Polyethylenimine-grafted and HSA-immobilized poly(GMA-MMA) affinity adsorbents for bilirubin removal

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dc.contributor.authorArıca, M.Yakup
dc.contributor.authorYalçın, Emine
dc.contributor.authorBayramoğlu, Gülay
dc.date.accessioned2020-06-25T15:13:35Z
dc.date.available2020-06-25T15:13:35Z
dc.date.issued2005
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe epoxy-group-containing microspheres from cross-linked glycidyl methacrylate and methyl methacrylate, poly(GAM-MMA), were prepared by suspension polymerisation. The epoxy groups of the poly(GMA-MMA) microspheres were used for grafting with an anionic polymer polyethylenimine (PEI) to prepare non-specific affinity adsorbents (poly(GMA-MMA)-PEI) for bilirubin removal. The specificity of the poly(GMA-MMA)-PEI adsorbent to bilirubin was further increased by immobilization of human serum albumin (HSA) via adsorption onto PEI-grafted poly(GMA-MMA) adsorbent. Various amounts of HSA were immobilized on the poly(GMA-MMA)-PEI adsorbent by changing the medium pH and initial HSA concentration. The maximum HSA content was obtained at 68.3mg g-1 microspheres. The effects of pH, ionic strength, temperature and initial bilirubin concentration on the adsorption capacity of both adsorbents were investigated in a batch system. Separation of bilirubin from human serum was also investigated in a continuous-flow system. The bilirubin adsorption on the poly(GMA-MMA)-PEI and poly(GMA-MMA)-PEI-HSA was not well described by the Langmuir model, but obeyed the Freundlich isotherm model. The poly(GMA-MMA)-PEI affinity microspheres are stable when subjected to sanitization with sodium hydroxide after repeated adsorption-desorption cycles. © 2004 Society of Chemical Industry.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/pi.1662
dc.identifier.endpage160en_US
dc.identifier.issn09598103
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-11144320410
dc.identifier.scopusqualityQ1
dc.identifier.startpage153en_US
dc.identifier.urihttps://doi.org/10.1002/pi.1662
dc.identifier.urihttps://hdl.handle.net/20.500.12587/1856
dc.identifier.volume54en_US
dc.indekslendigikaynakScopus
dc.language.isoen
dc.relation.ispartofPolymer International
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBilirubinen_US
dc.subjectGraftingen_US
dc.subjectHSAen_US
dc.subjectHydrogelsen_US
dc.subjectImmobilizationen_US
dc.subjectMicrospheresen_US
dc.subjectPEIen_US
dc.titlePolyethylenimine-grafted and HSA-immobilized poly(GMA-MMA) affinity adsorbents for bilirubin removalen_US
dc.typeArticle

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