Poly(2-hydroxyethylmethacrylate)/chitosan dye and different metal-ion-immobilized interpenetrating network membranes: Preparation and application in metal affinity chromatography

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dc.contributor.authorBayramoglu, G
dc.date.accessioned2020-06-25T17:35:29Z
dc.date.available2020-06-25T17:35:29Z
dc.date.issued2003
dc.departmentKırıkkale Üniversitesi
dc.description.abstractComposite membranes were synthesized with 2-hydroxyethylmethacrylate and chitosan (pHEMA/chitosan) via an ultraviolet-initiated photopolymerization technique in the presence of an initiator (alpha,alpha'-azobisisobutyronitrile). The interpenetrating network (IPN) membranes were improved by the immobilization of dye molecules via hydroxyl and amino groups on the membrane surfaces from the IPNs. A triazidine dye (Procion Green H-4G) was covalently immobilized as a ligand onto the IPN membranes. The protein showed various affinities to different chelated metal ions on the membrane surfaces that best matched its own distribution of functional sites, resulting in a distribution of binding energies. In support of this interpretation, two different metal ions, Zn(II) and Fe(III), were chelated with the immobilized dye molecules. The adsorption and binding characteristics of the different metal-ion-chelated dye-immobilized IPN membranes for the lysozyme were investigated with aqueous solutions in magnetically stirred cells. The experimental data were analyzed with two adsorption kinetic models, pseudo-first-order and pseudo-second-order, to determine the best fit equation for the adsorption of lysozyme onto IPN membranes. The second-order equation for the lysozyme-dye-metal-chelated IPN membrane systems was the most appropriate equation for predicting the adsorption capacity for all the tested adsorbents. The reversible lysozyme adsorption on the dye-immobilized and metal-ion-chelated membranes obeyed the Temkin isotherm. The lysozyme adsorption capacity of the pHEMA/chitosan dye, pHEMA/chitosan dye-Zn(II), and pHEMA/chitosan dye-Fe(III) membranes were 2.54, 2.85, and 3.64 mg cm(-2), respectively. The nonspecific adsorption of the lysozyme on the plain pHEMA/chitosan membrane was about 0.18 mg cm(-2). (C) 2003 Wiley Periodicals, Inc.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/app.11844
dc.identifier.endpage1853en_US
dc.identifier.issn0021-8995
dc.identifier.issue7en_US
dc.identifier.scopus2-s2.0-0037449021
dc.identifier.scopusqualityQ2
dc.identifier.startpage1843en_US
dc.identifier.urihttps://doi.org/10.1002/app.11844
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3133
dc.identifier.volume88en_US
dc.identifier.wosWOS:000181520600032
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherJohn Wiley & Sons Incen_US
dc.relation.ispartofJournal Of Applied Polymer Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectinterpenetrating networks (IPN)en_US
dc.subjectmembranesen_US
dc.subjectadsorptionen_US
dc.titlePoly(2-hydroxyethylmethacrylate)/chitosan dye and different metal-ion-immobilized interpenetrating network membranes: Preparation and application in metal affinity chromatographyen_US
dc.typeArticle

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