Novel layer-by-layer self-assembled peptide nanocarriers for siRNA delivery

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dc.contributor.authorBozdogan, Betul
dc.contributor.authorAkbal, Oznur
dc.contributor.authorCelik, Ekin
dc.contributor.authorTurk, Mustafa
dc.contributor.authorDenkbas, Emir Baki
dc.date.accessioned2020-06-25T18:23:29Z
dc.date.available2020-06-25T18:23:29Z
dc.date.issued2017
dc.departmentKırıkkale Üniversitesi
dc.descriptionAKBAL, OZNUR/0000-0003-1972-5987; Bozdogan, Betul/0000-0003-1546-3895; CELIK, EKIN/0000-0003-1966-3907
dc.description.abstractAll complex and functional structures of nature consist of simple building blocks that are thermodynamically balanced and self-assembled at the molecular level. Production of functional bio-nanomaterials with molecular self-assembly mechanisms, based on a bottom-up approach, has become increasingly important in recent years. In this study, a biodegradable and biocompatible siRNA nanocarrier system, consisting of diphenylalaninamide (FFA) based nanoparticles, was developed for silencing of HER2, a gene known to be overexpressed in breast cancer. FFA contains an amide functional group that has a dipolar nature with zero net charge. Here we report an original approach to functionalizing peptide nanoparticles based on layer-by-layer polyelectrolyte deposition (LbL PD) technique. The resulting well-defined FFA nanoparticles (FFANPs) were coated with polycationic poly-Llysine (PLL) by cation-dipole interaction, giving rise to a net positive surface charge. The PLL coating improved the physical stability of FFANPs at physiological pH and temperature. The cationized FFANP was then interacted with the polyanionic siRNA, forming an FFANP-PLL/siRNA complex. Nanoparticles were then interacted with PLL one more time, to create a third layer that can prevent degradation of the siRNA by nucleases and achieve effective delivery of the siRNA into the cytoplasm. These original FFANP-PLL/siRNA/PLL were optimized to achieve efficient in vitro gene silencing. Overall, this study shows that FFANP-PLL/siRNA/PLL are promising gene carriers for gene silencing therapies.en_US
dc.description.sponsorshipRotalab Scientific Instruments Ltd. Co.en_US
dc.description.sponsorshipThe authors gratefully acknowledged the supports from Rotalab Scientific Instruments Ltd. Co.en_US
dc.identifier.citationBozdogan, Betul & Akbal, Oznur & Çelik, Ekin & Türk, Mustafa & Denkbas, Emir. (2017). Novel layer-by-layer self-assembled peptide nanocarriers for siRNA delivery. RSC Adv.. 7. 47592-47601.en_US
dc.identifier.doi10.1039/c7ra08460a
dc.identifier.endpage47601en_US
dc.identifier.issn2046-2069
dc.identifier.issue75en_US
dc.identifier.scopus2-s2.0-85031325981
dc.identifier.scopusqualityQ1
dc.identifier.startpage47592en_US
dc.identifier.urihttps://doi.org/10.1039/c7ra08460a
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7116
dc.identifier.volume7en_US
dc.identifier.wosWOS:000412977000043
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofRsc Advances
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleNovel layer-by-layer self-assembled peptide nanocarriers for siRNA deliveryen_US
dc.typeArticle

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