st group of mice on both injections. 8 phenylteophylline (8 PT) 50 mg/kg and S was given to the 2nd group of animal. S and CHA 2 mg/kg was injected to the 3rd group of mice. 8 PT 50 mg/kg and CHA 2 mg/kg was administered to the 4th group of animals respectively. On the 5th group, 8 PT 50 mg/kg was given for first injection and CHA 1 ?g/kg (5 ?L) was injected via intracerebroventricular route. All injections were performed with 30 G tipped insulin injector at same volume (0.1 mL) by intraperitoneal or subcutaneous route. Water temperature was determined as 10°C. Mice were placed on 56°C test surface which separated by visible glass chamber. Characteristic fore limb licking, hind limb shaking or withdrawal were taken as positive signs. The period for reaction was determined as latency. CWSS was augmented the systemic effects of CHA. Analgesia was not abolished by non-selective adenosine antagonist 8 PT. CHA alone was not further effect on CWSS induced analgesia. There were no obvious difference between CHA administered at either central and peripheral route on 8 PT pre-treated animals with vehicle group. These results declares that there is no effect of adenosine agonists on CWSS induced analgesia. In conclusion, the analgesia which appeared by CWSS produced ceiling effect. CHA administration did not produce additional analgesia but augmented other systemic effects of adenosine agonists.
Açıklama
Anahtar Kelimeler
8 phenylteophylline, Analgesia, Cold water swim, Cyclohexyladenosine
Kaynak
Turk Anesteziyoloji ve Reanimasyon
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
28
Sayı
4
