Chitosan based metal-chelated copolymer nanoparticles: Laccase immobilization and phenol degradation studies

Basit Öğe Kaydı
dc.contributor.authorAlver, Erol
dc.contributor.authorMetin, Aysegul Ulku
dc.date.accessioned2020-06-25T18:22:38Z
dc.date.available2020-06-25T18:22:38Z
dc.date.issued2017
dc.departmentKırıkkale Üniversitesi
dc.description.abstractImmobilization improves the stability and reusability of enzymes. In this study, laccase, which is a green biocatalyst, was immobilized onto Cu (II)-chelated chitosan nanoparticles via adsorption and successfully applied to remove phenol from aqueous solution. Cu (II)-chelated chitosan-graft-poly (glycidyl methacrylate) nanoparticles were prepared using poly (ethylene imine), PEI, which is employed as both a spacer arm and metal chelator and used to immobilize laccase by coordination. Properties of chitosan based nanoparticles were characterized using FTIR, TGA, SEM and zeta-sizer analysis. The maximum laccase loading capacity of Cu (II)-chelated chitosan based nanoparticles (CHT-PGMA-PEI-Cu (II) NPs) was calculated as 65.75 +/- 2.51 mg/g. This immobilized system exhibited broad pH and temperature profiles, and better stability and reusability than free enzyme; after eight cycles of continuous use, the activity of the immobilized enzyme remained above 50 +/- 0.62%. The K-m and V-max values of free and immobilized laccase were 0.055 mM, 0.070 mM, and 0.19 U/mg, 0.14 U/mg, respectively. The catalytic efficiencies (V-max/K-M) of the free and immobilized laccase were 3.45 and 2.0, respectively. Feasibility of the laccase immobilized system in the removal of phenol was investigated in a batch system. The results showed that the CHT-PGMA-PEI-Cu (II) NPs have great potential for industrial applications. More than 96% of phenol was removed with laccase immobilized metal chelated NPs in the presence of mediator, ABTS, indicating that the metal-chelated chitosan based NPs is a promising support for both laccase immobilization and further applications in the removal of phenolic compounds. (C) 2017 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipKirikkale UniversityKirikkale University [2012/26]en_US
dc.description.sponsorshipThe authors are grateful for research funding by the Research Fund of Kirikkale University (Project code 2012/26).en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.ibiod.2017.07.012
dc.identifier.endpage242en_US
dc.identifier.issn0964-8305
dc.identifier.issn1879-0208
dc.identifier.scopus2-s2.0-85027095920
dc.identifier.scopusqualityQ1
dc.identifier.startpage235en_US
dc.identifier.urihttps://doi.org/10.1016/j.ibiod.2017.07.012
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6845
dc.identifier.volume125en_US
dc.identifier.wosWOS:000414878300026
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofInternational Biodeterioration & Biodegradation
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChitosanen_US
dc.subjectNanoparticlesen_US
dc.subjectLaccaseen_US
dc.subjectCu (II)en_US
dc.subjectPhenolen_US
dc.titleChitosan based metal-chelated copolymer nanoparticles: Laccase immobilization and phenol degradation studiesen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Chitosan based metal-chelated copolymer nanoparticles Laccase immobilization and phenol degradation studies.pdf
Boyut:
1.5 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu